The crystal structure of di-hydroxyisobutyrate)diaquo neodymium nitrate Nd(C_4H_7O_3)_2NO_32H_2O was determined by X-ray diffraction analysis. It belongs to monoclinic,space group C2/C,with cell dimensionsa=8.996(1), ...The crystal structure of di-hydroxyisobutyrate)diaquo neodymium nitrate Nd(C_4H_7O_3)_2NO_32H_2O was determined by X-ray diffraction analysis. It belongs to monoclinic,space group C2/C,with cell dimensionsa=8.996(1), b=8.601(1),c=21.111(3),β=92.38°,V=1632.O,Z=4,D_c=1.83g/cm^3.Crystal structure has been refined by full-matrix least-square techniques giving a final R value of 0.036.The neodymium atom is coordinated to eight oxygen atoms.The nitrate group in complex is not bonded to neodymium,each two neodymium atoms are linked by bridging carboxyl group forming a chain polymeric molecule.展开更多
Objective To observe the biological function of human 3-hydroxyisobutyrate dehydrogenase(HIBADH).Methods Human 3-hydroxyisobutyrate dehydrogenase(HIBADH, 3-hydroxy-2-methyl propanoate: NAD+ oxidoreductase) recombinant...Objective To observe the biological function of human 3-hydroxyisobutyrate dehydrogenase(HIBADH).Methods Human 3-hydroxyisobutyrate dehydrogenase(HIBADH, 3-hydroxy-2-methyl propanoate: NAD+ oxidoreductase) recombinant protein was expressed in E. coli BL21, and purified by Ni+ column. The special antisera was obtained from rabbits immunized by this purified antigen. On the distribution of HIBADH, it was found that HIBADH over-expressed in the injured liver cells when serious hepatitis occurred. The phenomenon was confirmed in the animal models of SD rats with acute liver cell injury induced by CCl4, but this phenomenon did not exist in the models induced by endotoxin combined with galactosamine. Further more, HIBADH's overexpression in liver cells will induce cell necrosis through the pathway of oxidative stress.Results When the liver cells injured by drug or other chemical materials, HIBADH will be compensationally over-expressed for the deficiency of energy, so liver cells can make enough ATP through brand-chain amino acid catabolism. However, the overexpression of HIBADH will be harmful for liver cells through the product of much more active oxygens which will induce the cell necrosis. Conclusions HIBADH over-expression is a signal of the liver cell metabolism injury, and it can aggravate the liver cell injury through oxidative stress.展开更多
Chiral C2-symmetric N-heterocyclic carbene （NHC） palladium diaquo complex 5b prepared from （S）-BINAM was found to be a fairly effective catalyst for the enantioselective asymmetric fluorination of oxindoles to giv...Chiral C2-symmetric N-heterocyclic carbene （NHC） palladium diaquo complex 5b prepared from （S）-BINAM was found to be a fairly effective catalyst for the enantioselective asymmetric fluorination of oxindoles to give the corresponding products in moderate enantioselectivities along with good to excellent yields.展开更多
文摘The crystal structure of di-hydroxyisobutyrate)diaquo neodymium nitrate Nd(C_4H_7O_3)_2NO_32H_2O was determined by X-ray diffraction analysis. It belongs to monoclinic,space group C2/C,with cell dimensionsa=8.996(1), b=8.601(1),c=21.111(3),β=92.38°,V=1632.O,Z=4,D_c=1.83g/cm^3.Crystal structure has been refined by full-matrix least-square techniques giving a final R value of 0.036.The neodymium atom is coordinated to eight oxygen atoms.The nitrate group in complex is not bonded to neodymium,each two neodymium atoms are linked by bridging carboxyl group forming a chain polymeric molecule.
文摘Objective To observe the biological function of human 3-hydroxyisobutyrate dehydrogenase(HIBADH).Methods Human 3-hydroxyisobutyrate dehydrogenase(HIBADH, 3-hydroxy-2-methyl propanoate: NAD+ oxidoreductase) recombinant protein was expressed in E. coli BL21, and purified by Ni+ column. The special antisera was obtained from rabbits immunized by this purified antigen. On the distribution of HIBADH, it was found that HIBADH over-expressed in the injured liver cells when serious hepatitis occurred. The phenomenon was confirmed in the animal models of SD rats with acute liver cell injury induced by CCl4, but this phenomenon did not exist in the models induced by endotoxin combined with galactosamine. Further more, HIBADH's overexpression in liver cells will induce cell necrosis through the pathway of oxidative stress.Results When the liver cells injured by drug or other chemical materials, HIBADH will be compensationally over-expressed for the deficiency of energy, so liver cells can make enough ATP through brand-chain amino acid catabolism. However, the overexpression of HIBADH will be harmful for liver cells through the product of much more active oxygens which will induce the cell necrosis. Conclusions HIBADH over-expression is a signal of the liver cell metabolism injury, and it can aggravate the liver cell injury through oxidative stress.
文摘Chiral C2-symmetric N-heterocyclic carbene （NHC） palladium diaquo complex 5b prepared from （S）-BINAM was found to be a fairly effective catalyst for the enantioselective asymmetric fluorination of oxindoles to give the corresponding products in moderate enantioselectivities along with good to excellent yields.
