BACKGROUND Previous studies have found that hyperuricaemia(HUA)is closely related to intestinal flora imbalance.AIM The current study investigated the effects and safety of washed microbiota transplantation(WMT)on ser...BACKGROUND Previous studies have found that hyperuricaemia(HUA)is closely related to intestinal flora imbalance.AIM The current study investigated the effects and safety of washed microbiota transplantation(WMT)on serum uric acid(SUA)levels in different populations.METHODS A total of 144 patients who received WMT from July 2016 to April 2020 in the First Affiliated Hospital of Guangdong Pharmaceutical University and had SUA data before treatment were selected.Changes in SUA levels before and after treatment were retrospectively reviewed based on short-term and mid-term effects of WMT regimens.SUA levels measured in the last test within 3 mo after the first WMT represented the short-term effect,and SUA levels measured in the last test within 3-6 mo after the first WMT represented the mid-term effect.The patients were divided into an HUA group(SUA>416μM)and a normal uric acid(NUA)group(SUA≥202μM to≤416μM)based on pretreatment SUA levels.RESULTS Average short-term SUA levels in the HUA group decreased after WMT(481.00±99.85 vs 546.81±109.64μM,n=32,P<0.05)in 25/32 patients and returned to normal in 10/32 patients.The shortterm level of SUA reduction after treatment moderately correlated with SUA levels before treatment(r=0.549,R²=0.300,P<0.05).Average SUA levels decreased after the first and second courses of WMT(469.74±97.68 vs 540.00±107.16μM,n=35,and 465.57±88.88 vs 513.19±78.14μM,n=21,P<0.05).Short-term and mid-term SUA levels after WMT and SUA levels after the first,second and third courses of WMT were similar to the levels before WMT in the NUA group(P>0.05).Only 1/144 patients developed mild diarrhea after WMT.CONCLUSION WMT reduces short-term SUA levels in patients with HUA with mild side effects but has no obvious effect on SUA levels in patients with NUA.展开更多
Objective:To study the effect of retention enema of Chinese herbal medicine combined with allopurinol in treating hyperuricaemia(HUE).Methods:Seventy-eight patients with HUE were assigned to two groups,the 40 patients...Objective:To study the effect of retention enema of Chinese herbal medicine combined with allopurinol in treating hyperuricaemia(HUE).Methods:Seventy-eight patients with HUE were assigned to two groups,the 40 patients in the treated group were treated with retention enema of Chinese herbal medicine combined with oral intake of allopurinol,and the 38 patients in the control group were treated with allopurinol alone. The therapeutic course for all was 6 weeks.The clinical efficacy,changes of symptoms,blood le...展开更多
目的:系统评价健康筛查发生高尿酸血症风险预测模型,为其防治和进一步研究提供参考依据。方法:计算机检索The Cochrane Library、PubMed、Web of Science、维普、万方和知网数据库中有关健康筛查发生高尿酸血症风险预测模型的文献,检索...目的:系统评价健康筛查发生高尿酸血症风险预测模型,为其防治和进一步研究提供参考依据。方法:计算机检索The Cochrane Library、PubMed、Web of Science、维普、万方和知网数据库中有关健康筛查发生高尿酸血症风险预测模型的文献,检索时限为建库至2021年12月10日。由2名研究者独立进行文献筛选和数据提取,并参照预测模型研究的偏倚风险评估工具PROBAST对纳入研究进行偏倚风险和适用性评价,对纳入研究的研究设计、样本量、预测因子、建模方法、验模方法、模型性能等结果进行比较和分析。结果:本研究共纳入14项研究,12项为开发研究。14项研究的ROC曲线下面积在0.512~0.827,其中有3项模型区分度极佳(>0.80)。模型纳入前5的预测因子包括:身体质量指数、年龄、甘油三酯、性别、总胆固醇。12项开发研究的适用性较好,但14项研究偏倚风险高,主要原因是变量转化尚未进行规范处理、忽略缺失值、单因素分析筛选预测因子、尚未汇报数据复杂性问题、缺乏模型性能评估。结论:健康筛查发生高尿酸血症风险预测模型整体偏倚风险较高,研究尚处于发展阶段,需要参照PROBAST评估工具和TRIPOD报告声明开发高质量的模型,以便今后能尽早识别高尿酸血症风险并及时采取预防措施。展开更多
痛风是由高尿酸血症引发的一种常见炎性关节炎,受遗传因素和环境因素共同作用。早期研究表明,PRPS1和HPRT1等单基因稀有突变会引起嘌呤合成代谢紊乱,从而引发高尿酸血症和痛风。近年来,全基因组关联分析(Genome-wide association studie...痛风是由高尿酸血症引发的一种常见炎性关节炎,受遗传因素和环境因素共同作用。早期研究表明,PRPS1和HPRT1等单基因稀有突变会引起嘌呤合成代谢紊乱,从而引发高尿酸血症和痛风。近年来,全基因组关联分析(Genome-wide association studies,GWAS)已检出多个导致高尿酸血症和痛风的易感位点及相关候选基因。其中SLC2A9、SLC22A11和SLC22A12基因功能缺失性突变可引起遗传性低尿酸血症,而过表达则会加强尿酸的重吸收。ABCG2、SLC17A1和SLC17A3基因功能缺陷型变异会降低肾脏和肠道对尿酸的排泄量。因此,诱发尿酸排泄障碍(高重吸收和低排泄)的基因变异是影响高尿酸血症和痛风的主要遗传因素。另外,抑制-激活生长因子系统、转录因子、细胞骨架以及基因和环境的互作等因素也一定程度影响血液尿酸水平。在中国汉族人群中,两个新发现的易感基因RFX3和KCNQ1可能造成免疫应答受损和胰岛B细胞功能缺陷,从而直接或间接引起高尿酸酸血症和痛风。本文系统综述了高尿酸血症和痛风的遗传学研究,以促进人们对高尿酸血症和痛风发病机理的理解。展开更多
基金Supported by the Innovation and Entrepreneurship Training Program for College Students of Guangdong Province,No. S201910573028
文摘BACKGROUND Previous studies have found that hyperuricaemia(HUA)is closely related to intestinal flora imbalance.AIM The current study investigated the effects and safety of washed microbiota transplantation(WMT)on serum uric acid(SUA)levels in different populations.METHODS A total of 144 patients who received WMT from July 2016 to April 2020 in the First Affiliated Hospital of Guangdong Pharmaceutical University and had SUA data before treatment were selected.Changes in SUA levels before and after treatment were retrospectively reviewed based on short-term and mid-term effects of WMT regimens.SUA levels measured in the last test within 3 mo after the first WMT represented the short-term effect,and SUA levels measured in the last test within 3-6 mo after the first WMT represented the mid-term effect.The patients were divided into an HUA group(SUA>416μM)and a normal uric acid(NUA)group(SUA≥202μM to≤416μM)based on pretreatment SUA levels.RESULTS Average short-term SUA levels in the HUA group decreased after WMT(481.00±99.85 vs 546.81±109.64μM,n=32,P<0.05)in 25/32 patients and returned to normal in 10/32 patients.The shortterm level of SUA reduction after treatment moderately correlated with SUA levels before treatment(r=0.549,R²=0.300,P<0.05).Average SUA levels decreased after the first and second courses of WMT(469.74±97.68 vs 540.00±107.16μM,n=35,and 465.57±88.88 vs 513.19±78.14μM,n=21,P<0.05).Short-term and mid-term SUA levels after WMT and SUA levels after the first,second and third courses of WMT were similar to the levels before WMT in the NUA group(P>0.05).Only 1/144 patients developed mild diarrhea after WMT.CONCLUSION WMT reduces short-term SUA levels in patients with HUA with mild side effects but has no obvious effect on SUA levels in patients with NUA.
文摘Objective:To study the effect of retention enema of Chinese herbal medicine combined with allopurinol in treating hyperuricaemia(HUE).Methods:Seventy-eight patients with HUE were assigned to two groups,the 40 patients in the treated group were treated with retention enema of Chinese herbal medicine combined with oral intake of allopurinol,and the 38 patients in the control group were treated with allopurinol alone. The therapeutic course for all was 6 weeks.The clinical efficacy,changes of symptoms,blood le...
文摘目的:系统评价健康筛查发生高尿酸血症风险预测模型,为其防治和进一步研究提供参考依据。方法:计算机检索The Cochrane Library、PubMed、Web of Science、维普、万方和知网数据库中有关健康筛查发生高尿酸血症风险预测模型的文献,检索时限为建库至2021年12月10日。由2名研究者独立进行文献筛选和数据提取,并参照预测模型研究的偏倚风险评估工具PROBAST对纳入研究进行偏倚风险和适用性评价,对纳入研究的研究设计、样本量、预测因子、建模方法、验模方法、模型性能等结果进行比较和分析。结果:本研究共纳入14项研究,12项为开发研究。14项研究的ROC曲线下面积在0.512~0.827,其中有3项模型区分度极佳(>0.80)。模型纳入前5的预测因子包括:身体质量指数、年龄、甘油三酯、性别、总胆固醇。12项开发研究的适用性较好,但14项研究偏倚风险高,主要原因是变量转化尚未进行规范处理、忽略缺失值、单因素分析筛选预测因子、尚未汇报数据复杂性问题、缺乏模型性能评估。结论:健康筛查发生高尿酸血症风险预测模型整体偏倚风险较高,研究尚处于发展阶段,需要参照PROBAST评估工具和TRIPOD报告声明开发高质量的模型,以便今后能尽早识别高尿酸血症风险并及时采取预防措施。
文摘痛风是由高尿酸血症引发的一种常见炎性关节炎,受遗传因素和环境因素共同作用。早期研究表明,PRPS1和HPRT1等单基因稀有突变会引起嘌呤合成代谢紊乱,从而引发高尿酸血症和痛风。近年来,全基因组关联分析(Genome-wide association studies,GWAS)已检出多个导致高尿酸血症和痛风的易感位点及相关候选基因。其中SLC2A9、SLC22A11和SLC22A12基因功能缺失性突变可引起遗传性低尿酸血症,而过表达则会加强尿酸的重吸收。ABCG2、SLC17A1和SLC17A3基因功能缺陷型变异会降低肾脏和肠道对尿酸的排泄量。因此,诱发尿酸排泄障碍(高重吸收和低排泄)的基因变异是影响高尿酸血症和痛风的主要遗传因素。另外,抑制-激活生长因子系统、转录因子、细胞骨架以及基因和环境的互作等因素也一定程度影响血液尿酸水平。在中国汉族人群中,两个新发现的易感基因RFX3和KCNQ1可能造成免疫应答受损和胰岛B细胞功能缺陷,从而直接或间接引起高尿酸酸血症和痛风。本文系统综述了高尿酸血症和痛风的遗传学研究,以促进人们对高尿酸血症和痛风发病机理的理解。
