Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocyte...Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.展开更多
A C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst was synthesized using a one-step sol-gel method with silica gel(SG)as the carrier and C_(9)H_(10)O_(2):0.5ZnCl_(2)deep eutectic solvent(DES)as active component.The structure ...A C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst was synthesized using a one-step sol-gel method with silica gel(SG)as the carrier and C_(9)H_(10)O_(2):0.5ZnCl_(2)deep eutectic solvent(DES)as active component.The structure of the supported catalyst was characterized by FT-IR,XRD,SEM,and N2 adsorption-desorption,and the DES was found to have successfully permeated the SG through its pores.The removal of dibenzothiophene(DBT)in model diesel was studied using C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG as a catalyst and H_(2)O_(2)as an oxidant.The influence of loading dose of DES,reaction temperature,catalyst dosage,O/S molar ratio,and sulfide type on the desulfurization rate was investigated.The removal rates of DBT,4,6-dimethyldibenzothiophene(4,6-DMDBT),and benzothiophene(BT)under optimal reaction conditions were 99.4%,96%,and 78.2%,respectively.C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst could be recycled five times with a little decrease of oxidative desulfurization activity,and the adsorption-oxidation desulfurization mechanism was examined.展开更多
The conversion of CO_(2)into value-added chemicals coupled with the storage of intermittent renewable electricity is attractive.CuO nanosheets with an average size and thickness of~30 and~20 nm have been developed,whi...The conversion of CO_(2)into value-added chemicals coupled with the storage of intermittent renewable electricity is attractive.CuO nanosheets with an average size and thickness of~30 and~20 nm have been developed,which are in situ reduced into Cu nanosheets during electrochemical CO_(2)reduction reaction(ECO_(2)RR).The derived Cu nanosheets demonstrate much higher selectivity for C2H4production than commercial CuO derived Cu powder,with an optimum Faradaic efficiency of 56.2%and a partial current density of C_(2)H_(4)as large as 171.0 mA cm^(-2)in a gas diffusion flow cell.The operando attenuated total reflectance-Fourier transform infrared spectra measurements and density functional theory simulations illustrate that the high activity and selectivity of Cu nanosheets originate from the edge sites on Cu nanosheets with a coordinate number around 5(4–6),which facilitates the formation of^(*)CHO rather than^(*)COH intermediate,meanwhile boosting the C-C coupling reaction of^(*)CO and^(*)CHO intermediates,which are the critical steps for C_(2)H_(4)formation.展开更多
基金National Natural Science Foundation of China(No.82160597)Guangxi Natural Science Foundation Project(No.2020GXNSFAA159148)。
文摘Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.
基金the financial support of the Natural Science Foundation of Liaoning Province(2019-ZD-0064)the Doctoral Fund of Liaoning Province(201501105).
文摘A C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst was synthesized using a one-step sol-gel method with silica gel(SG)as the carrier and C_(9)H_(10)O_(2):0.5ZnCl_(2)deep eutectic solvent(DES)as active component.The structure of the supported catalyst was characterized by FT-IR,XRD,SEM,and N2 adsorption-desorption,and the DES was found to have successfully permeated the SG through its pores.The removal of dibenzothiophene(DBT)in model diesel was studied using C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG as a catalyst and H_(2)O_(2)as an oxidant.The influence of loading dose of DES,reaction temperature,catalyst dosage,O/S molar ratio,and sulfide type on the desulfurization rate was investigated.The removal rates of DBT,4,6-dimethyldibenzothiophene(4,6-DMDBT),and benzothiophene(BT)under optimal reaction conditions were 99.4%,96%,and 78.2%,respectively.C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst could be recycled five times with a little decrease of oxidative desulfurization activity,and the adsorption-oxidation desulfurization mechanism was examined.
基金funded by the National Key Research and Development Program of China(2017YFA0700103,2018YFA0704502)the National Natural Science Foundation of China(21703248)staffs in BL11B beamline in Shanghai Synchrotron Radiation Facility(SSRF)for their technical assistance(2020-SSRF-PT-012223 and 2021-SSRF-PT-015319)。
文摘The conversion of CO_(2)into value-added chemicals coupled with the storage of intermittent renewable electricity is attractive.CuO nanosheets with an average size and thickness of~30 and~20 nm have been developed,which are in situ reduced into Cu nanosheets during electrochemical CO_(2)reduction reaction(ECO_(2)RR).The derived Cu nanosheets demonstrate much higher selectivity for C2H4production than commercial CuO derived Cu powder,with an optimum Faradaic efficiency of 56.2%and a partial current density of C_(2)H_(4)as large as 171.0 mA cm^(-2)in a gas diffusion flow cell.The operando attenuated total reflectance-Fourier transform infrared spectra measurements and density functional theory simulations illustrate that the high activity and selectivity of Cu nanosheets originate from the edge sites on Cu nanosheets with a coordinate number around 5(4–6),which facilitates the formation of^(*)CHO rather than^(*)COH intermediate,meanwhile boosting the C-C coupling reaction of^(*)CO and^(*)CHO intermediates,which are the critical steps for C_(2)H_(4)formation.
