Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocyte...Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.展开更多
A C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst was synthesized using a one-step sol-gel method with silica gel(SG)as the carrier and C_(9)H_(10)O_(2):0.5ZnCl_(2)deep eutectic solvent(DES)as active component.The structure ...A C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst was synthesized using a one-step sol-gel method with silica gel(SG)as the carrier and C_(9)H_(10)O_(2):0.5ZnCl_(2)deep eutectic solvent(DES)as active component.The structure of the supported catalyst was characterized by FT-IR,XRD,SEM,and N2 adsorption-desorption,and the DES was found to have successfully permeated the SG through its pores.The removal of dibenzothiophene(DBT)in model diesel was studied using C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG as a catalyst and H_(2)O_(2)as an oxidant.The influence of loading dose of DES,reaction temperature,catalyst dosage,O/S molar ratio,and sulfide type on the desulfurization rate was investigated.The removal rates of DBT,4,6-dimethyldibenzothiophene(4,6-DMDBT),and benzothiophene(BT)under optimal reaction conditions were 99.4%,96%,and 78.2%,respectively.C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst could be recycled five times with a little decrease of oxidative desulfurization activity,and the adsorption-oxidation desulfurization mechanism was examined.展开更多
Two novel clusters [Mn~Ⅲ_3（μ_3-O）（phendox）3]X·13H_2O（X = Cl（1）, Br（2]） have been obtained from the solvothermal reactions of 1,10-phenanthroline-2,9-dicarbaldehyde dioxime（H_2phendox） with MnCl_2...Two novel clusters [Mn~Ⅲ_3（μ_3-O）（phendox）3]X·13H_2O（X = Cl（1）, Br（2]） have been obtained from the solvothermal reactions of 1,10-phenanthroline-2,9-dicarbaldehyde dioxime（H_2phendox） with MnCl_2·4H_2O or anhydrous MnBr_2, and their structures were characterized by elemental analysis, FT-IR, XRD, TGA, MS and single-crystal X-ray diffraction. It crystallizes in trigonal, space group P3_1/c. X-ray analysis reveals that the neighbouring [Mn_3（μ_3-O）（phendox）_3]＋ cores are linked by C–H···Cl hydrogen bonds and form an infinite supramolecular chain along the c-axis. Neighbouring chains are packed with each other by off-set p-p interactions of the aromatic rings on phenox2-. A 3D supramolecular architecture in a honeycomb topology is formed with 1D hexagonal channel in the dimensions of 13？ × 13？ along the c-axis. The gas adsorption studies show that compound 1·13H_2O is stable upon the removal of guest molecules and the desolvated compound absorbed considerable amount of CO_2.展开更多
目的:利用成簇规律性间隔短回文重复序列(clustered regularly interspaced short palindromic repeat,CRISPR)相关蛋白9(CRISP associated protein 9,Cas9)技术构建微小核糖核酸-551b(miR-551b)基因敲除小鼠模型。方法:选择健康C57BL/6...目的:利用成簇规律性间隔短回文重复序列(clustered regularly interspaced short palindromic repeat,CRISPR)相关蛋白9(CRISP associated protein 9,Cas9)技术构建微小核糖核酸-551b(miR-551b)基因敲除小鼠模型。方法:选择健康C57BL/6J小鼠,针对miR-551b外显子1区域,设计导向RNA(guide RNA,gRNA),构建Cas9载体质粒,将体外转录的Cas9 RNA及gRNA显微注射入小鼠的受精卵并体外培养。将培养合格的胚胎移植到代孕小鼠的输卵管中,待小鼠生育后得到F0代小鼠,使用基因测序确定基因敲除情况,与野生型小鼠繁育后,得到F1代杂合小鼠,F1代小鼠经自交繁育获得F2代小鼠,F3代小鼠由F2代纯合小鼠自交获得,采用电泳鉴定小鼠基因型,RT-PCR检测F3代小鼠组织miR-551b的表达。结果:利用CRISPR/Cas9技术构建模型小鼠得到F0代小鼠,通过测序筛选出缺失目标序列的F0代杂合子小鼠。与WT小鼠繁育后,琼脂糖凝胶电泳及测序筛选出F1代杂合小鼠,同样的方法鉴定并获得F2、F3代基因敲除小鼠,获取F3代纯合小鼠的心脏及下腔静脉样本,RT-PCR结果证实F3代纯合小鼠miR551b表达明显低于WT小鼠(P<0.05),成功敲除miR-551b基因。结论:通过CRISPR-Cas9技术成功构建miR⁃155基因敲除小鼠模型并稳定遗传,为进一步研究提供了有利条件。展开更多
基金National Natural Science Foundation of China(No.82160597)Guangxi Natural Science Foundation Project(No.2020GXNSFAA159148)。
文摘Objective: To explore the protective effect of camellia oil against H2O2-induced oxidative stress injury in rat H9C2 cardiomyocytes. Methods: CCK8 method was used to detect the cell survival rate of H9C2 cardiomyocytes treated with different concentrations of H2O2. Normal cultured cells were used as the blank control group, and the cells were treated with 200 μmol/L H2O2 for 24 h. An oxidative stress injury model was constructed as the model group. The cells were pretreated with 1%, 0.1% and 0.01% camellia oil for 24 h, and then H2O2 was added for 24 h as the experimental group. The β-galactosidase senescence staining assay, mitochondrial membrane potential assay, EdU cell proliferation staining assay and scratch assay were used to observe the changes of cell senescence, mitochondrial membrane potential, proliferation, apoptosis and migration in each group. The superoxide dismutase (SOD) activity, lactate dehydrogenase (LDH) activity, and malondialdehyde (MDA) content of the cells in each group were detected by using the kit. Results: The cell viability of H9C2 cardiomyocytes treated with different concentrations of H2O2 was inhibited and positively correlated with the concentration of H2O2 (P<0.01). Compared with the blank control group, the positive rate of cell senescence, MDA content and LDH activity increased in the H2O2 model group (P<0.01);mitochondrial membrane potential, cellular value-added rate, migration rate and SOD activity decreased (P<0.01). Compared with the H2O2 model group, the positive rate of cellular senescence (P<0.01 or P<0.05), MDA content and LDH activity decreased (P< 0.01 or P<0.05);mitochondrial membrane potential increased, cell proliferation rate and migration rate increased (P<0.01 or P<0.05) in the experimental group. Conclusion: Camellia oil can significantly inhibit oxidative stress injury in H9C2 cells and exert cardiomyocyte protective effects.
基金This work is supported by the National Natural Science Foundation of China(No.22103075,No.21827804,and No.22173089)the National Key R&D Program of China(No.2021YFA0716801andNo.2017YFA0303502).
基金the financial support of the Natural Science Foundation of Liaoning Province(2019-ZD-0064)the Doctoral Fund of Liaoning Province(201501105).
文摘A C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst was synthesized using a one-step sol-gel method with silica gel(SG)as the carrier and C_(9)H_(10)O_(2):0.5ZnCl_(2)deep eutectic solvent(DES)as active component.The structure of the supported catalyst was characterized by FT-IR,XRD,SEM,and N2 adsorption-desorption,and the DES was found to have successfully permeated the SG through its pores.The removal of dibenzothiophene(DBT)in model diesel was studied using C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG as a catalyst and H_(2)O_(2)as an oxidant.The influence of loading dose of DES,reaction temperature,catalyst dosage,O/S molar ratio,and sulfide type on the desulfurization rate was investigated.The removal rates of DBT,4,6-dimethyldibenzothiophene(4,6-DMDBT),and benzothiophene(BT)under optimal reaction conditions were 99.4%,96%,and 78.2%,respectively.C_(9)H_(10)O_(2):0.5ZnCl_(2)/SG catalyst could be recycled five times with a little decrease of oxidative desulfurization activity,and the adsorption-oxidation desulfurization mechanism was examined.
基金supported by Guangdong Ocean University with doctor start-up fund(1112263)the Science and Technology Plan Projects of Zhanjiang City(No.2013B01073)
文摘Two novel clusters [Mn~Ⅲ_3（μ_3-O）（phendox）3]X·13H_2O（X = Cl（1）, Br（2]） have been obtained from the solvothermal reactions of 1,10-phenanthroline-2,9-dicarbaldehyde dioxime（H_2phendox） with MnCl_2·4H_2O or anhydrous MnBr_2, and their structures were characterized by elemental analysis, FT-IR, XRD, TGA, MS and single-crystal X-ray diffraction. It crystallizes in trigonal, space group P3_1/c. X-ray analysis reveals that the neighbouring [Mn_3（μ_3-O）（phendox）_3]＋ cores are linked by C–H···Cl hydrogen bonds and form an infinite supramolecular chain along the c-axis. Neighbouring chains are packed with each other by off-set p-p interactions of the aromatic rings on phenox2-. A 3D supramolecular architecture in a honeycomb topology is formed with 1D hexagonal channel in the dimensions of 13？ × 13？ along the c-axis. The gas adsorption studies show that compound 1·13H_2O is stable upon the removal of guest molecules and the desolvated compound absorbed considerable amount of CO_2.
