Hadamard transform spatial multiplexed imaging technique is combined with fluorescence microscope and an instrument of Hadamard transform microscope fluorescence image analysis is developed. Images acquired by this in...Hadamard transform spatial multiplexed imaging technique is combined with fluorescence microscope and an instrument of Hadamard transform microscope fluorescence image analysis is developed. Images acquired by this instrument can provide a lot of useful information simultaneously, including three-dimensional Hadamard transform microscope cell fluorescence image, the fluorescence intensity and fluorescence distribution of a cell, the background signal intensity and the signal/noise ratio, etc.展开更多
A novel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexin...A novel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is performed with a 511 slit one dimensional Hadamard mask and a highly sensitive linear CCD. The weak native fluorescence imaging for Zephyranthes candida (Lindl.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 0 6 μm per pixel has been achieved with a 40× objective. The imaging speed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily.展开更多
A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was ...A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was employed to trace the interaction of drugand cellular DNA. The results for five anticancer drugs, vincristine, cyclophosphamide,mustine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this method is suitable for screening cell cyclenonspecific anticancer drugs.展开更多
The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The resu...The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The results show that the system can be applied for high-resolution single-cell imaging sensitively in a short time, the cell image provided by the system has a high-resolution up to 511×512 pixel. The results also shows that this system has a more excellent resolving power than conventional optical microscope, under a 100× objective(oil lens) the spatial resolution up to 0.24 μm per pixel in X direction was obtained, which is close to the resolution limit of conventional optical microscope. The system was used for multi-parameter evaluation of breast tumor malignancy and malignant grade for a single tumor cell based on nuclei DNA ploidy measurements, and satisfactory results were obtained.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘Hadamard transform spatial multiplexed imaging technique is combined with fluorescence microscope and an instrument of Hadamard transform microscope fluorescence image analysis is developed. Images acquired by this instrument can provide a lot of useful information simultaneously, including three-dimensional Hadamard transform microscope cell fluorescence image, the fluorescence intensity and fluorescence distribution of a cell, the background signal intensity and the signal/noise ratio, etc.
文摘A novel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is performed with a 511 slit one dimensional Hadamard mask and a highly sensitive linear CCD. The weak native fluorescence imaging for Zephyranthes candida (Lindl.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 0 6 μm per pixel has been achieved with a 40× objective. The imaging speed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily.
文摘A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was employed to trace the interaction of drugand cellular DNA. The results for five anticancer drugs, vincristine, cyclophosphamide,mustine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this method is suitable for screening cell cyclenonspecific anticancer drugs.
文摘The imaging capability(sensitivity, signal-to-noise ratio, imaging speed and spatial resolution) of a domestic-made high-resolution Hadamrd transform(HT) microscopic fluorescence imaging system was discussed. The results show that the system can be applied for high-resolution single-cell imaging sensitively in a short time, the cell image provided by the system has a high-resolution up to 511×512 pixel. The results also shows that this system has a more excellent resolving power than conventional optical microscope, under a 100× objective(oil lens) the spatial resolution up to 0.24 μm per pixel in X direction was obtained, which is close to the resolution limit of conventional optical microscope. The system was used for multi-parameter evaluation of breast tumor malignancy and malignant grade for a single tumor cell based on nuclei DNA ploidy measurements, and satisfactory results were obtained.
