Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri(H. barteri).Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadox...Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri(H. barteri).Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine(1.2 mg/kg), aqueous extract(30, 100, 300 mg/kg) and dichloromethane/methanol(D/M)(30, 100, 300 mg/kg) extracts of H. barteri for 3 days. On the 4th day, the mice were inoculated with Plasmodium berghei. The parasite density was estimated for each mouse 72 h post-parasite inoculation. The curative activity of the plant was also performed by inoculating mice with Plasmodium berghei. Three days later, they were treated with artemether-lumefantrine(4 mg/kg), aqueous and D/M extracts of H. barteri stem bark for 5 days. The in vitro antioxidant property of the aqueous extract was determined by using the reducing power, nitric oxide and total antioxidant capacity assays. Results: The aqueous extract exerted significant(P 【 0.05) curative and prophylactic antimalarial activities. The D/M extract exhibited significant curative(P 【 0.05) but not prophylactic antiplasmodial ef ect. The aqueous extract exhibited in vitro antioxidant property with IC50’s of(0.930 ± 0.021) mg/mL,(0.800 ± 0.001) mg/mL and(0.22 ± 0.05) mg/mL in the total antioxidant capacity, reducing power and nitric oxide assays. Histological assessment of the liver of aqueous and D/M treated animals did not reveal any sign of toxicity.Conclusions: H. barteri is not toxic which exerted significant curative antiplasmodial ef ects but the prophylactic property was however fraction dependent. The mechanism of the antiplasmodial activity of H. barteri may partly be mediated by its antioxidant property.展开更多
Understanding species morphological variation across geographical ranges can serve as a first step for germplasm collection and the conservation of genetic resources.The morphological variability of Haematostaphis bar...Understanding species morphological variation across geographical ranges can serve as a first step for germplasm collection and the conservation of genetic resources.The morphological variability of Haematostaphis barteri Hook.F.(Anacardiaceae)in Benin(West Africa)was studied,using 11 traits related to panicles,fruits,leaves and leaflets.A total of 1,485 panicles,1,485 fruits,990 leaves and 4,950 leaflets were sampled.It was found that the sample provenance and site topography have a significant influence on the morphological traits.The discriminating morphological traits were the length of the panicles,the number of fruits per panicle,the length and width of fruits,the mass of the fresh fruit pulp mass,the length of leaves and petioles,the number of leaflets per leaf,length and width of leaflets.On the basis of these traits,four different morphotypes of H.barteri were identified,with however a small intra and inter group variability.The morphotype from sites established on the tops and the high slopes of hill produced larger and heavier fruits with higher pulp mass.This morphotype could be of interest for future varietal selection programs for the species in Benin.展开更多
文摘Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri(H. barteri).Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine(1.2 mg/kg), aqueous extract(30, 100, 300 mg/kg) and dichloromethane/methanol(D/M)(30, 100, 300 mg/kg) extracts of H. barteri for 3 days. On the 4th day, the mice were inoculated with Plasmodium berghei. The parasite density was estimated for each mouse 72 h post-parasite inoculation. The curative activity of the plant was also performed by inoculating mice with Plasmodium berghei. Three days later, they were treated with artemether-lumefantrine(4 mg/kg), aqueous and D/M extracts of H. barteri stem bark for 5 days. The in vitro antioxidant property of the aqueous extract was determined by using the reducing power, nitric oxide and total antioxidant capacity assays. Results: The aqueous extract exerted significant(P 【 0.05) curative and prophylactic antimalarial activities. The D/M extract exhibited significant curative(P 【 0.05) but not prophylactic antiplasmodial ef ect. The aqueous extract exhibited in vitro antioxidant property with IC50’s of(0.930 ± 0.021) mg/mL,(0.800 ± 0.001) mg/mL and(0.22 ± 0.05) mg/mL in the total antioxidant capacity, reducing power and nitric oxide assays. Histological assessment of the liver of aqueous and D/M treated animals did not reveal any sign of toxicity.Conclusions: H. barteri is not toxic which exerted significant curative antiplasmodial ef ects but the prophylactic property was however fraction dependent. The mechanism of the antiplasmodial activity of H. barteri may partly be mediated by its antioxidant property.
文摘Understanding species morphological variation across geographical ranges can serve as a first step for germplasm collection and the conservation of genetic resources.The morphological variability of Haematostaphis barteri Hook.F.(Anacardiaceae)in Benin(West Africa)was studied,using 11 traits related to panicles,fruits,leaves and leaflets.A total of 1,485 panicles,1,485 fruits,990 leaves and 4,950 leaflets were sampled.It was found that the sample provenance and site topography have a significant influence on the morphological traits.The discriminating morphological traits were the length of the panicles,the number of fruits per panicle,the length and width of fruits,the mass of the fresh fruit pulp mass,the length of leaves and petioles,the number of leaflets per leaf,length and width of leaflets.On the basis of these traits,four different morphotypes of H.barteri were identified,with however a small intra and inter group variability.The morphotype from sites established on the tops and the high slopes of hill produced larger and heavier fruits with higher pulp mass.This morphotype could be of interest for future varietal selection programs for the species in Benin.
