Two components of anticoagulant protein were isolated from the leech Haemendipsa yanyuanensis by heparin agarose affinity chromatography and ultracentrifugation. The determination of anticoagulant activity and cha...Two components of anticoagulant protein were isolated from the leech Haemendipsa yanyuanensis by heparin agarose affinity chromatography and ultracentrifugation. The determination of anticoagulant activity and characterization analysis of the protein using the method of chromogenic substrate indicates that the anticoagulant protein is thrombin specific but not factor Xa specific. The results lay a foundation for the research of the anticoagulant mechanism and application of anticoagulant protein from H. yanyuanensis.展开更多
文摘Two components of anticoagulant protein were isolated from the leech Haemendipsa yanyuanensis by heparin agarose affinity chromatography and ultracentrifugation. The determination of anticoagulant activity and characterization analysis of the protein using the method of chromogenic substrate indicates that the anticoagulant protein is thrombin specific but not factor Xa specific. The results lay a foundation for the research of the anticoagulant mechanism and application of anticoagulant protein from H. yanyuanensis.
