Effect and Mechanism of Hai Tong-pi Decoction Ointment on Antiinflammation and Analgesia by Transcutaneous Administration in Mice

Objectve:To investigate the effect and mechanism of Hai Tongpi decoction ointment on analgesic and anti-inflammatory effects in mice after transdermal administration.Methods:The hot plate method and the acetic acid writhing experiment were used to verify the analgesic effect of Hai tong-pi extract on pain model mice;the xylene-induced auricular swelling model and the carrageenan-induced right foot swelling model were used,combined with the ELISA method to determine the analgesic effect.The levels of TNF-α,IL-1βand IL-6 in the serum of mice were observed.Anti-inflammatory effect of Haitongpi Tang decoction ointment on inflammation model mice.Results:In terms of analgesia,compared with the model group,both the treatment group 1 and the treatment group 2 can significantly prolong the hot plate pain threshold and the latency of the writhing response(P<0.05),and significantly reduce the writhing in the mice.The number of times(P<0.001),and the effect of process 1 is more obvious(P<0.05);In terms of antiinflammatory,compared with the blank group,the swelling degree and swelling rate of the right hind paw of the mice in the model group were significantly increased(P<0.01),indicating that the acute inflammation model was successfully established in the mice.Compared with the model group,both the process group 1 and the process group 2 can significantly reduce the swelling degree and swelling rate of the mice feet(P<0.05),and reduce the auricle swelling degree of the mice,and increase the swelling inhibition rate(P<0.05),and the effective effect of process 1 group was more significant(P<0.05).At the same time,compared with the blank group,the contents of TNF-α,IL-1βand IL-6 in the model group were significantly increased(P<0.01).Compared with the process group 2,the process group 1 had a more significant decrease in the content of IL-1β(P<0.05),and IL-6 content of the two had no statistical difference(P>0.05).Conclusion:The Hai tong-pi decoction ointment prepared by two different processes have good analgesic and antiinflammatory effects on mice after transdermal administration.inflammatory cytokine expression.The anti-inflammatory and analgesic effect of process group 1 was better than that of process group 2,which may be related to the separate extraction and storage of volatile oil of traditional Chinese medicine,which ensured the long-term pharmacological activity of volatile oil.

海桐皮汤提取膏经皮给药对小鼠抗炎镇痛作用及机制研究

目的:探讨海桐皮汤提取膏经皮给药后对小鼠镇痛、抗炎作用的影响及其机制。方法:应用热板法、醋酸扭体实验以验证海桐皮汤提取膏对疼痛模型小鼠的镇痛作用;采用二甲苯致耳廓肿胀模型、角叉菜胶致右足肿胀模型,结合ELISA法测定小鼠血清中肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白介素1β(interleukin-1β,IL-1β)、白介素6(interleukin-6,IL-6)的水平,观察海桐皮汤提取膏对炎症模型小鼠的抗炎作用。结果:在镇痛方面,与模型组比较,工艺1组、工艺2组均可明显延长小鼠的热板痛阈值及扭体反应潜伏期(P<0.05),显著减少小鼠扭体的次数(P<0.001),且工艺1组的效果更明显(P<0.05);在抗炎方面,与空白组比较,模型组小鼠右后足肿胀度及肿胀率均明显增高(P<0.01),表明小鼠急性炎症造模成功。与模型组比较,工艺1组、工艺2组均能明显降低小鼠的足肿胀度和肿胀率(P<0.05),并降低小鼠的耳廓肿胀度,提升其肿胀抑制率(P<0.05),且工艺1组有效作用更显著(P<0.05)。与模型组相比,工艺1组可明显降低TNF-α、IL-1β的含量(P<0.01),与工艺2组相比,工艺1组可明显降低IL-1β含量(P<0.05)。结论:两种不同工艺制备的海桐皮汤提取膏经皮给药后对小鼠具有较好镇痛、抗炎作用,其作用机制可能与减少体内TNF-α、IL-1β、IL-6等促炎性细胞因子表达有关,且工艺1组抗炎镇痛效果优于工艺2组,可能与中药挥发油的单独提取、储存保证了挥发油较长时效的药理活性有关。