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Effects on Growth Performance and Feed Digestibility of Hainan Black Pigs by Adding Lysine in Low Protein Diet 被引量:4
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作者 Wei Limin Liu Quanwei +5 位作者 Sun Ruiping Zheng Xinli Wang Feng Tan Shuyi Huang Lili Xing Manping 《Animal Husbandry and Feed Science》 CAS 2018年第2期109-112,共4页
[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of ... [Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of (46.34±3.06) kg were randomly divided into three groups: con- trol group (protein level 15%, lysine level 0.83%), experimental grnup 1 (protein level 13%, 0.83%), and experimental group II (protein level 13%, lysine level 1.03), each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group (P〉0.05). The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly (P〈0.05), which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant (P〉0.05). [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig. 展开更多
关键词 Low prolein diets LYSINE hainan black pigs Growth performances Feed digestibility
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Polymorphic Analysis of TLR2 Gene in Native Hainan Pig Breeds Based on Sequencing Technology
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作者 Zhang Yan Liu Hailong +1 位作者 Wang Wenxiu Huang Lili 《Animal Husbandry and Feed Science》 CAS 2019年第5期135-139,共5页
[Objective]The paper was to understand the polymorphism of TLR2 gene in native Hainan pig breeds.[Method]TLR2 gene were cloned from blood samples of Wuzhishan pigs,Lingao pigs and Tunchang pigs by PCR and sequenced.[R... [Objective]The paper was to understand the polymorphism of TLR2 gene in native Hainan pig breeds.[Method]TLR2 gene were cloned from blood samples of Wuzhishan pigs,Lingao pigs and Tunchang pigs by PCR and sequenced.[Result]The DNA sequence of TLR2 gene in native Hainan pig breeds was 2649 bp and its CDS was 2358 bp.The intra-specific alignment of TLR2 gene sequences of the three pigs showed that there were seven nucleotide polymorphisms in Wuzhishan pigs(two of which located in the coding region),five nucleotide polymorphisms in Tunchang pigs(all of which were outside the coding region)and four nucleotide polymorphisms in Lingao pigs(one of which was located in the coding region).The results of inter-specific comparison showed that there were 12 nucleotide polymorphisms in three pig breeds,three of polymorphisms were missense mutations,resulting in changes in amino acids.The results of homologous analysis showed that the TLR2 gene sequences of the three pig breeds were highly conservative,with the homology ranging from 99.6% to 99.9%.Prediction and analysis of protein structure showed that AG mutation at 876 and 1454 sites of TLR2 gene in native Hainan pigs caused changes in secondary and tertiary structure of the protein,suggesting there might be possible changes in the function of TLR2.[Conclusion]The study provided reference for further research on the relationship between polymorphism of TLR2 gene and epidemic susceptibility of pigs. 展开更多
关键词 Native hainan pig TLR2 Gene polymorphism Gene sequencing
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