Objective The investigate the effect of polypeptide isolated from Chlamys Farreri (PCF) on Hela cells damaged by ultraviolet(UV). Methods The suspension of Hela epithelia cells (5 × 10^8/mL ) cultured for 24 ...Objective The investigate the effect of polypeptide isolated from Chlamys Farreri (PCF) on Hela cells damaged by ultraviolet(UV). Methods The suspension of Hela epithelia cells (5 × 10^8/mL ) cultured for 24 hours were prepared and 1 mL of it was put into each hole of 24-hole plates. Some Hela cells were randomly divided into six groups: control group (C), modal group(M), 5 g/L PCF group, 10 g/L PCF group, 20 g/L PC Fgroup and 10 g/L Vitamin-C (Vc) group. After incubation with RPMI-1640, PCF, Vitamin C, respectively, for 10minutes, the cells were exposed to UVA ( irradiation intensity: 3650 μ/cm^2 ), with the exception of the mice in C group. Some other Hela cells were divided into another six groups, they were treated almost the same as above, but irradiated by UVB ( irradiation intensity: 7.15 ×10^-5 J/cm^2). The intracellular free calcium (Ca^2+ ), apoptosis and cell death rate were measured by flow cytometry (FCM). Enzyme activities and malondialdehyde (MDA) were determined by biochemical assays. Results The apoptosis and cell death rate were decreased and the contents of intracellular Ca^2+ and activities of succinate dehydrogenase (SDH) as well as the activities of GSH-px, SOD,CAT increased and the MDA decreased by the action of PCF. Conclusion It is suggested that PCF could protect the cells from the damage caused by UVA and UVB by enhancing the activities of antioxidant enzymes.展开更多
文摘Objective The investigate the effect of polypeptide isolated from Chlamys Farreri (PCF) on Hela cells damaged by ultraviolet(UV). Methods The suspension of Hela epithelia cells (5 × 10^8/mL ) cultured for 24 hours were prepared and 1 mL of it was put into each hole of 24-hole plates. Some Hela cells were randomly divided into six groups: control group (C), modal group(M), 5 g/L PCF group, 10 g/L PCF group, 20 g/L PC Fgroup and 10 g/L Vitamin-C (Vc) group. After incubation with RPMI-1640, PCF, Vitamin C, respectively, for 10minutes, the cells were exposed to UVA ( irradiation intensity: 3650 μ/cm^2 ), with the exception of the mice in C group. Some other Hela cells were divided into another six groups, they were treated almost the same as above, but irradiated by UVB ( irradiation intensity: 7.15 ×10^-5 J/cm^2). The intracellular free calcium (Ca^2+ ), apoptosis and cell death rate were measured by flow cytometry (FCM). Enzyme activities and malondialdehyde (MDA) were determined by biochemical assays. Results The apoptosis and cell death rate were decreased and the contents of intracellular Ca^2+ and activities of succinate dehydrogenase (SDH) as well as the activities of GSH-px, SOD,CAT increased and the MDA decreased by the action of PCF. Conclusion It is suggested that PCF could protect the cells from the damage caused by UVA and UVB by enhancing the activities of antioxidant enzymes.
