The establishment of nucleus herds (NHs) of Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection...The establishment of nucleus herds (NHs) of Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection (MAS) is being utilized to identify individuals carrying favorable alleles of genes associated with production traits and screen out genetic defects (GD) for breeding purposes. Porcine Stress Syndrome (PSS) caused by a mutation in Halothane (HAL) gene is a GD frequently found in commercial breeds that when expressed, causes pale, soft, exudative (PSE) meat. PSE is inferior quality meat undesirable in the market causing economic losses to the swine industry. Thus, this study was conducted to screen the HAL gene through mutagenically separated-polymerase chain reaction (MSPCR) in selected NP herds and assessed its repeatability in local breeds. Results showed that out of 577 screened individuals, 543 (94.11%) were normal (NN), 0 (0%) were homozygous mutant (nn) and 34 (5.89%) were heterozygous carriers (Nn). Therefore, the optimized PSS screening protocol using MSPCR is also applicable to local breeds. As such, the availability of genetic tests for PSS could be useful in improving the Philippine NPs breeding selection and inhibiting or eliminating PSS mutant incidence within its nucleus herd.展开更多
[Objective] The aim was to investigate the distribution of the halothane （Haln） gene in breeding pig group in Beijing area.[Method] PCR-RFLP method was used to determine Haln genotype of 609 breeding pigs from boar ...[Objective] The aim was to investigate the distribution of the halothane （Haln） gene in breeding pig group in Beijing area.[Method] PCR-RFLP method was used to determine Haln genotype of 609 breeding pigs from boar stations and pig breeding farms in Beijing.[Result] The Haln gene frequencies of Large White,Landrace,Duroc and Pietrain pigs of the boar stations were 0,17.11%,5.56% and 18.75%,respectively; and the Haln gene frequencies of Large White,Landrace and Duroc pigs of pig breeding farms were 4.52%,11.68% and 23.33%,respectively.[Conclusion] The result provided a basis for the rational use of Haln gene.展开更多
A simple preparation using the ear tissue for PCR amplification was established for diagnosis of genotypes for halothane in 181 sows.3 halothane heterozygous pigs were detected.The behaviors of the sows that have diff...A simple preparation using the ear tissue for PCR amplification was established for diagnosis of genotypes for halothane in 181 sows.3 halothane heterozygous pigs were detected.The behaviors of the sows that have different genotypes were observed.The heterozygous sows expressed seem more behavioral stereotypies than halothane resistant.But there is no difference in two genotypes.The behaviour directed trough in heterozygous sows is higher than halothane resistant.展开更多
文摘The establishment of nucleus herds (NHs) of Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection (MAS) is being utilized to identify individuals carrying favorable alleles of genes associated with production traits and screen out genetic defects (GD) for breeding purposes. Porcine Stress Syndrome (PSS) caused by a mutation in Halothane (HAL) gene is a GD frequently found in commercial breeds that when expressed, causes pale, soft, exudative (PSE) meat. PSE is inferior quality meat undesirable in the market causing economic losses to the swine industry. Thus, this study was conducted to screen the HAL gene through mutagenically separated-polymerase chain reaction (MSPCR) in selected NP herds and assessed its repeatability in local breeds. Results showed that out of 577 screened individuals, 543 (94.11%) were normal (NN), 0 (0%) were homozygous mutant (nn) and 34 (5.89%) were heterozygous carriers (Nn). Therefore, the optimized PSS screening protocol using MSPCR is also applicable to local breeds. As such, the availability of genetic tests for PSS could be useful in improving the Philippine NPs breeding selection and inhibiting or eliminating PSS mutant incidence within its nucleus herd.
基金Supported by Excellent Talents Training Funded Projects of Organization Department of CPC Beijing Municipal Committee(20071D0200100039)Swine Industry Technology~~
文摘[Objective] The aim was to investigate the distribution of the halothane （Haln） gene in breeding pig group in Beijing area.[Method] PCR-RFLP method was used to determine Haln genotype of 609 breeding pigs from boar stations and pig breeding farms in Beijing.[Result] The Haln gene frequencies of Large White,Landrace,Duroc and Pietrain pigs of the boar stations were 0,17.11%,5.56% and 18.75%,respectively; and the Haln gene frequencies of Large White,Landrace and Duroc pigs of pig breeding farms were 4.52%,11.68% and 23.33%,respectively.[Conclusion] The result provided a basis for the rational use of Haln gene.
文摘A simple preparation using the ear tissue for PCR amplification was established for diagnosis of genotypes for halothane in 181 sows.3 halothane heterozygous pigs were detected.The behaviors of the sows that have different genotypes were observed.The heterozygous sows expressed seem more behavioral stereotypies than halothane resistant.But there is no difference in two genotypes.The behaviour directed trough in heterozygous sows is higher than halothane resistant.
