[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron ( C. A. Mey. ). [Method] H. ammodendron (C. A. Mey. ) seeds were used as experimental ...[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron ( C. A. Mey. ). [Method] H. ammodendron (C. A. Mey. ) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [ Result] Sterilization of H. ammodendron ( C.A. Mey. ) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min. Seeds with diameter φ 〉 2.0 mm were used as explants and achieved relatively high surviv- al rate of aseptic seedlings, which reached above 65%. [ Conclusion] This study established a surface sterilization method for tissue culture of H. ammodendron ( C. A. Mey. ) Bunge seeds.展开更多
基金Supported by Open Project of Key Laboratory of Tarim Animal Husbandry Science and Technology from Xinjiang Production & Construction Crops(HS20901)
文摘[ Objective ] The aim was to select appropriate sterilization methods and explants for tissue culture of Haloxylon ammodendron ( C. A. Mey. ). [Method] H. ammodendron (C. A. Mey. ) seeds were used as experimental materials to investigate the sterilization results of different sterilization treatments and the effect of different seed sizes on the survival rate of aseptic seedlings. [ Result] Sterilization of H. ammodendron ( C.A. Mey. ) seeds achieved the best result by using 75% alcohol for 15 min and 0.1% mercuric chloride for 8 min. Seeds with diameter φ 〉 2.0 mm were used as explants and achieved relatively high surviv- al rate of aseptic seedlings, which reached above 65%. [ Conclusion] This study established a surface sterilization method for tissue culture of H. ammodendron ( C. A. Mey. ) Bunge seeds.