[Objectives]To explore the effects of Abrus cantoniensis Hance(ACH)extract on blood lipid indicators of laying hen fed with high energy and low protein diet.[Methods]Sixty 90-day-old laying hens were randomly divided ...[Objectives]To explore the effects of Abrus cantoniensis Hance(ACH)extract on blood lipid indicators of laying hen fed with high energy and low protein diet.[Methods]Sixty 90-day-old laying hens were randomly divided into five groups:the blank control group(basic diet),the model group(high-energy and low-protein diet,HELPD),the low-dose group(HELPD+0.5 g ACH extract per hen,LACH),and the medium-dose group(HELPD+1 g ACH extract per hen,MACH),high dose group(HELPD+2 g ACH extract per hen,HACH).The ACH extract was administrated by drinking water for 48 d.[Results]Different doses of ACH could improve the pathological changes induced by high energy and low protein.ACH extract had no significant effect on blood routine indicators of laying hens(P>0.05).The contents of total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in the model group were significant-ly higher than those in the control group(P<0.05),while the content of high density lipoprotein cholesterol(HDL-C)was significantly lower than that in the control group(P<0.05).There was no significant difference in blood lipid between LACH group and model group(P>0.05).In MACH and HACH groups,the contents of TC,TG and LDL-C were significantly lower than those in the model group(P<0.05),and the content of HDL-C was significantly higher than that in the model group(P<0.05).[Conclusions]The ACH extract can regulate theHELPD-induced dyslipidemia in laying hens.展开更多
Objective:Gut-derived serotonin strongly inhibits bone formation by inhibiting osteoblast proliferation.Our previous study demonstrated that the lignan-rich fraction prepared from Sambucus willimasii Hance,a folk herb...Objective:Gut-derived serotonin strongly inhibits bone formation by inhibiting osteoblast proliferation.Our previous study demonstrated that the lignan-rich fraction prepared from Sambucus willimasii Hance,a folk herbal medicine used to treat bone fractures and joint diseases in China,exerted bone-protective effects,and its actions were modulated by suppressing the synthesis of gut-derived serotonin via the inhibition of intestinal tryptophan hydroxylase 1(TPH-1).However,there is no direct evidence for the action of lignans on TPH-1.This study aimed to verify the direct action of lignans on the TPH-1 and its influence on serotonin synthesis and bone properties.Methods:Molecular docking and surface plasmon resonance were performed to determine the affinities of lignans to TPH-1.The cell viability and the protein activity and expression of TPH-1 were measured in RBL2H3 cells.The serum serotonin level and bone mineral density upon lignan treatment in ovariectomized mice were determined.Result:The lignans showed high binding scores and binding affinities to TPH-1,inhibited the activity and protein expression of TPH-1,suppressed the serum serotonin levels in ovariectomized mice as well as promoted bone mineral density.Conclusion:This is the first study to report that lignans are novel TPH-1 inhibitors and that these lignans could be potential agents for the management of serotonin-related diseases,including osteoporosis.展开更多
[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were ...[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were determined in accordance with the inspection method of the Chinese Pharmacopoeia(2015).[Results]The 10 batches of L.foenum-graecum medicinal materials from different origins and different collection times in Guangxi all had the same plant morphology and medicinal properties.The moisture content was 5.69%-10.51%,the total ash content was 5.50%-8.61%,the acid-insoluble ash content was 0.42%-0.78%,and the extract was 15.28%-20.75%.[Conclusions]This study is expected to provide a scientific basis for the development and utilization of L.foenum-graecum medicinal materials and the establishment of its quality standards.展开更多
[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 co...[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.展开更多
为深入探索老山芹种子休眠机理,以老山芹层积前后种子为研究对象,采用气相色谱-飞行时间质谱联用技术(gas chromatography-time of flight mass spectrometry,GC-TOF/MS)对其非层积种子(non-lamellarized seeds,NS)和层积后达到发芽状...为深入探索老山芹种子休眠机理,以老山芹层积前后种子为研究对象,采用气相色谱-飞行时间质谱联用技术(gas chromatography-time of flight mass spectrometry,GC-TOF/MS)对其非层积种子(non-lamellarized seeds,NS)和层积后达到发芽状态种子(germination seeds,GS)的差异代谢物进行分析。结果表明,共检测到995种代谢产物,NS和GS的代谢物存在明显分离,差异代谢物有126种,其中极显著差异代谢物75种,包括35种上调,40种下调。对极显著差异代谢物所在代谢通路分析表明,涉及10条关键代谢通路。和弦图分析表明,差异代谢物中脂质类化合物(含类脂分子)与苯类化合物呈极显著正相关;苯丙烷类化合物(含聚酮类化合物)与有机氧类化合物呈极显著负相关。以上研究结果为探索老山芹种子成熟后萌发提供了代谢组学理论基础,为更好地研究老山芹种子后成熟及休眠机制提供了依据和支撑。展开更多
基金Supported by Special Project of Technological Innovation of Hainan Provincial Research Institute(SQKY2022-0006)Key Task of the Institute of Animal Husbandry and Veterinary of Hainan Agricultural Academy(HNXM2024ZD01).
文摘[Objectives]To explore the effects of Abrus cantoniensis Hance(ACH)extract on blood lipid indicators of laying hen fed with high energy and low protein diet.[Methods]Sixty 90-day-old laying hens were randomly divided into five groups:the blank control group(basic diet),the model group(high-energy and low-protein diet,HELPD),the low-dose group(HELPD+0.5 g ACH extract per hen,LACH),and the medium-dose group(HELPD+1 g ACH extract per hen,MACH),high dose group(HELPD+2 g ACH extract per hen,HACH).The ACH extract was administrated by drinking water for 48 d.[Results]Different doses of ACH could improve the pathological changes induced by high energy and low protein.ACH extract had no significant effect on blood routine indicators of laying hens(P>0.05).The contents of total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)in the model group were significant-ly higher than those in the control group(P<0.05),while the content of high density lipoprotein cholesterol(HDL-C)was significantly lower than that in the control group(P<0.05).There was no significant difference in blood lipid between LACH group and model group(P>0.05).In MACH and HACH groups,the contents of TC,TG and LDL-C were significantly lower than those in the model group(P<0.05),and the content of HDL-C was significantly higher than that in the model group(P<0.05).[Conclusions]The ACH extract can regulate theHELPD-induced dyslipidemia in laying hens.
基金supported by the Natural Science Foundation of Guangdong Province(2021A1515010648)the National Natural Science Foundation of China(81903616)+1 种基金The Hong Kong Polytechnic University Start-up Funding(A0038607)The Mainland-Hong Kong Joint Funding Scheme(ITFMOST:MHX/002/20).
文摘Objective:Gut-derived serotonin strongly inhibits bone formation by inhibiting osteoblast proliferation.Our previous study demonstrated that the lignan-rich fraction prepared from Sambucus willimasii Hance,a folk herbal medicine used to treat bone fractures and joint diseases in China,exerted bone-protective effects,and its actions were modulated by suppressing the synthesis of gut-derived serotonin via the inhibition of intestinal tryptophan hydroxylase 1(TPH-1).However,there is no direct evidence for the action of lignans on TPH-1.This study aimed to verify the direct action of lignans on the TPH-1 and its influence on serotonin synthesis and bone properties.Methods:Molecular docking and surface plasmon resonance were performed to determine the affinities of lignans to TPH-1.The cell viability and the protein activity and expression of TPH-1 were measured in RBL2H3 cells.The serum serotonin level and bone mineral density upon lignan treatment in ovariectomized mice were determined.Result:The lignans showed high binding scores and binding affinities to TPH-1,inhibited the activity and protein expression of TPH-1,suppressed the serum serotonin levels in ovariectomized mice as well as promoted bone mineral density.Conclusion:This is the first study to report that lignans are novel TPH-1 inhibitors and that these lignans could be potential agents for the management of serotonin-related diseases,including osteoporosis.
基金Key Research and Development Program of Department of Science and Technology of Guangxi Zhuang Autonomous Region(AB19110003)Scientific Research Program of Guangxi International Zhuang Medical Hospital in 2020(GZ202001)+2 种基金Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2019(2019KY0341)Open Program of Key Laboratory of Zhuang and Yao Medicine(GXZYKF2020A-08)Youth Foundation Project of Guangxi University of Chinese Medicine(2019QN036).
文摘[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were determined in accordance with the inspection method of the Chinese Pharmacopoeia(2015).[Results]The 10 batches of L.foenum-graecum medicinal materials from different origins and different collection times in Guangxi all had the same plant morphology and medicinal properties.The moisture content was 5.69%-10.51%,the total ash content was 5.50%-8.61%,the acid-insoluble ash content was 0.42%-0.78%,and the extract was 15.28%-20.75%.[Conclusions]This study is expected to provide a scientific basis for the development and utilization of L.foenum-graecum medicinal materials and the establishment of its quality standards.
基金Scientific Research and Technological Development Program Project of Hezhou City(He Ke Ji 20047)Scientific Research Program of Guangxi International Zhuang Medicine Hospital in 2020(GZ202001)+1 种基金Preparation Quality Improvement Project of Traditional Chinese Medicine Zhuang and Yao Medicine Hospital of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GZZJ202013&GZZJ202015)Zhuang and Yao Medicine Research Laboratory of Guangxi International Zhuang Medicine Hospital.
文摘[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.
文摘为深入探索老山芹种子休眠机理,以老山芹层积前后种子为研究对象,采用气相色谱-飞行时间质谱联用技术(gas chromatography-time of flight mass spectrometry,GC-TOF/MS)对其非层积种子(non-lamellarized seeds,NS)和层积后达到发芽状态种子(germination seeds,GS)的差异代谢物进行分析。结果表明,共检测到995种代谢产物,NS和GS的代谢物存在明显分离,差异代谢物有126种,其中极显著差异代谢物75种,包括35种上调,40种下调。对极显著差异代谢物所在代谢通路分析表明,涉及10条关键代谢通路。和弦图分析表明,差异代谢物中脂质类化合物(含类脂分子)与苯类化合物呈极显著正相关;苯丙烷类化合物(含聚酮类化合物)与有机氧类化合物呈极显著负相关。以上研究结果为探索老山芹种子成熟后萌发提供了代谢组学理论基础,为更好地研究老山芹种子后成熟及休眠机制提供了依据和支撑。
