The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with para...The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling （TUNEL） system. Four anatropous ovules in each ventricle could be observed before flowering. With the developing of floral organ, the bulk of normal ovules enlarged in each ventricle, the mature embryo sac differentiated into nucellus, and the egg cell developed into zygote by double fertilization. A large number of starch grains were observed during pollen tube growth and double fertilization, which guaranteed basic nutrient supply in the normal development of ovules. Moreover, abortion phenomenon of runtish ovules emerged at the stages of mature embryo sac, double fertilization and zygote development. The abortion characteristics included deformity of ovule development, degradation of nucellus tissue, separation between funiculus and ovule, abnormality of four-nucleate embryo sac, as well as development interruption of mature embryo sac. TUNEL analysis proved that ovule abortion was programmed cell death.展开更多
To find out a low temperature storage method for the maintenance of Hanfu apple’s firmness,crispness and moisture content,three storage methods in refrigerator,large cellar,and small cellar were experimented.Tests an...To find out a low temperature storage method for the maintenance of Hanfu apple’s firmness,crispness and moisture content,three storage methods in refrigerator,large cellar,and small cellar were experimented.Tests and comparisons were conducted for parameters including maximum absolute deformation,elastic modulus,firmness in compression test,and weight loss rate using mechanical characteristics monitoring and regular weighing during and after storage.Five testing groups with different pretreatments were arranged for refrigerator tests.The results showed that lower weight loss rates were achieved in two cellars than in the refrigerator;and difference between the large and small cellars was insignificant.However,cellar storage was only feasible for a relatively short term.Refrigeration storage with humidification sealing treatment yielded the optimal result in maintaining firmness,crispness and moisture of Hanfu apples,showing advantage in long-term storage.展开更多
基金the Key Scientific and Technological Research Project of Shenyang City,China(1071154-3-00,F12-109-3-00)the China Agriculture Research System(CARS-28)
文摘The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling （TUNEL） system. Four anatropous ovules in each ventricle could be observed before flowering. With the developing of floral organ, the bulk of normal ovules enlarged in each ventricle, the mature embryo sac differentiated into nucellus, and the egg cell developed into zygote by double fertilization. A large number of starch grains were observed during pollen tube growth and double fertilization, which guaranteed basic nutrient supply in the normal development of ovules. Moreover, abortion phenomenon of runtish ovules emerged at the stages of mature embryo sac, double fertilization and zygote development. The abortion characteristics included deformity of ovule development, degradation of nucellus tissue, separation between funiculus and ovule, abnormality of four-nucleate embryo sac, as well as development interruption of mature embryo sac. TUNEL analysis proved that ovule abortion was programmed cell death.
文摘To find out a low temperature storage method for the maintenance of Hanfu apple’s firmness,crispness and moisture content,three storage methods in refrigerator,large cellar,and small cellar were experimented.Tests and comparisons were conducted for parameters including maximum absolute deformation,elastic modulus,firmness in compression test,and weight loss rate using mechanical characteristics monitoring and regular weighing during and after storage.Five testing groups with different pretreatments were arranged for refrigerator tests.The results showed that lower weight loss rates were achieved in two cellars than in the refrigerator;and difference between the large and small cellars was insignificant.However,cellar storage was only feasible for a relatively short term.Refrigeration storage with humidification sealing treatment yielded the optimal result in maintaining firmness,crispness and moisture of Hanfu apples,showing advantage in long-term storage.
