[ Objective] This study aimed to isolate and identify Vibrio ichthyoenteri strains from diseased Hapalogenys nitens. [ Method] In January 2013, dis- eased H. nitens juveniles from a maricultural farm in Luoyuanwan Bay...[ Objective] This study aimed to isolate and identify Vibrio ichthyoenteri strains from diseased Hapalogenys nitens. [ Method] In January 2013, dis- eased H. nitens juveniles from a maricultural farm in Luoyuanwan Bay of Fuzhou City were selected to investigate the incidence, clinical symptoms and pathogenic characteristics. Liver, spleen, kidney and gill of five diseased H. nitens juveniles were collected for bacterial isolation and pure culture to conduct morphological, physiological and biochemical assays, 16S rRNA gene sequencing and phylogenetic analysis. The susceptibility of five strains to 30 kinds of antimicrobial drugs was detected. [ Result] The death of H. nitens juveniles was caused by bacterial infections. The isolated strains fzlyw201301071 and fzlyw201301072 belonged to the same genus as Vibrio ichthyoenteri. Drug susceptibihty test suggested that there were no significant differences in drug sensitivity and resistance among different strains. [ Conclusion] This study provided theoretical basis for the prevention and control of diseases in aquaculture animals.展开更多
基金Supported by Program of Aquatic Animal Disease Monitoring in Fujian Province(2012)Program of Fishery Pest Control in Fuzhou City(2012)
文摘[ Objective] This study aimed to isolate and identify Vibrio ichthyoenteri strains from diseased Hapalogenys nitens. [ Method] In January 2013, dis- eased H. nitens juveniles from a maricultural farm in Luoyuanwan Bay of Fuzhou City were selected to investigate the incidence, clinical symptoms and pathogenic characteristics. Liver, spleen, kidney and gill of five diseased H. nitens juveniles were collected for bacterial isolation and pure culture to conduct morphological, physiological and biochemical assays, 16S rRNA gene sequencing and phylogenetic analysis. The susceptibility of five strains to 30 kinds of antimicrobial drugs was detected. [ Result] The death of H. nitens juveniles was caused by bacterial infections. The isolated strains fzlyw201301071 and fzlyw201301072 belonged to the same genus as Vibrio ichthyoenteri. Drug susceptibihty test suggested that there were no significant differences in drug sensitivity and resistance among different strains. [ Conclusion] This study provided theoretical basis for the prevention and control of diseases in aquaculture animals.
