[Objectives]This study was conducted to investigate the effects of dietary straw content on the growth and development of oriental migratory locusts(Locusta migratoria),as well as the effects of temperature and substr...[Objectives]This study was conducted to investigate the effects of dietary straw content on the growth and development of oriental migratory locusts(Locusta migratoria),as well as the effects of temperature and substrate on their egg hatching.[Methods]The eggs of oriental migratory locusts were hatched at 15,20,25,30,35,and 40℃in sawdust,soil and sawdust+soil conditions,respectively,to determine suitable environmental conditions for artificial hatching of oriental migratory locusts.Rice straw was added at 0%,20%,40%,60%,80%and 100%to the basic diet,and the obtained diets was feed to oriental migratory locusts at the 4^(th)instar,to investigate the effects on their growth and development.[Results]The eggs of oriental migratory locusts had a good hatching rate at 25-30℃,and sawdust was the best choice for their hatching substrate.Adding 40%of rice straw to the diet could ensure the normal growth and development of oriental migratory locusts while consuming as much rice straw as possible.[Conclusions]This study explored a technical solution for efficiently producing insect protein from discarded crop straw(treating waste with insects,and turning waste into treasure),providing support for breeding of oriental migratory locusts and locust disaster prevention.展开更多
[Objective]To improve the artificial propagation technique of Misgurnus anguillicaudatus,and to provide technical support for the industrialization development of M.anguillicaudatus in future.[Methods] The hatching ra...[Objective]To improve the artificial propagation technique of Misgurnus anguillicaudatus,and to provide technical support for the industrialization development of M.anguillicaudatus in future.[Methods] The hatching rates,survival rates,deformity rates and parent morality rates of M.anguillicaudatus were compared by ecological hatching method and traditional hatching method.[Results]The hatching rate by ecological hatching method was 32.60% higher than that by traditional hatching method,showing extremely significant differences.The survival rate of M.anguillicaudatus fingerlings by ecological hatching method was2.32% higher than that by traditional hatching method.And the deformity rates of M.anguillicaudatus fingerlings by traditional hatching method and ecological hatching method were 26.50% and 24.14%,respectively,indicating no significant differences.The morality rates of female parents of M.anguillicaudatus were89.72% and 5.91% by traditional hatching method and ecological hatching method,respectively.[Conclusions]Ecological hatching method effectively improved the hatching rate and fingerling survival rate of M.anguillicaudatus,and reduced the morality rate of female parents,which was helpful to save the production cost,met the requirements for large-scale production of M.anguillicaudatus,and had good application prospect.展开更多
Purpose: Impaired hatching is associated with implantation failure following in vitro fertilization (IVF). Thickening or hardening of the zona pellucida (ZP) has been proposed as a factor in this impairment. We examin...Purpose: Impaired hatching is associated with implantation failure following in vitro fertilization (IVF). Thickening or hardening of the zona pellucida (ZP) has been proposed as a factor in this impairment. We examined whether selective assisted hatching (AH) is beneficial with embryos having a thick ZP. Methods: This prospective, randomized controlled study was performed in the IVF unit of an obstetrics and gynecology department in a university-affiliated hospital. Only patients undergoing IVF and having a ZP thickness of ≥17 μm measured in all their embryos were included. In the intervention group, AH was applied to all embryos, before their transfer. In the control group, embryos were transferred without AH. Implantation, clinical pregnancy and live birth rates were the study endpoints. Results: Both study arms were comparable in most baseline parameters. The two groups did not differ in implantation rates (14.1% control vs. 8.92% intervention, odds ratio (OR) = 0.5974, 95% confidence interval (CI) 0.325 - 1.1), clinical pregnancy rates (36.7% vs. 25.8%, OR = 0.6025, 95% CI 0.274 - 1.325), or live birth rates (25% vs. 18.9%, OR = 0.7021, 95% CI 0.291 - 1.691). Conclusions: Selecting embryos for AH by their ZP thickness as a sole parameter was not found to be beneficial and to improve IVF outcome.展开更多
Objective: To determine whether laser-assisted hatching can improve clinical outcome of assisted reproductive techniques in both unselected patients and patients with advanced female age, with recurrent implantation f...Objective: To determine whether laser-assisted hatching can improve clinical outcome of assisted reproductive techniques in both unselected patients and patients with advanced female age, with recurrent implantation failure, or who are using frozen-thawed embryos. Study design: Prospective randomized study. Patients and methods: 179 consecutive women scheduled for intracytoplasmic sperm injection (ICSI) were randomised to either laser assisted hatching (LAH) or non LAH group. Patients were divided into 94 test and 85 control groups. On the day of embryo transfer, the zona pellucida of the selected embryos in the test group was thinned by using an infrared optical laser system, whereas in the control group they were left intact. Clinical pregnancy rates (CPR) and implantation rates (IR) were estimated. Result(s): Patients that underwent LAH (n = 94) had CPR, and IR of 35.1% and 50%, respectively. Patients that did not undergo LAH (n = 85) had lower CPR (28.2%, P = 0.324) and IR (33%, P = 230), with no statistically significant difference P Conclusion: The current study suggested that there is no need for the routine use of laser assisted hatching. However, there is evidence of laser assisted hatching benefit in selected展开更多
旨在揭示白羽肉鸡孵化性状的遗传基础。本试验以白羽肉鸡A、B两个公鸡群体为素材,测定A群体5个世代(556只)、B群体3个世代(398只)的40周龄种蛋受精率和孵化率,并利用55K SNP芯片对两个群体共954个健康个体进行基因分型。基于系谱和基因...旨在揭示白羽肉鸡孵化性状的遗传基础。本试验以白羽肉鸡A、B两个公鸡群体为素材,测定A群体5个世代(556只)、B群体3个世代(398只)的40周龄种蛋受精率和孵化率,并利用55K SNP芯片对两个群体共954个健康个体进行基因分型。基于系谱和基因型信息计算的A群体的受精率遗传力分别为0.21和0.14。利用混合线性模型对受精率和孵化率进行全基因组关联分析(genome-wide association study,GWAS)。全基因组关联分析共关联到12个显著SNPs,分布在1、9、11、12、20号染色体上,其中有9个SNPs与受精率显著关联,有3个SNPs与孵化率显著关联,对A群体受精率高、低组各4只公鸡睾丸组织进行转录组测序,实时荧光定量PCR验证两个差异表达基因。全基因组关联分析筛选出9个与受精率相关的候选基因COPG1、ADAMTS18、FABP2、CDH8、SLCO4A1、ATP13A3、NELL2、VEZT和IGHMBP2;3个与孵化率相关的候选基因TMEM、SCO1和MYH1A。转录组测序与荧光定量PCR验证了两个与受精率相关的候选基因HMGCLL1和COA6。本研究利用GWAS和转录组测序筛选到孵化性状的相关候选基因,为白羽肉鸡孵化性状的遗传改良提供了重要的分子标记。展开更多
基金Supported by National Innovation and Entrepreneurship Program for College Students(201910223022)Daqing"Jiebangguashuai"Science and Technology Key Project in the New Energy Field(2021BD05)。
文摘[Objectives]This study was conducted to investigate the effects of dietary straw content on the growth and development of oriental migratory locusts(Locusta migratoria),as well as the effects of temperature and substrate on their egg hatching.[Methods]The eggs of oriental migratory locusts were hatched at 15,20,25,30,35,and 40℃in sawdust,soil and sawdust+soil conditions,respectively,to determine suitable environmental conditions for artificial hatching of oriental migratory locusts.Rice straw was added at 0%,20%,40%,60%,80%and 100%to the basic diet,and the obtained diets was feed to oriental migratory locusts at the 4^(th)instar,to investigate the effects on their growth and development.[Results]The eggs of oriental migratory locusts had a good hatching rate at 25-30℃,and sawdust was the best choice for their hatching substrate.Adding 40%of rice straw to the diet could ensure the normal growth and development of oriental migratory locusts while consuming as much rice straw as possible.[Conclusions]This study explored a technical solution for efficiently producing insect protein from discarded crop straw(treating waste with insects,and turning waste into treasure),providing support for breeding of oriental migratory locusts and locust disaster prevention.
基金Supported by the Basic Scientific Research Project of Henan Province
文摘[Objective]To improve the artificial propagation technique of Misgurnus anguillicaudatus,and to provide technical support for the industrialization development of M.anguillicaudatus in future.[Methods] The hatching rates,survival rates,deformity rates and parent morality rates of M.anguillicaudatus were compared by ecological hatching method and traditional hatching method.[Results]The hatching rate by ecological hatching method was 32.60% higher than that by traditional hatching method,showing extremely significant differences.The survival rate of M.anguillicaudatus fingerlings by ecological hatching method was2.32% higher than that by traditional hatching method.And the deformity rates of M.anguillicaudatus fingerlings by traditional hatching method and ecological hatching method were 26.50% and 24.14%,respectively,indicating no significant differences.The morality rates of female parents of M.anguillicaudatus were89.72% and 5.91% by traditional hatching method and ecological hatching method,respectively.[Conclusions]Ecological hatching method effectively improved the hatching rate and fingerling survival rate of M.anguillicaudatus,and reduced the morality rate of female parents,which was helpful to save the production cost,met the requirements for large-scale production of M.anguillicaudatus,and had good application prospect.
文摘Purpose: Impaired hatching is associated with implantation failure following in vitro fertilization (IVF). Thickening or hardening of the zona pellucida (ZP) has been proposed as a factor in this impairment. We examined whether selective assisted hatching (AH) is beneficial with embryos having a thick ZP. Methods: This prospective, randomized controlled study was performed in the IVF unit of an obstetrics and gynecology department in a university-affiliated hospital. Only patients undergoing IVF and having a ZP thickness of ≥17 μm measured in all their embryos were included. In the intervention group, AH was applied to all embryos, before their transfer. In the control group, embryos were transferred without AH. Implantation, clinical pregnancy and live birth rates were the study endpoints. Results: Both study arms were comparable in most baseline parameters. The two groups did not differ in implantation rates (14.1% control vs. 8.92% intervention, odds ratio (OR) = 0.5974, 95% confidence interval (CI) 0.325 - 1.1), clinical pregnancy rates (36.7% vs. 25.8%, OR = 0.6025, 95% CI 0.274 - 1.325), or live birth rates (25% vs. 18.9%, OR = 0.7021, 95% CI 0.291 - 1.691). Conclusions: Selecting embryos for AH by their ZP thickness as a sole parameter was not found to be beneficial and to improve IVF outcome.
文摘Objective: To determine whether laser-assisted hatching can improve clinical outcome of assisted reproductive techniques in both unselected patients and patients with advanced female age, with recurrent implantation failure, or who are using frozen-thawed embryos. Study design: Prospective randomized study. Patients and methods: 179 consecutive women scheduled for intracytoplasmic sperm injection (ICSI) were randomised to either laser assisted hatching (LAH) or non LAH group. Patients were divided into 94 test and 85 control groups. On the day of embryo transfer, the zona pellucida of the selected embryos in the test group was thinned by using an infrared optical laser system, whereas in the control group they were left intact. Clinical pregnancy rates (CPR) and implantation rates (IR) were estimated. Result(s): Patients that underwent LAH (n = 94) had CPR, and IR of 35.1% and 50%, respectively. Patients that did not undergo LAH (n = 85) had lower CPR (28.2%, P = 0.324) and IR (33%, P = 230), with no statistically significant difference P Conclusion: The current study suggested that there is no need for the routine use of laser assisted hatching. However, there is evidence of laser assisted hatching benefit in selected
文摘旨在揭示白羽肉鸡孵化性状的遗传基础。本试验以白羽肉鸡A、B两个公鸡群体为素材,测定A群体5个世代(556只)、B群体3个世代(398只)的40周龄种蛋受精率和孵化率,并利用55K SNP芯片对两个群体共954个健康个体进行基因分型。基于系谱和基因型信息计算的A群体的受精率遗传力分别为0.21和0.14。利用混合线性模型对受精率和孵化率进行全基因组关联分析(genome-wide association study,GWAS)。全基因组关联分析共关联到12个显著SNPs,分布在1、9、11、12、20号染色体上,其中有9个SNPs与受精率显著关联,有3个SNPs与孵化率显著关联,对A群体受精率高、低组各4只公鸡睾丸组织进行转录组测序,实时荧光定量PCR验证两个差异表达基因。全基因组关联分析筛选出9个与受精率相关的候选基因COPG1、ADAMTS18、FABP2、CDH8、SLCO4A1、ATP13A3、NELL2、VEZT和IGHMBP2;3个与孵化率相关的候选基因TMEM、SCO1和MYH1A。转录组测序与荧光定量PCR验证了两个与受精率相关的候选基因HMGCLL1和COA6。本研究利用GWAS和转录组测序筛选到孵化性状的相关候选基因,为白羽肉鸡孵化性状的遗传改良提供了重要的分子标记。
