Objectives:This paper aims to investigate the polyphenol metabolite variation and bioactivities of different-colored hawthorn berries(Crataegus pinnatifida).Materials and Methods:Comparative metabolomics analysis betw...Objectives:This paper aims to investigate the polyphenol metabolite variation and bioactivities of different-colored hawthorn berries(Crataegus pinnatifida).Materials and Methods:Comparative metabolomics analysis between peel and flesh of yellow hawthorn'Jinruyi'(JRY)and red-skinned ones was carried out by UPLC-MS/MS.Antioxidant activities andα-glucosidase inhibition capacity were also tested among different colored hawthorn samples.Results:A total of 453 polyphenols was characterized,among which phenolic acids and flavonoids were abundant,and were closely relevant to the antioxidant capacity of hawthorn fruits.Polyphenol profile showed accession-specific accumulation in peel or flesh of different colored hawthorn.The unique yellow hawthorn was found to have few anthocyanins but showed enhanced flux to synthesize flavones and flavonols,especially flavone C-glycosides and acylated flavonol glycosides.The specific acylation decoration included acetylation,p-coumaroylation,and malonylation.In addition,yellow hawthorn showed excellentα-glucosidase inhibitory effect,which might be associated with the high concentration of 8 polyphenols including 5 phenolic acids,2 flavone C-glycosides,and an acylated flavonol glycoside,namely,quercetin-7-O-(6ʹʹ-malonyl)glucoside.Such acylated flavonol showed the strongest correlation with the inhibition effect of hawthorn fruits onα-glucosidase,and was predicted to have the lowest binding energy with the enzyme according to molecular docking analysis,indicating its great potential as a strongα-glucosidase inhibitor and an important antidiabetic ingredient in yellow hawthorn.Conclusions:The acylated flavonol glycosides and C-glycosyl flavones might be chemotaxonomic markers differentiating varieties and bioactivities of yellow hawthorn from the traditional red-skinned ones.These findings complement the existing knowledge on the metabolite composition and nutritional properties of hawthorn fruits.展开更多
基金supported by the Key Research and Development Program of Zhejiang Province(2021C02001)the Natural Science Foundation of Shandong Province(No.ZR2023QC228)+1 种基金the Serving Local Economic Development Project of Shandong(Linyi)Institute of Modern Agriculture,Zhejiang University(No.ZDNY-2021-FWLY01004)the Fundamental Research Funds for the Central Universities(No.226-2022-00215),China.
文摘Objectives:This paper aims to investigate the polyphenol metabolite variation and bioactivities of different-colored hawthorn berries(Crataegus pinnatifida).Materials and Methods:Comparative metabolomics analysis between peel and flesh of yellow hawthorn'Jinruyi'(JRY)and red-skinned ones was carried out by UPLC-MS/MS.Antioxidant activities andα-glucosidase inhibition capacity were also tested among different colored hawthorn samples.Results:A total of 453 polyphenols was characterized,among which phenolic acids and flavonoids were abundant,and were closely relevant to the antioxidant capacity of hawthorn fruits.Polyphenol profile showed accession-specific accumulation in peel or flesh of different colored hawthorn.The unique yellow hawthorn was found to have few anthocyanins but showed enhanced flux to synthesize flavones and flavonols,especially flavone C-glycosides and acylated flavonol glycosides.The specific acylation decoration included acetylation,p-coumaroylation,and malonylation.In addition,yellow hawthorn showed excellentα-glucosidase inhibitory effect,which might be associated with the high concentration of 8 polyphenols including 5 phenolic acids,2 flavone C-glycosides,and an acylated flavonol glycoside,namely,quercetin-7-O-(6ʹʹ-malonyl)glucoside.Such acylated flavonol showed the strongest correlation with the inhibition effect of hawthorn fruits onα-glucosidase,and was predicted to have the lowest binding energy with the enzyme according to molecular docking analysis,indicating its great potential as a strongα-glucosidase inhibitor and an important antidiabetic ingredient in yellow hawthorn.Conclusions:The acylated flavonol glycosides and C-glycosyl flavones might be chemotaxonomic markers differentiating varieties and bioactivities of yellow hawthorn from the traditional red-skinned ones.These findings complement the existing knowledge on the metabolite composition and nutritional properties of hawthorn fruits.
