A simple and sensitive high performance liquid chromatography-chemical vapour generation-atom fluorescent spectrometry (HPLC-CVG-AFS) method was developed and validated for simultaneous determination mercury species i...A simple and sensitive high performance liquid chromatography-chemical vapour generation-atom fluorescent spectrometry (HPLC-CVG-AFS) method was developed and validated for simultaneous determination mercury species in Su-He-Xiang-Wan (SHXW) and in tissues of rats, respectively. The species of mercury were separated by a Venusil MP-C18 (5 μm, 150 mm×4.6 mm) column with the optimized mobile phase containing 5% (w/v) acetonitrile, 0.01 mol/L L-cysteine and 0.06 moL/L ammonium acetate. The tissues of rats were freeze-dried after giving the medicine for 10 d, and then added into the solution containing 10% (w/v) HCl, 1% (w/v) sulfocarbamide and 0.15% (w/v) KCl for increasing extraction rate. The resolutions of Hg2+ , MeHg and EtHg were 1.5 and 2.9, respectively. The detection limits of Hg2+ , MeHg and EtHg were 2.0, 1.0 and 0.9ng/mL, respectively. The relative standard deviation (RSD) of inter- and intra-day precisions ranged from 1.56% to 2.86%. The recovery rates of three different adding level were 87%-101% (n=6), and the RSDs were smaller than 8.2%. The results show that no MeHg and EtHg were detected in rat tissues. Only soluble mercury (Hg2+ ) was determined for the mercury species of SHXW in rat tissues.展开更多
基金Projects(20875104, 21075138) supported by the National Natural Science Foundation of China
文摘A simple and sensitive high performance liquid chromatography-chemical vapour generation-atom fluorescent spectrometry (HPLC-CVG-AFS) method was developed and validated for simultaneous determination mercury species in Su-He-Xiang-Wan (SHXW) and in tissues of rats, respectively. The species of mercury were separated by a Venusil MP-C18 (5 μm, 150 mm×4.6 mm) column with the optimized mobile phase containing 5% (w/v) acetonitrile, 0.01 mol/L L-cysteine and 0.06 moL/L ammonium acetate. The tissues of rats were freeze-dried after giving the medicine for 10 d, and then added into the solution containing 10% (w/v) HCl, 1% (w/v) sulfocarbamide and 0.15% (w/v) KCl for increasing extraction rate. The resolutions of Hg2+ , MeHg and EtHg were 1.5 and 2.9, respectively. The detection limits of Hg2+ , MeHg and EtHg were 2.0, 1.0 and 0.9ng/mL, respectively. The relative standard deviation (RSD) of inter- and intra-day precisions ranged from 1.56% to 2.86%. The recovery rates of three different adding level were 87%-101% (n=6), and the RSDs were smaller than 8.2%. The results show that no MeHg and EtHg were detected in rat tissues. Only soluble mercury (Hg2+ ) was determined for the mercury species of SHXW in rat tissues.
