Efficacy of He-Ne Laser in Combination with Topical Clindamycin in the Treatment of Acne Vulgaris (AV)

Objective: To evaluate the efficacy of He-Ne laser in combination with topical clindamycin in the treatment of AV at 108 Military Central Hospital from Oct 2015 to Aug 2016. Subjects and Methods: 61 patients with AV were divided into 2 groups: Group 1: 31 patients were treated with He-Ne laser in combination with topical Clindamycin, Group 2: 30 patients were only treated with topical clindamycin. Laser HE-NE was applied 2 times/week for 6 weeks. Result: After 3 months of treatment Group 1: very good—51.6%, good—48.4%. Group 2: very good—30%, good—50% and moderate—20%. Conclusion: The combination of He-Ne laser with topical clindamycin shows more rapid clinical improvement compared to topical clindamycin alone in the treatment of mild and moderate AV.

Effects of He-Ne laser irradiation on chronic atrophic gastritis in rats

AIM: To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats.METHODS: Sixty-three male adult Wistar rats were randomly divided into five groups including normal control group, model control group and three different dosages He-Ne laser groups. The chronic atrophic gastritis (CAG)model in rats was made by pouring medicine which was a kind of mixed liquor including 2% sodium salicylate and 30% alcohol down the throat for 8 wk to stimulate rat gastric mucosa, combining with irregular fasting and compulsive sporting as pathogenic factors; 3.36, 4.80, and 6.24J/cm2doses of He-Ne laser were used, respectively for three different treatment groups, once a day for 20 d. The pH value of diluted gastric acid was determined by acidimeter,the histopathological changes such as the inflammatory degrees in gastric mucosa, the morphology and structure of parietal cells were observed, and the thickness of mucosa was measured by micrometer under optical microscope.RESULTS: In model control group, the secretion of gastric acid was little, pathologic morphological changes in gastric mucosa such as thinner mucous, atrophic glands, notable inflammatory infiltration were found. After 3.36 J/cm2 dose of He-Ne laser treatment for 20 d, the secretion of gastric acid was increased (P＜0.05), the thickness of gastric mucosa was significantly thicker than that in model control group (P＜0.01), the gastric mucosal inflammation cells were decreased (P＜0.05). Morphology, structure and volume of the parietal cells all recuperated or were closed to normal.CONCLUSION: 3.36J/cm2 dose of He-Ne laser has a significant effect on CAG in rats.

Effects of the He-Ne laser with various power densities on the growth of cultured fibroblasts in hypertrophic scar

Objective To explore the relationship between power density of He -Ne laser with the growth of fibroblasts in hypertroph ic scar (HS).Methods The cultured fibroblasts in HS were i rradiated with He -Ne laser(Wavelenth 632.8nm),various power densities such as50mW/cm 2 ,100mW/cm 2 and 150mW/cm 2 were respectively adopted once a day for 10minutes.After 1,3and 5times o f He -Ne laser for dose rate irradiation separatedly ,the cell count and cell circle analysis were counted and examined by trypa n blue staining and flow cytometry .Results The amount of cell after 1times of 50mW /cm 2 laser irradiation was markedly more than teh control(P<0.01).The amount of cells after 5times of 100mW/cm 2 ,3and 5times of 150mW/cm 2 laser irradiation was less than the controls(P <0.05).The result of cell circle analysis was corresponded with that of the cell count.Conclusion Both stimualtion and inhibition of He -Ne laser on the growth of scar fibroblasts can be obtained with vario us power densities.Power density of He -Ne laser associates with the effects on the growth of scar fibroblasts.

The influence of He-Ne laser on scar formation after trabeculectomy in rabbits

AIM:To investigate the influence of He-Ne lasers on scar formation in the filtration canal after trabeculectomy in a rabbit model,as well as to explore the mechanisms for preventing scar formation when using He-Ne lasers in vivo. METHODS:Experiment 1:Four groups were established （four eyes in each group）.In 12 eyes,the upper nasal limbus area next to the upper rectus muscle received 10 minutes of He-Ne laser irradiation（100,150,200mW/cm2;60,90, 120J/cm2）every day for three days.Four eyes served as controls.Twenty-four hours after the final irradiation,the rabbits were sacrificed and the irradiated tissue was excised, fixed with paraformaldehyde and tested for proliferating cell nuclear antigen（PCNA）,connective tissue growth factor （CTGF）and apoptosis（TUNEL）.Experiment 2:Forty-two rabbits were randomly divided into two groups and standard trabeculectomy was performed in the right eyes either after 200mW/cm2 He-Ne laser irradiation or not in the filtration area.The expression of PCNA and CTGF,apoptosis and collagen density in the filtration area were tested on the 7th, 14thand 28thday after surgery. RESULTS:Experiment 1:There were no more PCNA and CTGF positive cells in the He-Ne irradiation group than in the control group.No apoptotic cells were found in either group. Experiment 2:The expression of PCNA and CTGF was lower in the He-Ne irradiation group than in the control group on the 7thand 14thday after trabeculectomy surgery（P【0.05）; no apoptotic cells were detected in either group.Collagen density was significantly lower in the He-Ne irradiation group than in the control group on the 14thand 28thday after surgery（P【0.05）. CONCLUSION:Pretreating the filtration area with 200mW/cm2 （120J/cm2）of He-Ne laser irradiation may be helpful in preventing scar formation after trabeculectomy,possibly due to the downregulation of the expression of PCNA,CTGF and collagen synthesis in fibroblasts.

The Effects of He-Ne Laser and Enhanced Ultraviolet-B Radiation on Proliferating-Cell Nuclear Antigen in Wheat Seedlings

The level of ultraviolet-B (UV-B) radiation on the Earth’s surface has increased due to depletion of the ozone layer. Here, we explored the effects of continuous wave He-Ne laser irradiation (632 nm, 5 mW·mm-2, 2 min·d-1) on proliferating-cell nuclear antigen (PCNA) damage repair function of wheat seedlings exposed to enhanced UV-B radiation (10.08 kJ&middotm-2·d-1) at the early growth stages. Wheat seedlings were irradiated with enhanced UV-B, He-Ne laser treatment or a combination of the two. We explored the transcripts of PCNA in each treatment group using RT-PCR. In addition, total proteins were extracted from the 7-day-old wheat leaves, analyzed by SDS-PAGE and identified by western blot. The results showed that the transcription of PCNA was weakened following UV-B radiation compared to the control. However, when seedlings were subjected to elevated UV-B-damaging radiation followed by He-Ne laser irradiation, the expression of PCNA was signifi-cantly higher than UV-B radiation alone. These results suggest that He-Ne laser has an active role in repairing the UV-B damaging effects. In order to further investigate the function of PCNA, dynamic arrangements of PCNA in wheat root-tip cells were observed with confocal laser scanning microscopy (CLSM). The PCNA was marked fluorescent dimming and strength weakened in en-hanced UV-B radiation (UV-B) compared with the control group (CK) during processing. It shows that PCNA may be involved in the separation of chromosomes.

Inhibitory effects of He-Ne laser repeated irradiation on collagen synthesis in hypertrophic scar-derived fibroblasts in culture

Objective To explore the inhibitory effects of He-Ne laser repeated irradiation on the collagen synthesis of cultured scar fibroblasts. Method Cultured fibroblasts derived from hypertrophic scars(HS) were irradiated with He-Ne laser for 30 minutes at various power densities(10,50,100 and 150 mW/cm2),once a day for 3 consecutive days.In 24 hours after repeated irradiation collagen production and type I procollagen mRNA level of fibroblasts were measured with the incorporation of 3H proline and blot hybridization techniques respectively.Results Collagen synthesis and type I procollagen mRNA level remained unchanged when the laser was irradiated at the power density of 10 mW/cm2 or 50 mW/cm2.Compared with control,collagen synthesis and type I procollagne mRNA level were significantly decreases at the power density of 100 mW/cm2 or 150mW/cm2(P< 0.05).Type I procollagen mRNA level at the power density of 150 mW/cm2 was lower than that at the 100 mW/cm2 (P< 0.05).Conclusion Repeated He-Ne laser irradiation at the power density of 100 mW/cm2 or 150 mW/cm2 can suppress collagen synthesis of cultured fibroblasts in HS.The cause of suppression may be associated with down regulation of type I procollagen mRNA expression.

Protecting effect of He-Ne laser on winter wheat from UV-B radiation damage by analyzing proteomic changes in leaves

The ultraviolet-B (UV-B) radiation can affect gene expression of plant in growth and development. Winter wheat (Triticum aestivum), as a kind of economic crop cultured in the Northern China, is also damaged by present-day ultraviolet-B (UV-B) radiation. It was reported that He-Ne lasers could alleviate cell damage caused by UV-B radiation in plants. To get the proteomic changes of wheat alleviated by He-Ne lasers, we studied protein expression profiles of winter wheat (Jin Mai NO.79) leaves by running 2-DE (two-dimensional gel electrophoresis), which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, in which they were confirmed to be winter wheat proteins. The results showed: 1) the proteomic changes between the ultraviolet-B radiation group and normal light group were significantly different on sixth day;2) The expression of some proteins such as catalase, DNA polymerase and reduced glutathione declines at the ultraviolet-B radiation group and increases at the UV-B + He-Ne laser group. This indicates UV-B could regulate genes encoding proteins in Winter wheat leaves and affect the growth of the Winter wheat. He-Ne lasers treatment could significantly alleviate UV-B-induced damage in wheat.

Enhancement of Human Blood Storage Period by Irradiation of Low Level He-Ne Laser

The aim of the present work is to investigate the effect of the He-Ne laser irradiation on the whole human blood (HB) in order to enhance the conditions of conservation. The HB was irradiated by He-Ne laser;(λ = 632 nm, continuous wave, power 30 mW, 2 mm diameter beam spot), electrical properties and complete blood count CBC were measured at three doses (0.0287, 0.0563 and 0.198 J/cm3) to the relevant best exposure dose during storage periods 9, 24, 30, 35 & 50 days. The irradiation process with the selected dose was performed by the exposure of the laser beam on the blood sample flow through narrow tube of cross section area, 0.0831 cm2. Blood dielectric parameters, (electric conductivity, dielectric constant, dielectric loss and dipole moment) and CBC, (red blood cell, white blood cell, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, and mean cell or corpuscular hemoglobin in concentration) were measured. The obtained results were compared with that of the control and showed that the best irradiation exposure dose suitable for increasing the time of blood storage with minimum changes in properties is 0.198 J/cm3 and storage period of about 50 days. The present study revealed that irradiation by He-Ne laser could be considered a good means to improve the conservation conditions of human blood.

THE PHOTOPOLYMERIZATION KINETICS OF PHOTOSENSITIZED ACRYLAMIDE SYSTEM INDUCED BY He-Ne LASER

The kinetics of the photopolymerization of sensitized acrylamide (AM) system induced by He-Ne laser has been investigated. Using methylene blue (MB)-triethanolamine (TEOA) as the photosensitive system, the photopolymerization followed a nonsteady-state kinetic scheme in the initial period of polymerization (the monomer conversion C%【2%) and then followed a steady-state kinetic scheme (5% 】C%】2%). Accirding to the steady-state hypothesis, the mechanism of photopolymerization was proposed. The deduced kinetic equation of the photopolymerization of AM is in good coincidence with the experimental results.

The Photopolymerization Kinetics of Acryl amide Sensitized with Toluidine Blue-Alcoholamine Induced by He-Ne Laser

Toluidine blue(TB) is a good sensitizer for the polymerization of acrylnmide(AM) induced by He-Ne laser. With TB as a sensitizer and various alcoholamine as donors, the photoredox of TB and the polymerization kinetics of AM induced by He-Ne laser were investigated. It was found that either for the photoredox of TB or for the polymerization of AM, the activity of alcoholamine increases in the order of EOA【DEOA 【TEOA in TB-alcoholamine sensitized system. The overall apparent kinetic equations of AM polymerization at 30℃ have been determined. In addition, the molecular weights under various conditions was measured.

Effect of He-Ne Laser on the Expressions of EGF and EGFR in Gastric Mucosa of Chronic Atrophic Gastritis

To investigate the histological change and the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in atrophic gastric mucosa in rats so as to appraise the effect of these regulators on the formation of atrophic gastritis, to study the expressions of EGF and EGFR in rats with chronic atrophic gastritis (CAG) after the irradiation of He-Ne laser, and to investigate the relations of He-Ne laser and precancerous lesion or apoptosis. The rats were divided into normal group, model group and laser group. The models of CAG rats were established with enema which was the mixed liquor consisted of sodium salicylate and alcohol, combined with irregular fasting and compulsive sporting as pathogenic factors. He-Ne laser(3.36 J/cm2 ) was used to irradiate CAG rats, once a day for 7 min, 20 days as a course of treatment; the expressions of EGF and EGFR were detected with immunohistochemical method. During the process of antral atrophy, the expressions of EGF and EGFR increased, they were higher in model group than those in normal group (P<0.05). After the irradiation of He-Ne laser, the expressions of EGF and EGFR were obviously lower than those in model group (P<0.05). We draw the conclusion as follows: gastric mucosa of model rat is in a hyper-proliferation status, with high protein expressions of apoptosis suppressor EGF and EGFR. CAG has some correlation with the imbalance between cell proliferation and apoptosis. He-Ne laser (3.36 J/cm2 ) can reduce the expressions of EGF and EGFR, which is an effective physiostimulator to stimulate the gastric mucosa of rat. The appropriate secretions of EGF and EGFR are propitious to the repair and regeneration for the gastric mucosa tissues, thus preventing CAG to canceration.

COMPARATIVE OBSERVATION ON THE CURATIVE EFFECTS OF HERPES ZOSTER TREATED BY THE TYPE JI HE-NE LASER AND POLYINOSINIC ACID

Sixty-five cases of herpes zoster were randomly divided into the He-Ne laser group(33 cases) and polyinosinic acid group (32 cases). Type JI He-Ne laser universal treatment apparatuswas used to irradiate injured cutaneous regions and to radiate Hegu (LI 4) and Zusanli (ST 36) pointswith photoconductive fibers once every day in the He-Ne laser group; 2 ml of polyinosinic acid was in-tramuscularly injected once every other day in the other group. Of the 65 cases 63 were cured, andthe two cases with sequela of neuralgia were all in the latter group. Pain was disappeared and scabswere formed respectively after 1. 48 and 5. 76 days of treatment with He-Ne laser therapy, and after10. 5 and 10. 4 days with polyinosinic acid treatment. The differences of therapeutic effects betweenthe two groups were noticeably significant (P【0. 01 ).

THE APPLICATION OF HE-NE LASER NEEDLE TO REHABILITATION OF BRAIN INJURY

20 cases of brain injury were treated for rehabilitation by He-Ne laser irradia-tion and observed on nailfold microcirculation. The optical fiber was pointed directly to the respec-tive acupoint. The selected acupoints were: Baihui (DU20), Fengfu (DU16), Fengchi (GB20),Taiyang (EX-HN5) and Hegu (LI4); each one received 4 minutes and totally 20 minutes foronce. Treatment was continued for 10 days. The immediate analgesic effect of laser reached 82.2%. The velocity in blood flow of 84.4%patients increased (P【0.01). In consequence, analgesic effect correlated closely with the velocityin the blood flow. It showed that the effect of laser therapy was superior to that of palliative treat-ment.

EFFECT OF He-Ne LASER ON THE ASTHMATIC SUBJECTS

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Treatment of folliculitis keloidalis nuchae with He-Ne Laser

AIM:To verify curative effect of He Ne laser on folliculitis keloidalis nuchae.METHODS:103 cases with folliculitis keloidalis nuchae were divided into the treatment group(73 cases) and the control group(30 cases). Local application and oral antiauxins was used in the two groups,He Ne laser radiation was used in the treatment group and then curative effect 15 days later were compared.RESULTS:The cure rats in the treatment group and control group were respectively 71.2%(52/73) and 30%(9/30)(P< 0.01) while curative days were respectively 9.2 days and 11.9 days (P< 0.01).CONCLUSION:In treatment of folliculitis Keloidalis nuchae,the application of He Ne Laser radiation can effectively reduce the course,pain and side effect of treatment,and raise the curative effect.

基于声光调制器的光学锁相环He-Ne激光稳频方法研究

报道了一种基于声光调制器与光学锁相环相结合的高稳定度激光稳频方法,用于提高热稳频He-Ne激光器的频率稳定度和准确度。为了克服全内腔热稳频He-Ne激光精密调谐困难的缺点,发展了基于声光调制器两次移频的频率调谐光路,有效地消除声光调制器移频光束路径对衍射角θ的依赖。自行研制了具有高灵敏度与捕获带宽的光学锁相环系统,利用声光调制器的高频率响应特性实现热稳频He-Ne激光高速、准确的锁定。成功实现了热稳频He-Ne激光器偏频锁定至碘稳频HeNe激光器。实验结果表明,环路锁定后拍频频率波动在±0.2 Hz范围内,频率抖动的标准差为0.04。偏置频率为30 MHz时,系统在1 s和1000 s积分时间的相对阿伦方差分别为3.3×10^(-9)和1.4×10^(-12)。系统锁定后,压缩后的拍频线宽小于2 kHz。该研究表明,采用基于声光调制器与光学锁相环相结合的激光稳频方法可以实现亚赫兹级的激光频差控制,通过将热稳频He-Ne激光器偏频锁定至高稳定度的参考激光源可以显著提升其频率稳定度和准确度。

Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA

Using agarose gel electrophoresis and T4-endo-deoxyribonuclease-V, which is a kind of restriction endonu-clease of cyclobutyl pyrimidine dimer (CPD), the impacts of He-Ne laser (5mW·mm-2) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08kJ·m-2·d-1) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested by T4-endodeoxyribonuclease-Y had been stimulated in the wheat cells.

Effects of Chinese Herbal Medicine Combined with He-Ne Laser on Lipoperoxide and Superoxide Dismutase in Chloasma Patients

Objective: To observe the effects of Chinese decoction and ligustrazin hydrochloride injection combined with He-Ne laser on lipoperoxide (LPO) and Superoxide dismutase (SOD) in patients with chloasma. Methods: 90 cases of chloasma were randomly divided into the following two groups: a treatment group (of 54 cases) treated by a self-prepared prescription for toning the kidneys and relieving the depressed liver to remove blood stasis, ligustrazin hydrochloricde injection and He-Ne laser therapy, and a control group (of 36 cases) treated with oral administration of Vitamin E and Vitamin C plus external application of 20% Azelaic acid cream. Results: The total effective rate in the treatment group was 79.6%, which was significantly higher than that of the control group (P<0.05). After treatment, the LPO level in the treatment group was significantly lowered (P<0.01), and the SOD level was significantly elevated (P<0.05). Conclusion: The therapeutic methods adopted in the treatment group may show the action of antioxidation, providing good clinical effects for treating chloasma.

Extra-short He-Ne lasers based on mode split

An extra-short He-Ne laser (shorter than 100mm) does not work well because there is likely no longitudinal mode in the lasing bandwidth sometimes and the output power rises and falls deeply from time to time.A method to solve this problem has been presented and investigated.A piece of quartz crystal plate appropriately designed is used as a cavity mirror of a He-Ne laser,which has a longitudinal mode spacing larger than the lasing bandwidth to make the equal-spacing mode split.In other words,the number of longitudinal modes is doubled and the actual mode spacing is reduced to the half of that without mode split.Therefore,there is always at least one mode operating in the lasing bandwidth and the power is stable at any room temperature.Such a laser with 85mm cavity length and 0.24mW output power has been made.