Effect of Intrauterine Laser Therapy at the IVF Success Rate on Infertile Patients with a History of IVF Failure: A Pilot Non-Blinded Study

Backgrounds: While there’s developing proof aimed toward improving embryo implantation thru a focal point on great development, restrained studies have been performed on enhancing endometrial receptivity. Intrauterine Laser Therapy (LT) can be powerful in selling endometrial cell proliferation, therefore enhancing the achievement of assisted reproductive techniques (ART). The contemporary look at aimed to research the effectiveness of effective intrauterine lasers in growing endometrial thickness and the achievement of being pregnant rate. Materials and Methods: In the current clinical randomized trial (RTC) study, the infertile women (20 - 42 years old) referred to the infertility clinic in 2023-2024 who were candidates for IVF treatment with recurrent implantation failure (RIF) history were included. The patients were divided into two main groups: the intervention group;low level laser therapy (LLLT) after hormone administration) (n = 52) and the control group (hormone administration without LT) (n = 52). The IVF success rate and change in endometrial thickness before and after the LT were compared in groups. Results: There was a significant difference between groups (p Conclusion: It appears that the incorporation of Intrauterine LT in the realm of infertility could significantly impact as a novel supplementary treatment in improving endometrial receptivity and pregnancy rate.

Effects of He-Ne laser irradiation on chronic atrophic gastritis in rats

AIM: To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats.METHODS: Sixty-three male adult Wistar rats were randomly divided into five groups including normal control group, model control group and three different dosages He-Ne laser groups. The chronic atrophic gastritis (CAG)model in rats was made by pouring medicine which was a kind of mixed liquor including 2% sodium salicylate and 30% alcohol down the throat for 8 wk to stimulate rat gastric mucosa, combining with irregular fasting and compulsive sporting as pathogenic factors; 3.36, 4.80, and 6.24J/cm2doses of He-Ne laser were used, respectively for three different treatment groups, once a day for 20 d. The pH value of diluted gastric acid was determined by acidimeter,the histopathological changes such as the inflammatory degrees in gastric mucosa, the morphology and structure of parietal cells were observed, and the thickness of mucosa was measured by micrometer under optical microscope.RESULTS: In model control group, the secretion of gastric acid was little, pathologic morphological changes in gastric mucosa such as thinner mucous, atrophic glands, notable inflammatory infiltration were found. After 3.36 J/cm2 dose of He-Ne laser treatment for 20 d, the secretion of gastric acid was increased (P＜0.05), the thickness of gastric mucosa was significantly thicker than that in model control group (P＜0.01), the gastric mucosal inflammation cells were decreased (P＜0.05). Morphology, structure and volume of the parietal cells all recuperated or were closed to normal.CONCLUSION: 3.36J/cm2 dose of He-Ne laser has a significant effect on CAG in rats.

The influence of He-Ne laser on scar formation after trabeculectomy in rabbits

AIMTo investigate the influence of He-Ne lasers on scar formation in the filtration canal after trabeculectomy in a rabbit model, as well as to explore the mechanisms for preventing scar formation when using He-Ne lasers in vivo.

The Effects of He-Ne Laser and Enhanced Ultraviolet-B Radiation on Proliferating-Cell Nuclear Antigen in Wheat Seedlings

The level of ultraviolet-B (UV-B) radiation on the Earth’s surface has increased due to depletion of the ozone layer. Here, we explored the effects of continuous wave He-Ne laser irradiation (632 nm, 5 mW·mm-2, 2 min·d-1) on proliferating-cell nuclear antigen (PCNA) damage repair function of wheat seedlings exposed to enhanced UV-B radiation (10.08 kJ&middotm-2·d-1) at the early growth stages. Wheat seedlings were irradiated with enhanced UV-B, He-Ne laser treatment or a combination of the two. We explored the transcripts of PCNA in each treatment group using RT-PCR. In addition, total proteins were extracted from the 7-day-old wheat leaves, analyzed by SDS-PAGE and identified by western blot. The results showed that the transcription of PCNA was weakened following UV-B radiation compared to the control. However, when seedlings were subjected to elevated UV-B-damaging radiation followed by He-Ne laser irradiation, the expression of PCNA was signifi-cantly higher than UV-B radiation alone. These results suggest that He-Ne laser has an active role in repairing the UV-B damaging effects. In order to further investigate the function of PCNA, dynamic arrangements of PCNA in wheat root-tip cells were observed with confocal laser scanning microscopy (CLSM). The PCNA was marked fluorescent dimming and strength weakened in en-hanced UV-B radiation (UV-B) compared with the control group (CK) during processing. It shows that PCNA may be involved in the separation of chromosomes.

Protecting effect of He-Ne laser on winter wheat from UV-B radiation damage by analyzing proteomic changes in leaves

The ultraviolet-B (UV-B) radiation can affect gene expression of plant in growth and development. Winter wheat (Triticum aestivum), as a kind of economic crop cultured in the Northern China, is also damaged by present-day ultraviolet-B (UV-B) radiation. It was reported that He-Ne lasers could alleviate cell damage caused by UV-B radiation in plants. To get the proteomic changes of wheat alleviated by He-Ne lasers, we studied protein expression profiles of winter wheat (Jin Mai NO.79) leaves by running 2-DE (two-dimensional gel electrophoresis), which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, in which they were confirmed to be winter wheat proteins. The results showed: 1) the proteomic changes between the ultraviolet-B radiation group and normal light group were significantly different on sixth day;2) The expression of some proteins such as catalase, DNA polymerase and reduced glutathione declines at the ultraviolet-B radiation group and increases at the UV-B + He-Ne laser group. This indicates UV-B could regulate genes encoding proteins in Winter wheat leaves and affect the growth of the Winter wheat. He-Ne lasers treatment could significantly alleviate UV-B-induced damage in wheat.

Enhancement of Human Blood Storage Period by Irradiation of Low Level He-Ne Laser

The aim of the present work is to investigate the effect of the He-Ne laser irradiation on the whole human blood (HB) in order to enhance the conditions of conservation. The HB was irradiated by He-Ne laser;(λ = 632 nm, continuous wave, power 30 mW, 2 mm diameter beam spot), electrical properties and complete blood count CBC were measured at three doses (0.0287, 0.0563 and 0.198 J/cm3) to the relevant best exposure dose during storage periods 9, 24, 30, 35 & 50 days. The irradiation process with the selected dose was performed by the exposure of the laser beam on the blood sample flow through narrow tube of cross section area, 0.0831 cm2. Blood dielectric parameters, (electric conductivity, dielectric constant, dielectric loss and dipole moment) and CBC, (red blood cell, white blood cell, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, and mean cell or corpuscular hemoglobin in concentration) were measured. The obtained results were compared with that of the control and showed that the best irradiation exposure dose suitable for increasing the time of blood storage with minimum changes in properties is 0.198 J/cm3 and storage period of about 50 days. The present study revealed that irradiation by He-Ne laser could be considered a good means to improve the conservation conditions of human blood.

Femtosecond laser ultrafast photothermal exsolution

Exsolution,as an effective approach to constructing particle-decorated interfaces,is still challenging to yield interfacial films rather than isolated particles.Inspired by in vivo near-infrared laser photothermal therapy,using 3 mol%Y_(2)O_(3)stabilized tetragonal zirconia polycrystals(3Y-TZP)as host oxide matrix and iron-oxide(Fe3O4/γ-Fe_(2)O_(3)/α-Fe_(2)O_(3))materials as photothermal modulator and exsolution resource,femtosecond laser ultrafast exsolution approach is presented enabling to conquer this challenge.The key is to trigger photothermal annealing behavior via femtosecond laser ablation to initialize phase transition from monoclinic zirconia(m-ZrO_(2))to tetragonal zirconia(t-ZrO_(2))and induce t-ZrO_(2)columnar crystal growth.Fe-ions rapidly segregate along grain boundaries and diffuse towards the outmost surface,and become‘frozen’,highlighting the potential to use photothermal materials and ultrafast heating/quenching behaviors of femtosecond laser ablation for interfacial exsolution.Triggering interfacial iron-oxide coloring exsolution is composition and concentration dependent.Photothermal materials themselves and corresponding photothermal transition capacity play a crucial role,initializing at 2 wt%,3 wt%,and 5 wt%for Fe3O4/γ-Fe_(2)O_(3)/α-Fe_(2)O_(3)doped 3Y-TZP samples.Due to different photothermal effects,exsolution states of ablated 5 wt%Fe_(3)O_(4)/γ-Fe_(2)O_(3)/α-Fe_(2)O_(3)-doped 3Y-TZP samples are totally different,with whole coverage,exhaustion(ablated away)and partial exsolution(rich in the grain boundaries in subsurface),respectively.Femtosecond laser ultrafast photothermal exsolution is uniquely featured by up to now the deepest microscale(10μm from 5 wt%-Fe_(3)O_(4)-3Y-TZP sample)Fe-elemental deficient layer for exsolution and the whole coverage of exsolved materials rather than the formation of isolated exsolved particles by other methods.It is believed that this novel exsolution method may pave a good way to modulate interfacial properties for extensive applications in the fields of biology,optics/photonics,energy,catalysis,environment,etc.

The Photopolymerization Kinetics of Acryl amide Sensitized with Toluidine Blue-Alcoholamine Induced by He-Ne Laser

Toluidine blue(TB) is a good sensitizer for the polymerization of acrylnmide(AM) induced by He-Ne laser. With TB as a sensitizer and various alcoholamine as donors, the photoredox of TB and the polymerization kinetics of AM induced by He-Ne laser were investigated. It was found that either for the photoredox of TB or for the polymerization of AM, the activity of alcoholamine increases in the order of EOA<DEOA <TEOA in TB-alcoholamine sensitized system. The overall apparent kinetic equations of AM polymerization at 30℃ have been determined. In addition, the molecular weights under various conditions was measured.

THE PHOTOPOLYMERIZATION KINETICS OF PHOTOSENSITIZED ACRYLAMIDE SYSTEM INDUCED BY He-Ne LASER

The kinetics of the photopolymerization of sensitized acrylamide (AM) system induced by He-Ne laser has been investigated. Using methylene blue (MB)-triethanolamine (TEOA) as the photosensitive system, the photopolymerization followed a nonsteady-state kinetic scheme in the initial period of polymerization (the monomer conversion C%<2%) and then followed a steady-state kinetic scheme (5% >C%>2%). Accirding to the steady-state hypothesis, the mechanism of photopolymerization was proposed. The deduced kinetic equation of the photopolymerization of AM is in good coincidence with the experimental results.

Anomalous Variation of Beat Frequency in a Dual Frequency He-Ne Laser

The beat frequency in a dual frequency He-Ne laser varies while the resonant cavity length is tuned. As to the laser with two longitudinal modes, the variation amplitude is commonly less than 500 kHz, proven by experiments and theories. This study reveals an anomalous variation of the beat frequency when a piece of element is put into the cavity and is aligned with the laser axis. Consequently the variation amplitude couM reach 22 MHz, several dozen times larger than that without the intra-cavity element. This cannot be explained only by laser mode pulling and pushing effects. Some influencing factors are investigated experimentally, including the tilted angle of the element and the distance between its surface and cavity mirror. The qualitative analysis is discussed, which agrees with the experimental results.

The Influence of Diode and He-Ne Lasers on Corn and Wheat Seeds

The paper presents a study on the effects of low intensity laser irradiation on morphological changes in plants sprouted from maize hybrid seeds （two hybrids） and wheat seeds. Pre-sowing laser irradiation treatment on the seeds was done, intervals from 10 s to 15 min （approximately）, by using a diode laser output power of 12 mW at 904 nm wavelength or with He-Ne laser with output power of 50 mW and 632.8 nm wavelength. Before irradiation seeds were divided into groups （wet and dry, and then in subgroups-irradiated or control groups）. We used maize hybrids, Amilacea and Identata and wheat （Triticum aestivum）. The reflection coefficient in visible range was done for maize varieties. Obtained data show the influence of laser beam to better plant growth. Better results are obtained for dry seed irradiation than for wet. In order to investigate the effect of laser beam and in general to clarify a lot of unsolved photo processes related to bioorganisms at macroscopic and microscopic levels, some optical constants of selected plant families were researched. At the same time, the influence of laser beams of common wavelengths to the selected plants was monitored. Morphological processes of plants （seeds and leaves） irradiated under different conditions and plant growing dynamics were contemplated. The definite correlation analyses of obtained results were made, clearly speaking about the influence of small-dose radiation to characteristics （quantitative and other genetic, bio-stimulating effects） of future plant growth.

Efficacy of He-Ne Laser in Combination with Topical Clindamycin in the Treatment of Acne Vulgaris (AV)

Objective: To evaluate the efficacy of He-Ne laser in combination with topical clindamycin in the treatment of AV at 108 Military Central Hospital from Oct 2015 to Aug 2016. Subjects and Methods: 61 patients with AV were divided into 2 groups: Group 1: 31 patients were treated with He-Ne laser in combination with topical Clindamycin, Group 2: 30 patients were only treated with topical clindamycin. Laser HE-NE was applied 2 times/week for 6 weeks. Result: After 3 months of treatment Group 1: very good—51.6%, good—48.4%. Group 2: very good—30%, good—50% and moderate—20%. Conclusion: The combination of He-Ne laser with topical clindamycin shows more rapid clinical improvement compared to topical clindamycin alone in the treatment of mild and moderate AV.

Effect of He-Ne Laser on the Expressions of EGF and EGFR in Gastric Mucosa of Chronic Atrophic Gastritis

To investigate the histological change and the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in atrophic gastric mucosa in rats so as to appraise the effect of these regulators on the formation of atrophic gastritis, to study the expressions of EGF and EGFR in rats with chronic atrophic gastritis (CAG) after the irradiation of He-Ne laser, and to investigate the relations of He-Ne laser and precancerous lesion or apoptosis. The rats were divided into normal group, model group and laser group. The models of CAG rats were established with enema which was the mixed liquor consisted of sodium salicylate and alcohol, combined with irregular fasting and compulsive sporting as pathogenic factors. He-Ne laser(3.36 J/cm2 ) was used to irradiate CAG rats, once a day for 7 min, 20 days as a course of treatment; the expressions of EGF and EGFR were detected with immunohistochemical method. During the process of antral atrophy, the expressions of EGF and EGFR increased, they were higher in model group than those in normal group (P<0.05). After the irradiation of He-Ne laser, the expressions of EGF and EGFR were obviously lower than those in model group (P<0.05). We draw the conclusion as follows: gastric mucosa of model rat is in a hyper-proliferation status, with high protein expressions of apoptosis suppressor EGF and EGFR. CAG has some correlation with the imbalance between cell proliferation and apoptosis. He-Ne laser (3.36 J/cm2 ) can reduce the expressions of EGF and EGFR, which is an effective physiostimulator to stimulate the gastric mucosa of rat. The appropriate secretions of EGF and EGFR are propitious to the repair and regeneration for the gastric mucosa tissues, thus preventing CAG to canceration.

He-Ne激光穴位照射对小鼠腹腔巨噬细胞功能的影响

目的 探讨He Ne激光对机体的免疫刺激作用。方法 用能量密度为 6 3 7J/cm2 的He Ne激光照射小鼠的神阙穴 ,时间分别为 3、5、7和 10天 ,每天照射 1次 ,每次 5min。激光照射结束后取小鼠腹腔巨噬细胞 ,分别做如下实验 :(1)用荧光显微镜观察腹腔巨噬细胞对白色念珠菌的吞噬功能 ;(2 )用α 荼酚醋酸酯法显示腹腔巨噬细胞内非特异性酯酶 (NSE) ,并用图像分析系统对NSE作定量分析 ;(3)用透射电镜观察腹腔巨噬细胞超微结构的变化。结果 各激光穴位照射组小鼠腹腔巨噬细胞对白色念珠菌的吞噬率、吞噬指数及NSE的各项参数值与对照组比较 ,差异均有非常显著意义 (P <0 0 1)。照射天数增加 ,各项指标也随之增加 ,各照射组间比较 ,差异也均有非常显著意义 (P <0 0 1)。另外 ,照射 10天组腹腔巨噬细胞的超微结构呈活化状态。结论 用适当剂量的He Ne激光照射小鼠神阙穴能活化其巨噬细胞 ,从而提高机体免疫功能。

低能量He-Ne激光血管内照射疗法在脑囊虫病后遗症治疗中的应用

目的 探讨脑囊虫病所致后遗症的疗法。 方法 采用低能量 He- Ne激光血管内照射疗法 (IL IB)对脑囊虫病后遗症者进行治疗。 结果 多数病人症状得到明显改善 ,其中头痛、头晕临床治愈率分别为 17.2 4%和 6 4.71% ;头痛、头晕和记忆力减退总有效率达 10 0 % ;在癫痫发作控制方面 ,总有效率 (包括显效和有效 )达 76 .19% ;IL IB治疗后81.82 %的病人脑电图及脑电地形图出现明显改善。 结论 IL

弱He-Ne激光血管内照射治疗急性缺血性脑血管病疗效及机理探讨

本研究观察了１２０例颈内动脉系统急性缺血性脑血管疾病患者，按单双数随机分入激光综合治疗组（激光组）和常规药物治疗组（药物组）各６０例。均予静脉点滴通脉液５００ｍｌ，川芎嗪１００ｍｇ，每天一次，十天为一疗程，激光组同时予１ＬＬＬ１治疗，照射时间６０分钟，每天一次，十天为一疗程，光纤末端输出功率１．５－２．０ｍＷ，同时予３０例健康志愿者１ＬＬＬ１一次。所有受试者在治疗前后均进行血液流变学指标，纤溶系统指标和ＴＣＤ检测，并评定患者治疗５、１０天的疗效。研究结果证明，１ＬＬＬ１ＩＴ可以提高单一药物治疗的疗效，机制是改善血液流变学性能，改善微循环，增快脑血流速度，而且可能有激活纤溶系统功能的作用。所有接受１ＬＬＬＩＴ者均无明显的不良反应。本临床研究表明，１ＬＬＬＩＴ可作为缺血性脑血管病一项有效的安全的辅助治疗方法。

He-Ne激光照射小鼠睾丸的光镜和电镜观察

以二种剂量的Ｈｅ－Ｎｅ激光分别照射二组雄性小鼠的阴囊，取睾丸组织作光镜和电镜观察。实验所用激光剂量分别为临床常用最大剂量的２０倍和７５倍。实验结果表明，实验动物生精上皮无紊乱和松散现象，各级生精细胞、成熟精子、支持细胞及睾丸间质细胞的超微结构无明显损伤。表明所用的Ｈｅ－Ｎｅ激光无抗生精作用，不会影响生殖细胞的增殖及雄性激素的分泌，提示临床治疗所用Ｈｅ－Ｎｅ激光的剂量安全可靠，不致引起病人生殖细胞损伤及性功能的改变。

光动力学疗法与He-Ne激光照射脾区联合治疗荷瘤小鼠的实验研究

由于激光的穿透深度有限 ,光动力学疗法对深部及较大体积的实体瘤治疗效果不佳 ,目前人们致力于PDT治疗与传统治疗方法联合应用的研究。本实验以小鼠S1 80 肉瘤为研究对象 ,观察PDT与He -Ne激光照射脾区联合治疗对小鼠肿瘤的抑制率和生存期的影响 ,结果 :联合治疗组对肿瘤的抑制率最高 ,生存期最长。

低强度He-Ne激光对血液粘度的影响

本文简要叙述了激光生物效应和弱激光生物刺激作用,阐述了激光照射血液所产生的生物学效应。在弱激光血管内照射疗法(ILLLIT)治疗后血液流变学指标均有显著改善的临床结果基础上,对该治疗方法的治疗机理进行了理论研究,叙述了弱激光对红细胞聚集指数和红细胞变形性的改善作用,并探讨其可能的作用机理。

He-Ne激光穴位照射治疗胆石症310例

为了探索胆石症非手术治疗的新方法，以ＨｅＮｅ激光穴位照射治疗胆石症３１０例。治愈率３５２％，好转率５４５％，总有效率８９７％，无效率１０３％；总排石率６００％，疗效优于电针对照组（Ｐ＜００１）。疗效分析表明，胆总管结石及直径≤１０ｃｍ结石的疗效较好。对３０名健康人进行ＨｅＮｅ激光穴位照射对胆囊、胆总管效应变化的Ｂ型超声观察。结果提示，激光照射期门、日月和胆俞穴可使胆囊收缩，胆总管扩张，为治疗机制提供了理论依据。