Objective:To investigate capacity of Hyptis suaveolens(H.suaveolens) methanol extract as an antioxidant to protect against carbon tetrachloride(CCl_4)-indueed oxidative stress,hepatotoxicitv in Albino Wistar rats and ...Objective:To investigate capacity of Hyptis suaveolens(H.suaveolens) methanol extract as an antioxidant to protect against carbon tetrachloride(CCl_4)-indueed oxidative stress,hepatotoxicitv in Albino Wistar rats and cytoprotective effect of hydrogen peroxide(H_2O_2) induced cell death in HepG_2 cell line.Methods:Two different doses of methanol extract of H.suaveolens were evaluated for the hepatoprotective activity against carbon tetrachloride(CCl_4) induced hepatotoxicitv in rats.Animals in GroupⅠ:served as control,groupⅡ:H.suaveolens(100 mL/ kg b.w),groupⅢ:H.suaveolens(50 mL/kg b.w) + CCl_4(1 mg/kg),groupⅣ:H.suaveolens(100 mL/kg b.w) + CCl_4(1 ml/kg)and groupⅤ:CCl_4(1 mL/kg).Histopathologic changes of liver were also evaluated.Cytotoxicity was also determined by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide(MTT) assay.Results:Oral sigle dose treatment of CCl_4 produced a marked elevation in the serum levels of aspartate transaminase(AST),alanine transaminase(ALT), alkaline phosphatase(ALP) and Lactate dehydrogenase(LDH).Histopathological analysis of the liver of CCl_4-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD,GSH,GST,GR and LPO.Treatment with H_2O_2 significantly induced death of HepG_2 cell.Pretreatment with H.suaveolens methanol extract inhibited or attenuated H_2O_2 induced cytotoxicity.Conclusions:This study shows that H.suaveolens methanol extract can be proposed to protect the liver against CCl_4-induced oxidative damage in rats and protect the cells against H_2O_2-induced oxidative damage in HepG_2 cells.The hepatoprotective and cytoprotective effects might be correlated with its antioxidant and free radical scavenger effects.展开更多
基金supported by Ministry of Human Resource Developmenl and University Grant Commission Under Institution of Excellence Scheme awarded to the University of Mysore
文摘Objective:To investigate capacity of Hyptis suaveolens(H.suaveolens) methanol extract as an antioxidant to protect against carbon tetrachloride(CCl_4)-indueed oxidative stress,hepatotoxicitv in Albino Wistar rats and cytoprotective effect of hydrogen peroxide(H_2O_2) induced cell death in HepG_2 cell line.Methods:Two different doses of methanol extract of H.suaveolens were evaluated for the hepatoprotective activity against carbon tetrachloride(CCl_4) induced hepatotoxicitv in rats.Animals in GroupⅠ:served as control,groupⅡ:H.suaveolens(100 mL/ kg b.w),groupⅢ:H.suaveolens(50 mL/kg b.w) + CCl_4(1 mg/kg),groupⅣ:H.suaveolens(100 mL/kg b.w) + CCl_4(1 ml/kg)and groupⅤ:CCl_4(1 mL/kg).Histopathologic changes of liver were also evaluated.Cytotoxicity was also determined by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide(MTT) assay.Results:Oral sigle dose treatment of CCl_4 produced a marked elevation in the serum levels of aspartate transaminase(AST),alanine transaminase(ALT), alkaline phosphatase(ALP) and Lactate dehydrogenase(LDH).Histopathological analysis of the liver of CCl_4-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD,GSH,GST,GR and LPO.Treatment with H_2O_2 significantly induced death of HepG_2 cell.Pretreatment with H.suaveolens methanol extract inhibited or attenuated H_2O_2 induced cytotoxicity.Conclusions:This study shows that H.suaveolens methanol extract can be proposed to protect the liver against CCl_4-induced oxidative damage in rats and protect the cells against H_2O_2-induced oxidative damage in HepG_2 cells.The hepatoprotective and cytoprotective effects might be correlated with its antioxidant and free radical scavenger effects.
