INTRODUCTIONEsophageal variceal bleeding (EVB) is one of themost common complications of cirrhosis with portalhypertension.In recent years,great progress hasbeen made in medicinal treatment.Somatostatin hasbeen widely...INTRODUCTIONEsophageal variceal bleeding (EVB) is one of themost common complications of cirrhosis with portalhypertension.In recent years,great progress hasbeen made in medicinal treatment.Somatostatin hasbeen widely used in clinics,for it can effectivelylower the portal venous pressure (PVP) with展开更多
This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension.Methods Expression profiles GSE2378 and GSE758 including 27 react...This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension.Methods Expression profiles GSE2378 and GSE758 including 27 reactive optic nerve head astrocytes(ONHAs)by hypertensions and 26 normal controls,were integrated and deeply analyzed.Differentially expressed genes(DEGs)were sorted and candidate genes and pathways enrichment were analyzed.DEGs-associated protein-protein interaction network(PPI)was performed.Results A total of 119 consistently expressed genes were identified from 281 commonly changed DEGs,including 68 up-regulated genes and 51 down-regulated genes.PPI network complex filtered 75 DEGs(43 up-regulated and 32 down-regulated genes)of the 119 consistently altered DEGs and developed 117 edges,and 10 hub genes were identified.The most significant 3 modules were filtered from PPI,pathway enrichment analysis showed that module 1 was associated with extracellular exosome.Module 2 was mainly associated with antibody-dependent cellular cytotoxicity(ADCC)and module 3 was mainly associated with Hippo signaling pathway.Conclusion Taken above,using integrated bioinformatical analysis,we have identified DEGs candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension,which could improve our understanding of the cause and underlying molecular events,and these candidate genes and pathways could be therapeutic targets for glaucoma.展开更多
Hydrogen sulfide (H 2S) is recently found to be a new gaseous messenger playing an important role in many physiological and pathophysiological processes. The aim of this study was to explore the changes in endogenous ...Hydrogen sulfide (H 2S) is recently found to be a new gaseous messenger playing an important role in many physiological and pathophysiological processes. The aim of this study was to explore the changes in endogenous H 2S pathway and examine the effects of H 2S on the development of spontaneous hypertensive rats (SHR). A controlled study on SHR and WKY rats at the age of 4 weeks showed that after 5 weeks of experiment, the blood pressure of SHR was markedly increased as compared with WKY rats (183.57±11.80 mm Hg vs 107.5± 22.68 mm Hg, P <0.05)and the ratio of left heart weight to whole heart weight of SHR was also increased as compared with WKY rats (0.85±0.02 vs 0.83±0.02, P <0.05). While, the aortic H 2S producing rate and H 2S plasma level were decreased in SHR compared with WKY rats (15.63±2.89 nmol·min -1 ·g -1 vs 25.31± 5.99 nmol·min -1 ·g -1 , 20.35±9.20 μmol·L -1 vs 48.40±13.36 μmol·L -1 , P <0.05). NaHS, however, attenuated the high blood pressure and the ratio of left heart weight to whole heart weight of SHR (158.13± 12.52 mm Hg vs 183.57±11.80 mm Hg and 0.83±0.03 vs 0.85±0.02), respectively. The above findings suggested that the reduced production of endogenous H 2S was important in the development of spontaneous hypertension. The authors also observed that 5 weeks after the experiment the relaxing rate of aortic rings in response to acetylcholine in SHR was higher than that of WKY rats and NaHS enhanced the relaxingresponse of aortic rings to acetylcholine in SHR significantly( P <0.05). The aortic relaxing activities in both SHR and WKY rats displayed a dose dependent response to different doses of NaHS. In conclusion, the reduced production of endogenous H 2S in aorta is involved in the pathogenesis of spontaneous hypertension and is of great biological importance in modulating vasorelaxation.展开更多
基金the National Natural Science Foundation and Ministry of Public Health of China,No.39500141
文摘INTRODUCTIONEsophageal variceal bleeding (EVB) is one of themost common complications of cirrhosis with portalhypertension.In recent years,great progress hasbeen made in medicinal treatment.Somatostatin hasbeen widely used in clinics,for it can effectivelylower the portal venous pressure (PVP) with
基金support from the China National Natural Science Foundation Funding Project(NO.81804150)Hunan University of Chinese Medicine,National Key Discipline of TCM Diagnostics Foundation Funding Project(No.2015ZYZD02)+5 种基金The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese MedicineHunan Provincial Department of Education Innovation Platform Open Fund Project(16K065)Chinese Medicine Key Laboratory of Prevention and Treatment of Disease in Hunan Province(2017TP1018)Changsha Science and Technology Plan Project(KC1704005)Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese MedicineHunan Provincial Research Innovation Project for Graduate students(CX2017B426)
文摘This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension.Methods Expression profiles GSE2378 and GSE758 including 27 reactive optic nerve head astrocytes(ONHAs)by hypertensions and 26 normal controls,were integrated and deeply analyzed.Differentially expressed genes(DEGs)were sorted and candidate genes and pathways enrichment were analyzed.DEGs-associated protein-protein interaction network(PPI)was performed.Results A total of 119 consistently expressed genes were identified from 281 commonly changed DEGs,including 68 up-regulated genes and 51 down-regulated genes.PPI network complex filtered 75 DEGs(43 up-regulated and 32 down-regulated genes)of the 119 consistently altered DEGs and developed 117 edges,and 10 hub genes were identified.The most significant 3 modules were filtered from PPI,pathway enrichment analysis showed that module 1 was associated with extracellular exosome.Module 2 was mainly associated with antibody-dependent cellular cytotoxicity(ADCC)and module 3 was mainly associated with Hippo signaling pathway.Conclusion Taken above,using integrated bioinformatical analysis,we have identified DEGs candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension,which could improve our understanding of the cause and underlying molecular events,and these candidate genes and pathways could be therapeutic targets for glaucoma.
文摘Hydrogen sulfide (H 2S) is recently found to be a new gaseous messenger playing an important role in many physiological and pathophysiological processes. The aim of this study was to explore the changes in endogenous H 2S pathway and examine the effects of H 2S on the development of spontaneous hypertensive rats (SHR). A controlled study on SHR and WKY rats at the age of 4 weeks showed that after 5 weeks of experiment, the blood pressure of SHR was markedly increased as compared with WKY rats (183.57±11.80 mm Hg vs 107.5± 22.68 mm Hg, P <0.05)and the ratio of left heart weight to whole heart weight of SHR was also increased as compared with WKY rats (0.85±0.02 vs 0.83±0.02, P <0.05). While, the aortic H 2S producing rate and H 2S plasma level were decreased in SHR compared with WKY rats (15.63±2.89 nmol·min -1 ·g -1 vs 25.31± 5.99 nmol·min -1 ·g -1 , 20.35±9.20 μmol·L -1 vs 48.40±13.36 μmol·L -1 , P <0.05). NaHS, however, attenuated the high blood pressure and the ratio of left heart weight to whole heart weight of SHR (158.13± 12.52 mm Hg vs 183.57±11.80 mm Hg and 0.83±0.03 vs 0.85±0.02), respectively. The above findings suggested that the reduced production of endogenous H 2S was important in the development of spontaneous hypertension. The authors also observed that 5 weeks after the experiment the relaxing rate of aortic rings in response to acetylcholine in SHR was higher than that of WKY rats and NaHS enhanced the relaxingresponse of aortic rings to acetylcholine in SHR significantly( P <0.05). The aortic relaxing activities in both SHR and WKY rats displayed a dose dependent response to different doses of NaHS. In conclusion, the reduced production of endogenous H 2S in aorta is involved in the pathogenesis of spontaneous hypertension and is of great biological importance in modulating vasorelaxation.
