INTRODUCTIONEsophageal variceal bleeding (EVB) is one of themost common complications of cirrhosis with portalhypertension.In recent years,great progress hasbeen made in medicinal treatment.Somatostatin hasbeen widely...INTRODUCTIONEsophageal variceal bleeding (EVB) is one of themost common complications of cirrhosis with portalhypertension.In recent years,great progress hasbeen made in medicinal treatment.Somatostatin hasbeen widely used in clinics,for it can effectivelylower the portal venous pressure (PVP) with展开更多
This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension.Methods Expression profiles GSE2378 and GSE758 including 27 react...This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension.Methods Expression profiles GSE2378 and GSE758 including 27 reactive optic nerve head astrocytes(ONHAs)by hypertensions and 26 normal controls,were integrated and deeply analyzed.Differentially expressed genes(DEGs)were sorted and candidate genes and pathways enrichment were analyzed.DEGs-associated protein-protein interaction network(PPI)was performed.Results A total of 119 consistently expressed genes were identified from 281 commonly changed DEGs,including 68 up-regulated genes and 51 down-regulated genes.PPI network complex filtered 75 DEGs(43 up-regulated and 32 down-regulated genes)of the 119 consistently altered DEGs and developed 117 edges,and 10 hub genes were identified.The most significant 3 modules were filtered from PPI,pathway enrichment analysis showed that module 1 was associated with extracellular exosome.Module 2 was mainly associated with antibody-dependent cellular cytotoxicity(ADCC)and module 3 was mainly associated with Hippo signaling pathway.Conclusion Taken above,using integrated bioinformatical analysis,we have identified DEGs candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension,which could improve our understanding of the cause and underlying molecular events,and these candidate genes and pathways could be therapeutic targets for glaucoma.展开更多
目的：探讨单中心中国人直立性高血压（orthostatic hypertension，OHT）的年龄和性别差异。方法：选择2000年1月至2012年8月在中南大学湘雅二医院晕厥专科门诊就诊或住院的不明原因头晕、晕厥等患者2 994例进行直立倾斜试验（head-up t...目的：探讨单中心中国人直立性高血压（orthostatic hypertension，OHT）的年龄和性别差异。方法：选择2000年1月至2012年8月在中南大学湘雅二医院晕厥专科门诊就诊或住院的不明原因头晕、晕厥等患者2 994例进行直立倾斜试验（head-up tilt test，HUTT）检查，年龄2.00~78.00（19.07±14.78）岁，男1 406例，女1 588例。符合OHT者745例，分为成人组（≥18岁，247例）和儿童组（〈18岁，498例），分析OHT出现率、OHT类型及HUTT后3 min血压增加量的年龄与性别差异。结果：1）OHT出现率为24.88%（745/2 994），男女比较差异未见统计学意义（25.75% vs 24.12%，χ2=1.058，P〉0.05）；成人组较儿童组出现率高（27.05% vs 23.83%，χ2=4.125，P〈0.05）；745例OHT患者中52例（6.98%）表现为直立后收缩压、舒张压均升高（sOHT合并dOHT），16例（2.15%）为单纯直立性高收缩压（sOHT），677例（90.87%）为单纯直立性高舒张压（dOHT）。成人组单纯sOHT出现率、sOHT合并dOHT出现率均明显高于儿童组（分别为1.11% vs 0.29%，χ2=7.965，P〈0.01；2.88% vs 1.24%，χ2=9.849，P〈0.01）；成人组单纯dOHT出现率与儿童组比较差异未见统计学意义（23.56% vs 22.20%，χ2=0.668，P〉0.05）；sOHT合并dOHT与单纯dOHT在不同性别组中出现率差异未见统计学意义（男：1.71% vs 1.76%，χ2=0.014，P〉0.05；女：23.68% vs 21.66%，χ2=1.742，P〉0.05）；但儿童中dOHT出现率男性高于女性（24.53% vs 19.74%，χ2=6.933，P〈0.05）。2）与儿童组比较，成人组sOHT合并dOHT收缩压增加量[（25.62±4.96） mmHg vs （23.54±5.83） mmHg，t=1.385，P〉0.05]、dOHT舒张压增加量[（13.46±3.49） mmHg vs （13.23±3.22） mmHg， t=0.840，P〉0.05]差异均无统计学意义。与儿童组比较，成人组sOHT收缩压增加量[（25.44±4.96） mmHg vs （23.68±5.35 ） mmHg，t=1.411，P〉0.05]及dOHT舒张压增加量[（14.09±4.28） mmHg vs （13.05±3.82） mmHg，t=1.887，P〉0.05]差异均未见统计学意义。结论：OHT出现率成人高于儿童，以dOHT多见，儿童以男性多见。在sOHT合并dOHT与单纯dOHT中，直立后3 min血压增加量成人与儿童未见差异。展开更多
基金the National Natural Science Foundation and Ministry of Public Health of China,No.39500141
文摘INTRODUCTIONEsophageal variceal bleeding (EVB) is one of themost common complications of cirrhosis with portalhypertension.In recent years,great progress hasbeen made in medicinal treatment.Somatostatin hasbeen widely used in clinics,for it can effectivelylower the portal venous pressure (PVP) with
基金support from the China National Natural Science Foundation Funding Project(NO.81804150)Hunan University of Chinese Medicine,National Key Discipline of TCM Diagnostics Foundation Funding Project(No.2015ZYZD02)+5 种基金The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese MedicineHunan Provincial Department of Education Innovation Platform Open Fund Project(16K065)Chinese Medicine Key Laboratory of Prevention and Treatment of Disease in Hunan Province(2017TP1018)Changsha Science and Technology Plan Project(KC1704005)Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese MedicineHunan Provincial Research Innovation Project for Graduate students(CX2017B426)
文摘This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension.Methods Expression profiles GSE2378 and GSE758 including 27 reactive optic nerve head astrocytes(ONHAs)by hypertensions and 26 normal controls,were integrated and deeply analyzed.Differentially expressed genes(DEGs)were sorted and candidate genes and pathways enrichment were analyzed.DEGs-associated protein-protein interaction network(PPI)was performed.Results A total of 119 consistently expressed genes were identified from 281 commonly changed DEGs,including 68 up-regulated genes and 51 down-regulated genes.PPI network complex filtered 75 DEGs(43 up-regulated and 32 down-regulated genes)of the 119 consistently altered DEGs and developed 117 edges,and 10 hub genes were identified.The most significant 3 modules were filtered from PPI,pathway enrichment analysis showed that module 1 was associated with extracellular exosome.Module 2 was mainly associated with antibody-dependent cellular cytotoxicity(ADCC)and module 3 was mainly associated with Hippo signaling pathway.Conclusion Taken above,using integrated bioinformatical analysis,we have identified DEGs candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension,which could improve our understanding of the cause and underlying molecular events,and these candidate genes and pathways could be therapeutic targets for glaucoma.
文摘目的：探讨单中心中国人直立性高血压（orthostatic hypertension，OHT）的年龄和性别差异。方法：选择2000年1月至2012年8月在中南大学湘雅二医院晕厥专科门诊就诊或住院的不明原因头晕、晕厥等患者2 994例进行直立倾斜试验（head-up tilt test，HUTT）检查，年龄2.00~78.00（19.07±14.78）岁，男1 406例，女1 588例。符合OHT者745例，分为成人组（≥18岁，247例）和儿童组（〈18岁，498例），分析OHT出现率、OHT类型及HUTT后3 min血压增加量的年龄与性别差异。结果：1）OHT出现率为24.88%（745/2 994），男女比较差异未见统计学意义（25.75% vs 24.12%，χ2=1.058，P〉0.05）；成人组较儿童组出现率高（27.05% vs 23.83%，χ2=4.125，P〈0.05）；745例OHT患者中52例（6.98%）表现为直立后收缩压、舒张压均升高（sOHT合并dOHT），16例（2.15%）为单纯直立性高收缩压（sOHT），677例（90.87%）为单纯直立性高舒张压（dOHT）。成人组单纯sOHT出现率、sOHT合并dOHT出现率均明显高于儿童组（分别为1.11% vs 0.29%，χ2=7.965，P〈0.01；2.88% vs 1.24%，χ2=9.849，P〈0.01）；成人组单纯dOHT出现率与儿童组比较差异未见统计学意义（23.56% vs 22.20%，χ2=0.668，P〉0.05）；sOHT合并dOHT与单纯dOHT在不同性别组中出现率差异未见统计学意义（男：1.71% vs 1.76%，χ2=0.014，P〉0.05；女：23.68% vs 21.66%，χ2=1.742，P〉0.05）；但儿童中dOHT出现率男性高于女性（24.53% vs 19.74%，χ2=6.933，P〈0.05）。2）与儿童组比较，成人组sOHT合并dOHT收缩压增加量[（25.62±4.96） mmHg vs （23.54±5.83） mmHg，t=1.385，P〉0.05]、dOHT舒张压增加量[（13.46±3.49） mmHg vs （13.23±3.22） mmHg， t=0.840，P〉0.05]差异均无统计学意义。与儿童组比较，成人组sOHT收缩压增加量[（25.44±4.96） mmHg vs （23.68±5.35 ） mmHg，t=1.411，P〉0.05]及dOHT舒张压增加量[（14.09±4.28） mmHg vs （13.05±3.82） mmHg，t=1.887，P〉0.05]差异均未见统计学意义。结论：OHT出现率成人高于儿童，以dOHT多见，儿童以男性多见。在sOHT合并dOHT与单纯dOHT中，直立后3 min血压增加量成人与儿童未见差异。
