Effects of hepatitis B virus infection on human sperm chromosomes

AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B patients.METHODS: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.RESULTS: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was random.CONCLUSION: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/or induction chromosome aberrations, as well as the possibility of vertical transmission of HBV via the germ line to the next generation.

深圳特区717名健康成人锌原卟啉(ZPP)参考值的探讨

在排除缺铁性贫血的情况下，血液ＺＰＰ值可作为铅中毒的诊断指标之一。对７１７名未接触铅的健康成人做血液ＺＰＰ值测定，结果其９９％上限值为１．５９５μｍｏｌ／Ｌ（７．１２μｇ／ｇＨｂ）。考虑到地区的差异性和标准的统一性，我们推荐深圳市区ＺＰＰ参考值上限定为１．５７μｍｏｌ／Ｌ（７．０μｇ／ｇＨｂ）。

石家庄地区部分健康成人血清尿酸参考值调查分析

目的确定石家庄地区健康成人不同性别、不同年龄组的血清尿酸参考值范围。方法将1721例自20～60岁每间隔10岁男、女各分为一组,60岁以上者分为男、女两组,每组至少120例;将<50岁的各年龄组视为青壮年,>50岁年龄组视为中老年。均采用罗氏全自动生化分析仪检测血清尿酸浓度。结果本调查研究中男、女血清尿酸浓度均呈正态分布,经统计学分析表明,同年龄组男性和女性血清尿酸浓度比较差异有统计学意义(P<0.001);男性、女性青壮年组与中老年组血清尿酸浓度比较差异亦有统计学意义(P<0.001)。男性除20～29年龄组外,各年龄组均数与男性总体均数之间比较差异无统计学意义(t=2.082,P=0.039;t=0.570,P=0.5790;t=0.156,P=0.876;t=-1.976,P=0.050;t=0.793,P=0.429);女性除20～29年龄组外,各年龄组均数与总体均数比较差异均有统计学意义(t=-2.065,P=0.002;t=-3.188,P=0.002;t=2.859,P=0.005;t=4.514,P<0.001)。结论血清尿酸浓度水平与年龄、性别有关,石家庄地区健康成人血清尿酸值有逐渐上升趋势,与现用正常参考值区间有一定差别,各实验室有必要建立自己的血清尿酸正常参考值区间并以此作为诊断的标准。