With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid...With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid-adult gut microbiota,and its association with host healthy are not well understood.In current study,we deemed to illustrate the associations of age,neuron dopamine,BAT remodeling,butyrate and gut microbiota with the aid of traditional herbal formula Kang Shuai Lao Pian(KSLP),which is known for its anti-aging effect.Here,ELISA was performed to detect the production of brain dopamine,the mass of inguinal white adipose tissue versus interscapular brown adipose tissue(iWAT/iBAT)was calculated and considered as a sign of BAT remodeling,16 S rRNA gene sequencing was used to the detection of gut microbiota profiling and gas chromatography was used to measure the butyrate level in mice feces.Our results indicated mid-adult mice already present distinctive gut microbiota profiling compared with young mice,concomitant with which are the lower brain dopamine level and disrupted brown adipose remodeling.KSLP treatment improved the host healthy and regulated gut microbiota with enriched Firmicutes at the expense of Bacteroidetes,particularly increased the relative abundance of bacteria functionally related to dopamine and butyrate productions,which suggest KSLP treatment constructs a healthier gut environment.In conclusion,modulation of gut microbiota and butyrate may connectively regulate dopamine production and BAT remodeling through gut-brain axis and gut-metabolism axis.展开更多
Background Chronic graft-versus-host disease （GVHD）,the commonest long-term complication after allogeneic hematopoietic stem cell transplantation （HSCT）,has a negative impact on patients＇ health related quality o...Background Chronic graft-versus-host disease （GVHD）,the commonest long-term complication after allogeneic hematopoietic stem cell transplantation （HSCT）,has a negative impact on patients＇ health related quality of life （HRQoL）.This study was designed to investigate the HRQoL in patients with chronic GVHD in China.Methods Two hundred and sixty-four patients with chronic GVHD who were ＞24 months post-HSCT and had been in continuous complete remission since HSCT were enrolled in this retrospective study.HRQoL was evaluated using an SF-36 questionnaire.Multivariate analysis was used to identify the factors that affect HRQoL in patients with chronic GVHD.Results HRQoL in patients categorized as having mild and moderate chronic GVHD was significantly better than in those in the severe category.In the moderate chronic GVHD category,markedly poorer HRQoL was observed in patients with both multiple organ involvement and more severe organ impairment than in those without these factors.According to multivariate analysis,chronic GVHD severity had the greatest significant negative impact on patients＇ HRQoL; whereas being female was associated with a negative impact on psychological health.Conclusion Chronic GVHD severity strongly correlates with negative impacts on patients＇ HRQoL.展开更多
基金supported by the Science and Technology Department of Zhejiang Province,China(Nos.2018C02048 and 2018F10076)the Agricultural and Social Development Department of Hangzhou City,China(2018)。
文摘With the occurrence of aging process,decreased neuron dopamine,disrupted brown adipose tissue(BAT)remodeling and decreased butyrate level all reflect a weak host healthy in certain degree.Nevertheless,the signs of mid-adult gut microbiota,and its association with host healthy are not well understood.In current study,we deemed to illustrate the associations of age,neuron dopamine,BAT remodeling,butyrate and gut microbiota with the aid of traditional herbal formula Kang Shuai Lao Pian(KSLP),which is known for its anti-aging effect.Here,ELISA was performed to detect the production of brain dopamine,the mass of inguinal white adipose tissue versus interscapular brown adipose tissue(iWAT/iBAT)was calculated and considered as a sign of BAT remodeling,16 S rRNA gene sequencing was used to the detection of gut microbiota profiling and gas chromatography was used to measure the butyrate level in mice feces.Our results indicated mid-adult mice already present distinctive gut microbiota profiling compared with young mice,concomitant with which are the lower brain dopamine level and disrupted brown adipose remodeling.KSLP treatment improved the host healthy and regulated gut microbiota with enriched Firmicutes at the expense of Bacteroidetes,particularly increased the relative abundance of bacteria functionally related to dopamine and butyrate productions,which suggest KSLP treatment constructs a healthier gut environment.In conclusion,modulation of gut microbiota and butyrate may connectively regulate dopamine production and BAT remodeling through gut-brain axis and gut-metabolism axis.
基金This work was partly supported by the National High Technology Research and Development Program of China （Program 863, No. 2011AA020105） and the National Natural Science Foundation of China （No. 30971292）.Acknowledgment： This manuscript has been edited by Lena R and Ryan L of American Journal Experts.
文摘Background Chronic graft-versus-host disease （GVHD）,the commonest long-term complication after allogeneic hematopoietic stem cell transplantation （HSCT）,has a negative impact on patients＇ health related quality of life （HRQoL）.This study was designed to investigate the HRQoL in patients with chronic GVHD in China.Methods Two hundred and sixty-four patients with chronic GVHD who were ＞24 months post-HSCT and had been in continuous complete remission since HSCT were enrolled in this retrospective study.HRQoL was evaluated using an SF-36 questionnaire.Multivariate analysis was used to identify the factors that affect HRQoL in patients with chronic GVHD.Results HRQoL in patients categorized as having mild and moderate chronic GVHD was significantly better than in those in the severe category.In the moderate chronic GVHD category,markedly poorer HRQoL was observed in patients with both multiple organ involvement and more severe organ impairment than in those without these factors.According to multivariate analysis,chronic GVHD severity had the greatest significant negative impact on patients＇ HRQoL; whereas being female was associated with a negative impact on psychological health.Conclusion Chronic GVHD severity strongly correlates with negative impacts on patients＇ HRQoL.
