[ Objective] This study was designed to explore whether heat-inactivated white spot syndrome virus （ WSSV ） could induce immune priming in Fenneropenaeus chinensis. [Method] Shrimps cultured at 15 （group E15℃ ）, ...[ Objective] This study was designed to explore whether heat-inactivated white spot syndrome virus （ WSSV ） could induce immune priming in Fenneropenaeus chinensis. [Method] Shrimps cultured at 15 （group E15℃ ）, 23 （group E23℃ ）, 28 （group E28℃ ） and 32 （group E32℃ ）℃ were fed for six successive days with the meat of WSSV-infected shrimps, which had been treated at 60℃ for 1 h. Meanwhile, the shrimps of positive control were fed with a diet containing active WSSV at 23℃ （ group C23℃ ）, and the shrimps of negative control were fed with commercial compound feed only at 23℃ （ group CF23℃ ）. On Day 13 of the experiment, the survivors were re-challenged with active WSSV, and then the survival time, survival rate, mortality rate and viral load in each group were measured. The results showed that no death occurred among the shrimps fed with a diet containing heat-inactivated WSSV before the secondary challenge, while all the shrimps fed with a diet containing active WSSV （ group CF23℃ ） died, suggesting that WSSV was completely inactivated by treatment at 60℃ for 1 h. On day 19 of the experiment, the survival rate in groups E15℃ , E23℃, E28℃, E32℃ and CF23℃ was 80.41% , 33.29%, 8.47% , 16.43% and 8.89%, respectively. There was an extremely significant difference in survival rate between group E15℃ and other groups （ P 〈 0.01 ）, a significant difference in survival rate between group E23℃ and CF23℃ , E28℃, E32℃ （P 〈 0. 05）, and no significant difference in survival rate between groups E28℃ and E32℃ （ P 〉 0. 05 ）. RT-qPCR revealed WSSV replicated most rapidly at 28℃ after the secondary challenge, while high （32℃） and low （ 15℃ ） temperature inhibited the multiplication of WSSV. In summary, heat-inactivated WSSV can induce immune priming response and provide some protection to shrimps against WSSV infection. The multiplication rate of WSSV is closely related to water temperature.展开更多
基金Supported by Central Public-interest Scientific Institution Basal Research Fund,Yellow Sea Fisheries Research Institute,CAFS(NO.20603022017001)National Natural Science Foundation of China(NO.31372523)+1 种基金The Taishan Scholar Program for Seed IndustryChina Agriculture Research System(CARS-48)
文摘[ Objective] This study was designed to explore whether heat-inactivated white spot syndrome virus （ WSSV ） could induce immune priming in Fenneropenaeus chinensis. [Method] Shrimps cultured at 15 （group E15℃ ）, 23 （group E23℃ ）, 28 （group E28℃ ） and 32 （group E32℃ ）℃ were fed for six successive days with the meat of WSSV-infected shrimps, which had been treated at 60℃ for 1 h. Meanwhile, the shrimps of positive control were fed with a diet containing active WSSV at 23℃ （ group C23℃ ）, and the shrimps of negative control were fed with commercial compound feed only at 23℃ （ group CF23℃ ）. On Day 13 of the experiment, the survivors were re-challenged with active WSSV, and then the survival time, survival rate, mortality rate and viral load in each group were measured. The results showed that no death occurred among the shrimps fed with a diet containing heat-inactivated WSSV before the secondary challenge, while all the shrimps fed with a diet containing active WSSV （ group CF23℃ ） died, suggesting that WSSV was completely inactivated by treatment at 60℃ for 1 h. On day 19 of the experiment, the survival rate in groups E15℃ , E23℃, E28℃, E32℃ and CF23℃ was 80.41% , 33.29%, 8.47% , 16.43% and 8.89%, respectively. There was an extremely significant difference in survival rate between group E15℃ and other groups （ P 〈 0.01 ）, a significant difference in survival rate between group E23℃ and CF23℃ , E28℃, E32℃ （P 〈 0. 05）, and no significant difference in survival rate between groups E28℃ and E32℃ （ P 〉 0. 05 ）. RT-qPCR revealed WSSV replicated most rapidly at 28℃ after the secondary challenge, while high （32℃） and low （ 15℃ ） temperature inhibited the multiplication of WSSV. In summary, heat-inactivated WSSV can induce immune priming response and provide some protection to shrimps against WSSV infection. The multiplication rate of WSSV is closely related to water temperature.
