Heat-sensitive moxibustion has neuroprotective effects against focal cerebral ischemia/reperfusion injury, however its mechanism of action remains unclear. In this study, rat models of focal cerebral ischemia/reperfus...Heat-sensitive moxibustion has neuroprotective effects against focal cerebral ischemia/reperfusion injury, however its mechanism of action remains unclear. In this study, rat models of focal cerebral ischemia/reperfusion injury were treated with suspended moxibustion at acupoint Dazhui （DU14） for 35 minutes. Results showed that suspended moxibustion decreased infarct volume, reduced cortical myeloperoxidase activity, and suppressed serum levels of proinflammatory cytokines in rats with focal cerebral ischemia/reperfusion injury. Our experimental findings indicated that heat-sensitive moxibustion can attenuate inflammation and promote repair after focal cerebral ischemia/reperfusion injury.展开更多
Ischemic stroke is universally acknowledged as a common cause of long-term disability or even death. Suspended moxibustion, an indirect form of moxibustion, is when moxibustion is placed superficially over the skin wi...Ischemic stroke is universally acknowledged as a common cause of long-term disability or even death. Suspended moxibustion, an indirect form of moxibustion, is when moxibustion is placed superficially over the skin without being in contact with it. Some researchers have used this method to treat stroke patients, but strong evidence of its therapeutic effectiveness is lacking. However, the effect of traditional suspended moxibustion has recently been improved with the development of heat-sensitive suspended moxibustion. Our previous studies showed that moxibustion for 35 min provided a more effective treatment strategy than moxibustion for 15 min, and moxibustion by 35 min with tail temperature increase had a better outcome than that without, however, the mechanism underlying the effect is not clear. In this study, we treated the stroke rats with moxibustion by 35min and divided them into non-heat sensitive moxibustion(NHSM) group and heat sensitive moxibustion (HSM) group according to difference in the tail temperature increase, then we compared the effect and investigated the mechanisms between NHSM and HSM. We found that HSM significantly decreased tail-flick latency, increased neurological function score, decreased infarct volume, reduced inflammatory cells, decreased the expression of inflammatory factor ICAM-1 and reduced the expression of NF-κB p65 and p-IKKα/β in rats with focal cerebral ischemia/reperfusion injury. Our experimental findings revealed that HSM exerted its anti-inflammatory and neuroprotective effects from MCAO-induced injury by decreasing the expression of the NF-κB signaling pathway.展开更多
Background:To explore the potential mechanism of thermal moxibustion in the treatment of i schemic strok e by using the method of network pharmacology and to partially explain the mechanism of acupoint meridian qi sen...Background:To explore the potential mechanism of thermal moxibustion in the treatment of i schemic strok e by using the method of network pharmacology and to partially explain the mechanism of acupoint meridian qi sense transmission.Methods:In this project,a training set was formed by researching the intersection of the reported components of M ugwort leaves that entered the blood after combustion and obtained the targets of heat-sensitive moxibustion meridian qi sensing and thermal effect by querying the literature.The Encyclopedia of Tr ad itional Chinese Medicine is used to search and screen the highly active chemical constituents of Artemisia argyi to predict potential targets;search i schemic strok e-related targets through GeneCards,O nline Mendelian Inheritance in Man,Drugbank databases.Gene Oncology functional analysis and Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment analysis were performed on the potential targets of the blood components of Artemisia argyi and molecular docking simulated the binding activity of key candidate active components and core genes.Results:192 ischemic stroke disease targets with r elevance score greater than 10 were screened out for analysis.Through the database Encyclopedia of Tr ad itional Chinese Medicine query of the action targets(243)of Mugwort leaves and the intersection of heat-sensitive moxibustion effect targets and ischemic stroke-related targets,a Venn diagram was drawn and a total of 17 were obtained.Finally,the effective components of Mugwort leaves into the blood were screened out.Five main volatile components were molecularly docked with 17 targets and 9 targets of acupoint meridian gas sensing/thermal effect.The docking results showed that the main volatile components showed good affinities for binding to key targets,respectively.Conclusion:Using the characteristics of“multi-component-multi-target-multi-pathway”of traditional Chinese medicine,the potential mechanism of action of Artemisia argyi in the treatment of i schemic strok e is explored,which provides a certain basis for the follow-up scientific research and clinical application of Artemisia argyi in the treatment of IS.展开更多
目的探讨参芪利水颗粒配合脐灸治疗慢性阻塞性肺疾病急性加重期(Acute exacerbation of chronic obstructivepulmonary disease,AECOPD)患者的临床疗效。方法选取2022年8月—2023年8月自河北省保定市第一中医院收治的60例AECOPD患者,按...目的探讨参芪利水颗粒配合脐灸治疗慢性阻塞性肺疾病急性加重期(Acute exacerbation of chronic obstructivepulmonary disease,AECOPD)患者的临床疗效。方法选取2022年8月—2023年8月自河北省保定市第一中医院收治的60例AECOPD患者,按照随机数字表法分为对照组和观察组,每组各30例。对照组采用常规西医治疗方法,观察组在对照组基础上脐灸联合参芪利水颗粒治疗,治疗2周后,观察比较两组患者临床疗效、治疗安全性,治疗前后改良版英国医学研究委员会呼吸问卷(modified British medical research council,mMRC)评分、慢阻肺患者自我评估测试(COPD Assessment test,CAT)评分、血气分析指标及血清炎性因子[降钙素原(Procalcitonin,PCT)、肿瘤坏死因子-α(Tumor necrosis factor alpha-α,TNF-α)、白细胞介素-6(Interleukin-6,IL-6)]水平。结果治疗后观察组临床总有效率93.33%(28/30)明显高于对照组66.67%(20/30),差异有统计学意义(P<0.05)。治疗后两组患者mMRC、CAT评分均较治疗前降低,差异有统计学意义(P<0.05);且观察组mMRC、CAT评分均明显低于对照组,差异有统计学意义(P<0.05)。治疗后两组患者PaCO_(2)指标均较治疗前降低,PaO_(2)和SaO_(2)指标均较治疗前升高,差异有统计学意义(P<0.05);且观察组PaCO_(2)指标较对照组明显降低,PaO_(2)和SaO 2指标均较对照组明显升高,差异有统计学意义(P<0.05)。治疗后两组患者PCT、TNF-α、IL-6水平均较治疗前降低,差异有统计学意义(P<0.05),且观察组PCT、TNF-α、IL-6水平均较对照组明显降低,差异有统计学意义(P<0.05)。治疗期间,两组患者均未见与治疗药物相关的不良反应情况发生。结论参芪利水颗粒配合脐灸能够抑制AECOPD炎症反应,改善患者血气分析指标,缓解临床症状。展开更多
We want to explore the analgesic brain effect of the moxibustion at heat-sensitized Yaoyangguan(GV3)in patients with lumbar disc herniation(LDH)and myofascial pain syndrome(MPS).In an assessor-blinded observational st...We want to explore the analgesic brain effect of the moxibustion at heat-sensitized Yaoyangguan(GV3)in patients with lumbar disc herniation(LDH)and myofascial pain syndrome(MPS).In an assessor-blinded observational study,we will include 15 LDH and 15 MPS.They will accept same treatment of heat-sensitive moxibustion at Yaoyangguan(GV3).The resting-state functionality magnetic resonance imaging image data of brain activities before and after treatment will be analyzed by mean fractional amplitude of low-frequency fluctuation,regional homogeneity analysis and brain functional connection.We select seed of first sensory cortex,second sensory cortex,insula cortex,periaqueductal gray and anterior cingulate cortex as the regions of interest to analyse the relationship between brain functional connectivity of pain-related networks and clinical data.Our study could disclose key brain targets and central response characteristics of the analgesic brain effect and the brain functional connection of heat-sensitive moxibustion.展开更多
目的探讨脐灸联合低频脉冲电治疗干预对脊髓损伤后神经源性膀胱的临床效果。方法选取2022年7月—2023年5月医院收治的60例脊髓损伤后神经源性膀胱患者为研究对象,按照组间基线资料均衡可比的原则分为对照组和观察组,各30例。对照组行常...目的探讨脐灸联合低频脉冲电治疗干预对脊髓损伤后神经源性膀胱的临床效果。方法选取2022年7月—2023年5月医院收治的60例脊髓损伤后神经源性膀胱患者为研究对象,按照组间基线资料均衡可比的原则分为对照组和观察组,各30例。对照组行常规康复训练、膀胱功能训练及间歇导尿护理干预;观察组在常规护理的基础上予以脐灸联合低频脉冲电治疗。观察两组患者的疗效;治疗前、治疗后2周、治疗结束时评估患者膀胱功能,指标包括日排尿次数、日漏尿次数、膀胱残余量及膀胱内最大压力;于治疗前、治疗后采用下尿路症状(Lower Urinary Tract Symptoms,LUTS)量表、焦虑自评量表(Self-Rating Anxiety Scale,SAS)及生活质量量表(Quality of Life Scale,QOL)评估患者当前症状、情绪状态及生活质量;比较两组患者不良反应发生率。结果两组患者日排尿次数、日漏尿次数及膀胱残余量、膀胱内压力比较,组间、不同时间点、组间与不同时间点交互作用,差异均有统计学意义(P<0.05)。各量表评分结果中,观察组患者治疗后LUTS得分、SAS得分均低于对照组,QOL得分则高于对照组,差异均有统计学意义(P<0.05)。两组患者不良反应发生率比较,差异无统计学意义(P>0.05)。结论脊髓损伤后神经源性膀胱患者应用脐灸联合低频脉冲电治疗可提高治疗效果,改善下尿路症状,促进膀胱功能恢复,进而利于缓解患者不良情绪、提高生活质量。展开更多
基金supported by the National Natural Science Foundation of China, No. 81060305
文摘Heat-sensitive moxibustion has neuroprotective effects against focal cerebral ischemia/reperfusion injury, however its mechanism of action remains unclear. In this study, rat models of focal cerebral ischemia/reperfusion injury were treated with suspended moxibustion at acupoint Dazhui （DU14） for 35 minutes. Results showed that suspended moxibustion decreased infarct volume, reduced cortical myeloperoxidase activity, and suppressed serum levels of proinflammatory cytokines in rats with focal cerebral ischemia/reperfusion injury. Our experimental findings indicated that heat-sensitive moxibustion can attenuate inflammation and promote repair after focal cerebral ischemia/reperfusion injury.
基金We would like to thank the National Natural Science Foun dation of China (81060305, 81660819)the Natural Science Foundation of Jiangxi Province (20151BAB205068), Key Project of Health Commission of Jiangxi province (2014Z003)Natural Science Foundation of Jiangxi University of Traditional Chinese Medicine (2014ZR018,- 2015jzzdxk024) for their supports.
文摘Ischemic stroke is universally acknowledged as a common cause of long-term disability or even death. Suspended moxibustion, an indirect form of moxibustion, is when moxibustion is placed superficially over the skin without being in contact with it. Some researchers have used this method to treat stroke patients, but strong evidence of its therapeutic effectiveness is lacking. However, the effect of traditional suspended moxibustion has recently been improved with the development of heat-sensitive suspended moxibustion. Our previous studies showed that moxibustion for 35 min provided a more effective treatment strategy than moxibustion for 15 min, and moxibustion by 35 min with tail temperature increase had a better outcome than that without, however, the mechanism underlying the effect is not clear. In this study, we treated the stroke rats with moxibustion by 35min and divided them into non-heat sensitive moxibustion(NHSM) group and heat sensitive moxibustion (HSM) group according to difference in the tail temperature increase, then we compared the effect and investigated the mechanisms between NHSM and HSM. We found that HSM significantly decreased tail-flick latency, increased neurological function score, decreased infarct volume, reduced inflammatory cells, decreased the expression of inflammatory factor ICAM-1 and reduced the expression of NF-κB p65 and p-IKKα/β in rats with focal cerebral ischemia/reperfusion injury. Our experimental findings revealed that HSM exerted its anti-inflammatory and neuroprotective effects from MCAO-induced injury by decreasing the expression of the NF-κB signaling pathway.
文摘Background:To explore the potential mechanism of thermal moxibustion in the treatment of i schemic strok e by using the method of network pharmacology and to partially explain the mechanism of acupoint meridian qi sense transmission.Methods:In this project,a training set was formed by researching the intersection of the reported components of M ugwort leaves that entered the blood after combustion and obtained the targets of heat-sensitive moxibustion meridian qi sensing and thermal effect by querying the literature.The Encyclopedia of Tr ad itional Chinese Medicine is used to search and screen the highly active chemical constituents of Artemisia argyi to predict potential targets;search i schemic strok e-related targets through GeneCards,O nline Mendelian Inheritance in Man,Drugbank databases.Gene Oncology functional analysis and Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment analysis were performed on the potential targets of the blood components of Artemisia argyi and molecular docking simulated the binding activity of key candidate active components and core genes.Results:192 ischemic stroke disease targets with r elevance score greater than 10 were screened out for analysis.Through the database Encyclopedia of Tr ad itional Chinese Medicine query of the action targets(243)of Mugwort leaves and the intersection of heat-sensitive moxibustion effect targets and ischemic stroke-related targets,a Venn diagram was drawn and a total of 17 were obtained.Finally,the effective components of Mugwort leaves into the blood were screened out.Five main volatile components were molecularly docked with 17 targets and 9 targets of acupoint meridian gas sensing/thermal effect.The docking results showed that the main volatile components showed good affinities for binding to key targets,respectively.Conclusion:Using the characteristics of“multi-component-multi-target-multi-pathway”of traditional Chinese medicine,the potential mechanism of action of Artemisia argyi in the treatment of i schemic strok e is explored,which provides a certain basis for the follow-up scientific research and clinical application of Artemisia argyi in the treatment of IS.
文摘目的探讨参芪利水颗粒配合脐灸治疗慢性阻塞性肺疾病急性加重期(Acute exacerbation of chronic obstructivepulmonary disease,AECOPD)患者的临床疗效。方法选取2022年8月—2023年8月自河北省保定市第一中医院收治的60例AECOPD患者,按照随机数字表法分为对照组和观察组,每组各30例。对照组采用常规西医治疗方法,观察组在对照组基础上脐灸联合参芪利水颗粒治疗,治疗2周后,观察比较两组患者临床疗效、治疗安全性,治疗前后改良版英国医学研究委员会呼吸问卷(modified British medical research council,mMRC)评分、慢阻肺患者自我评估测试(COPD Assessment test,CAT)评分、血气分析指标及血清炎性因子[降钙素原(Procalcitonin,PCT)、肿瘤坏死因子-α(Tumor necrosis factor alpha-α,TNF-α)、白细胞介素-6(Interleukin-6,IL-6)]水平。结果治疗后观察组临床总有效率93.33%(28/30)明显高于对照组66.67%(20/30),差异有统计学意义(P<0.05)。治疗后两组患者mMRC、CAT评分均较治疗前降低,差异有统计学意义(P<0.05);且观察组mMRC、CAT评分均明显低于对照组,差异有统计学意义(P<0.05)。治疗后两组患者PaCO_(2)指标均较治疗前降低,PaO_(2)和SaO_(2)指标均较治疗前升高,差异有统计学意义(P<0.05);且观察组PaCO_(2)指标较对照组明显降低,PaO_(2)和SaO 2指标均较对照组明显升高,差异有统计学意义(P<0.05)。治疗后两组患者PCT、TNF-α、IL-6水平均较治疗前降低,差异有统计学意义(P<0.05),且观察组PCT、TNF-α、IL-6水平均较对照组明显降低,差异有统计学意义(P<0.05)。治疗期间,两组患者均未见与治疗药物相关的不良反应情况发生。结论参芪利水颗粒配合脐灸能够抑制AECOPD炎症反应,改善患者血气分析指标,缓解临床症状。
基金Supported by Key Project of Jiangxi Provincial Youth Science Foundation:to Explore the Analgesic Mechanism of Brain Functional Network Regulation of moxibustion on Heat-sensitive Acupoints Based on rfMRI and MRS(No.20192ACB21007)Jiangxi Province Introduces and Cultivates Innovative and Entrepreneurial High-level Talent Projects:Brain Functional Network Regulation and Neurobiochemical Mechanism of Heat-sensitive Moxibustion Analgesia(No.jxsq2019201104)。
文摘We want to explore the analgesic brain effect of the moxibustion at heat-sensitized Yaoyangguan(GV3)in patients with lumbar disc herniation(LDH)and myofascial pain syndrome(MPS).In an assessor-blinded observational study,we will include 15 LDH and 15 MPS.They will accept same treatment of heat-sensitive moxibustion at Yaoyangguan(GV3).The resting-state functionality magnetic resonance imaging image data of brain activities before and after treatment will be analyzed by mean fractional amplitude of low-frequency fluctuation,regional homogeneity analysis and brain functional connection.We select seed of first sensory cortex,second sensory cortex,insula cortex,periaqueductal gray and anterior cingulate cortex as the regions of interest to analyse the relationship between brain functional connectivity of pain-related networks and clinical data.Our study could disclose key brain targets and central response characteristics of the analgesic brain effect and the brain functional connection of heat-sensitive moxibustion.
文摘目的探讨脐灸联合低频脉冲电治疗干预对脊髓损伤后神经源性膀胱的临床效果。方法选取2022年7月—2023年5月医院收治的60例脊髓损伤后神经源性膀胱患者为研究对象,按照组间基线资料均衡可比的原则分为对照组和观察组,各30例。对照组行常规康复训练、膀胱功能训练及间歇导尿护理干预;观察组在常规护理的基础上予以脐灸联合低频脉冲电治疗。观察两组患者的疗效;治疗前、治疗后2周、治疗结束时评估患者膀胱功能,指标包括日排尿次数、日漏尿次数、膀胱残余量及膀胱内最大压力;于治疗前、治疗后采用下尿路症状(Lower Urinary Tract Symptoms,LUTS)量表、焦虑自评量表(Self-Rating Anxiety Scale,SAS)及生活质量量表(Quality of Life Scale,QOL)评估患者当前症状、情绪状态及生活质量;比较两组患者不良反应发生率。结果两组患者日排尿次数、日漏尿次数及膀胱残余量、膀胱内压力比较,组间、不同时间点、组间与不同时间点交互作用,差异均有统计学意义(P<0.05)。各量表评分结果中,观察组患者治疗后LUTS得分、SAS得分均低于对照组,QOL得分则高于对照组,差异均有统计学意义(P<0.05)。两组患者不良反应发生率比较,差异无统计学意义(P>0.05)。结论脊髓损伤后神经源性膀胱患者应用脐灸联合低频脉冲电治疗可提高治疗效果,改善下尿路症状,促进膀胱功能恢复,进而利于缓解患者不良情绪、提高生活质量。
