[Objectives]The purpose was to establish an induction system for friable callus of Hedera nepalensis var.sinensis with different parts.[Methods]By screening the most suitable explant and adjusting the hormone ratio of...[Objectives]The purpose was to establish an induction system for friable callus of Hedera nepalensis var.sinensis with different parts.[Methods]By screening the most suitable explant and adjusting the hormone ratio of medium,friable calli of H.nepalensis var.sinensis were induced.[Results]The calli could be induced from leaves,petioles and stem segments,but the ideal explant was stem segments,with induction rate reaching 98%.The optimal medium for callus proliferation was MS+0.5 mg/L KT+1.0 mg/L 2,4-D+30.0 g/L sucrose.After 3-4 generations of subculture on MS+0.5 mg/L BA+1.0 mg/L 2,4-D+30.0 g/L sucrose,favorable friable calli of H.nepalensis var.sinensis were obtained.[Conclusions]The friable calli induced in this experiment can lay a foundation for in-vitro regeneration and cellular secondary metabolite production of H.nepalensis var.sinensis.展开更多
基金Tianjin Science and Technology Plan Project(18ZXBFNC00370)Industrial Innovation and Entrepreneurship Team Project of Hebei Province(199A2905H)Fund of Central Government for Guiding Science and Technology Development in Hebei Province(206Z6303G).
文摘[Objectives]The purpose was to establish an induction system for friable callus of Hedera nepalensis var.sinensis with different parts.[Methods]By screening the most suitable explant and adjusting the hormone ratio of medium,friable calli of H.nepalensis var.sinensis were induced.[Results]The calli could be induced from leaves,petioles and stem segments,but the ideal explant was stem segments,with induction rate reaching 98%.The optimal medium for callus proliferation was MS+0.5 mg/L KT+1.0 mg/L 2,4-D+30.0 g/L sucrose.After 3-4 generations of subculture on MS+0.5 mg/L BA+1.0 mg/L 2,4-D+30.0 g/L sucrose,favorable friable calli of H.nepalensis var.sinensis were obtained.[Conclusions]The friable calli induced in this experiment can lay a foundation for in-vitro regeneration and cellular secondary metabolite production of H.nepalensis var.sinensis.
