Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the curren...Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the current system was ineffective.Methods Microscopy was used to observe the characteristics of the powder and cross-sectional tissue.Thin layer chromatography(TLC)was employed to separate HC from Hedyotis diffusa(Bai Hua She She Cao,白花蛇舌草,HD).Simultaneously,high-performance liquid chromatography(HPLC)was used to establish the characteristic spectrum of HC and determine the concentrations of hedyotiscone A.Regarding the issue of systematicness,concentrations of water,total ash,acid-insoluble ash,heavy metals(including lead,arsenic,mercury,cadmium and copper)and pesticide residue were measured by the methods described in the 2015 edition of Chinese Pharmacopoeia.Results Calcium oxalate needle crystal bundles and oil drops were observed in the cross-sectional tissue.Three characteristic blue fluorescent spots were observed in the HC samples by TLC.There were five distinct characteristic peaks in HC but not in HD,and hedyotiscone A was the sharpest characteristic peak.The generated linear equation of hedyotiscone A was Y=3.15×108X+312.04(r2=1.000)between 0.00005270 and 0.002108 mg/mL,and the average recovery was 100.42%.Precision[relative standard deviation(RSD)0.92%],repeatability(RSD 0.14%),and recovery(RSD 0.85%)met the requirements for quantitative analysis.Hedyotiscone A concentrations ranged from 0.00017%~0.00113%across different habitats.Concentrations of water,ash and acid-insoluble ash were 8.80%~11.2%,7.00%~15.3%and 0.440%~7.70%,respectively.Concentrations of Pb,As,Hg,Cd and Cu were 1.60~4.60,0.650~1.60,0.0300~0.100,0.240~0.690 and 10.1~16.6μg/g,respectively.Pesticide residues were less than 0.00100 ppm.Conclusion The quality control system can effectively distinguish HC from HD,so as to comprehensively control the quality of HC,to provide references for the clinical use of HC.展开更多
Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabi...Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.展开更多
目的 研究茜草科植物红芽大戟 (KnoxiacorymbosaWilld .)的化学成分。方法 利用硅胶、聚酰胺等色谱技术分离纯化 ,根据化合物的理化性质和光谱数据进行鉴定。结果 从红芽大戟的正丁醇萃取部分分离得到 4个黄酮醇苷成分 ,分别鉴定为 ...目的 研究茜草科植物红芽大戟 (KnoxiacorymbosaWilld .)的化学成分。方法 利用硅胶、聚酰胺等色谱技术分离纯化 ,根据化合物的理化性质和光谱数据进行鉴定。结果 从红芽大戟的正丁醇萃取部分分离得到 4个黄酮醇苷成分 ,分别鉴定为 :槲皮素 7 O α L 阿拉伯糖 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 7 O α L arabinosyl 3 O β D 6″ acetylglucopyranoside ,1 ) ;山奈酚 7 O α L 阿拉伯糖 3 O β D 吡喃葡糖苷 (kaempferol 7 O α L arabinosyl 3 O β D glucopyranoside ,2 ) ;槲皮素 3 O β D 吡喃葡糖苷 (quercetin 3 O β D glucopyranoside ,3) ;槲皮素 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 3 O β D 6″ acetylglucopyranoside,4 )。 结论 化合物 1为新化合物 。展开更多
基金support from the National Science foundation of China(No.81503041)the Science Research Projects of Chinese Pharmacopoeia(No.Z18)+2 种基金the Science Research Projects of the Hunan Provincial Department of Education(No.17C1213)the Science Research Projects of the Hunan Provincial Department of Education(No.14C0860)and the Key Projects of the Changsha Municipal Science and Technology Bureau(No.K1406030-31)
文摘Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the current system was ineffective.Methods Microscopy was used to observe the characteristics of the powder and cross-sectional tissue.Thin layer chromatography(TLC)was employed to separate HC from Hedyotis diffusa(Bai Hua She She Cao,白花蛇舌草,HD).Simultaneously,high-performance liquid chromatography(HPLC)was used to establish the characteristic spectrum of HC and determine the concentrations of hedyotiscone A.Regarding the issue of systematicness,concentrations of water,total ash,acid-insoluble ash,heavy metals(including lead,arsenic,mercury,cadmium and copper)and pesticide residue were measured by the methods described in the 2015 edition of Chinese Pharmacopoeia.Results Calcium oxalate needle crystal bundles and oil drops were observed in the cross-sectional tissue.Three characteristic blue fluorescent spots were observed in the HC samples by TLC.There were five distinct characteristic peaks in HC but not in HD,and hedyotiscone A was the sharpest characteristic peak.The generated linear equation of hedyotiscone A was Y=3.15×108X+312.04(r2=1.000)between 0.00005270 and 0.002108 mg/mL,and the average recovery was 100.42%.Precision[relative standard deviation(RSD)0.92%],repeatability(RSD 0.14%),and recovery(RSD 0.85%)met the requirements for quantitative analysis.Hedyotiscone A concentrations ranged from 0.00017%~0.00113%across different habitats.Concentrations of water,ash and acid-insoluble ash were 8.80%~11.2%,7.00%~15.3%and 0.440%~7.70%,respectively.Concentrations of Pb,As,Hg,Cd and Cu were 1.60~4.60,0.650~1.60,0.0300~0.100,0.240~0.690 and 10.1~16.6μg/g,respectively.Pesticide residues were less than 0.00100 ppm.Conclusion The quality control system can effectively distinguish HC from HD,so as to comprehensively control the quality of HC,to provide references for the clinical use of HC.
文摘Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.
文摘目的 研究茜草科植物红芽大戟 (KnoxiacorymbosaWilld .)的化学成分。方法 利用硅胶、聚酰胺等色谱技术分离纯化 ,根据化合物的理化性质和光谱数据进行鉴定。结果 从红芽大戟的正丁醇萃取部分分离得到 4个黄酮醇苷成分 ,分别鉴定为 :槲皮素 7 O α L 阿拉伯糖 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 7 O α L arabinosyl 3 O β D 6″ acetylglucopyranoside ,1 ) ;山奈酚 7 O α L 阿拉伯糖 3 O β D 吡喃葡糖苷 (kaempferol 7 O α L arabinosyl 3 O β D glucopyranoside ,2 ) ;槲皮素 3 O β D 吡喃葡糖苷 (quercetin 3 O β D glucopyranoside ,3) ;槲皮素 3 O β D 6″ 乙酰基吡喃葡糖苷 (quercetin 3 O β D 6″ acetylglucopyranoside,4 )。 结论 化合物 1为新化合物 。
