Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(...Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(%)were calculated by Celltiter-GLO Assay for evaluating CC50,EC50 and SI values.To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity,western-blot was used to detect the protein expression levels of NS5A,NS3 and NS5B.Results:the CC50,EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml,1.90g/ml and 67.90 respectively.The EC50 after 24h,48h and 72h administration were 18g/ml,5.8g/ml and 2.3g/ml respectively.Within the range of drug concentration,the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship,but had no obvious effect on the expression of NS3 protein.Conclusion:The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B,and the virus has no tolerance to the aqueous extract of Hehuan Yin.展开更多
基金Natural Foundation of Guangdong Province(No.2018A030313308)。
文摘Objective:To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract.Methods:Huh7.5.1 cells were used to establish the HCV2a virus infection model.Cell survival rate(%)and Renilla Luciferase Assay Kit(%)were calculated by Celltiter-GLO Assay for evaluating CC50,EC50 and SI values.To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity,western-blot was used to detect the protein expression levels of NS5A,NS3 and NS5B.Results:the CC50,EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml,1.90g/ml and 67.90 respectively.The EC50 after 24h,48h and 72h administration were 18g/ml,5.8g/ml and 2.3g/ml respectively.Within the range of drug concentration,the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship,but had no obvious effect on the expression of NS3 protein.Conclusion:The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B,and the virus has no tolerance to the aqueous extract of Hehuan Yin.
文摘目的:探讨解郁合欢膏治疗卒中后抑郁(Post-stroke Depression,PSD)的临床疗效。方法:选择2018年7月1日-2019年6月30日在南宁市中医医院脑病科门诊及住院的PSD患者96例作为研究对象,以随机数字表法简单随机分组为两组,剔除脱落后对照组45例,观察组46例。对照组在常规治疗基础上口服盐酸帕罗西汀,观察组口服解郁合欢膏,疗程均为8周,分别于治疗前、治疗后使用汉密尔顿抑郁量表(Hamilton Depression Scale,HAMD)、改良Bathel指数(Barthel Index,BI)、美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评定疗效,以及不良反应发生情况。结果:治疗后观察组总有效率高于对照组,两组患者HAMD评分、NIHSS评分比较均低于治疗前(P<0.05),BI评分高于治疗前(P<0.05),且观察组优于对照组,差异有统计学意义(P<0.05)。两组均未发生严重药物不良反应。结论:解郁合欢膏治疗卒中后抑郁可改善患者抑郁症状,提高生活质量,有较好的治疗效果,且患者不良反应发生率较低。
