The developmental genetics of plant height was analyzed from two groups of three-line indica hybrid rice at two environmental conditions based on the NCII design, using the additive-dominant developmental genetics mod...The developmental genetics of plant height was analyzed from two groups of three-line indica hybrid rice at two environmental conditions based on the NCII design, using the additive-dominant developmental genetics models and the statistic methods. The results showed that the rice genotypes and environmental conditions could both affect plant height, and the effects of environment on plant height decreased gradually with plant development. Additive and dominant effects both governed the performance of plant height at all developmental stages. However, the degrees of effect varied among the rice genotypes. Moreover, the interaction between environments and genotypes also affected plant height. The genetic effects differed at most developmental stages. Furthermore, the expressJon of additive effect was more active than that of dominant effect. Conditional interaction effects with environment also influenced plant height during genetic development, especially at the eady stage. Mid-parent heterosis （HMP） increased gradually with the developmental stage of plant height, and maximized at the latest stage, whereas the heterosis over the better parent （HBP） showed small differences among the genotypes, and kept stable at the later stage, with positive numeric value. At most developmental stages, conditional HMP was positively significant, while conditional HBP was negatively significant. All above results suggest that HMP and HBP have some new expressions in all developmental periods and the levels and directions are quite different.展开更多
Plant height （PH） is one of the most important agronomic traits of rice, as it directly affects the lodging resistance and the high yield potential. Meanwhile, PH is often constrained by water supply over the entire...Plant height （PH） is one of the most important agronomic traits of rice, as it directly affects the lodging resistance and the high yield potential. Meanwhile, PH is often constrained by water supply over the entire growth period. In this study, a recombinant inbred line （RIL） derived from Xiaobaijingzi and Kongyu 131 strains grown under drought stress and with normal irrigation over 2 yr （2013 and 2014）, respectively （regarded as four environments）, was used to dissect the genetic basis of PH by developmental dynamics QTL analysis combined with QTL^environment interactions. QTLs with net effects excluding the accumulated effects were detected to explore the relationship between genexgene interactions and genexenvironment interactions in specific growth period. A total of 26 additive QTLs （A-QTLs） and 37 epistatic QTLs （E-QTLs） associated with PH were detected by unconditional and conditional mapping over seven growth periods, qPH-2-3, qPH-4-3, qPH-6-1, qPH-7-1, and qPH-12-5 could be detected by both unconditional and conditional analyses, qPH-4-3 and qPH-7-5 were detected in four stages （periods） to be sequentially expressed QTLs controlling PH continuous variation. QTLs with additive effects （A-QTLs） were mostly expressed in the period $3iS2 （the time interval from stages 2 to 3）, and QTLxenvironment interactions performed actively in the first three stages （periods） which could be an important developmental period for rice to undergo external morphogenesis during drought stress. Several QTLs showed high adaptability for drought stress and many QTLs were closely related to the environments such as qPH-3-5, qPH-2-2 and qPH-6-1. 72.5% of the QTLs with a and aa effects detected by conditional analysis were under drought stress, and the PVE of QTLs detected by conditional analysis under drought stress were also much higher than that under normal irrigation. We infer that environments would influence the detection results and sequential expression of genes was highly influenced by environments as well. Many QTLs （qPH-1-2, qPH-3-5, qPH-4-1, qPH-2-3） coincident with previously identified drought resistance genes. The result of this study is helpful to elucidating the genetic mechanism and regulatory network underlying the development of PH in rice and providing references to marker assisted selection.展开更多
为了检测盐胁迫下水稻苗高和分蘖数的发育动态QTL,以粳稻品种东农425和长白10为亲本衍生的F2:3群体为试验材料,构建了包含123个SSR标记,全长为1 616.53 c M,平均图距为13.14 c M的遗传连锁图谱。以浓度为6 ds·m-1的Na Cl水溶液进...为了检测盐胁迫下水稻苗高和分蘖数的发育动态QTL,以粳稻品种东农425和长白10为亲本衍生的F2:3群体为试验材料,构建了包含123个SSR标记,全长为1 616.53 c M,平均图距为13.14 c M的遗传连锁图谱。以浓度为6 ds·m-1的Na Cl水溶液进行大田生育期灌溉,正常水灌溉为对照,对盐胁迫下水稻的苗高和分蘖数进行发育动态QTL分析。分别利用完备区间作图法和混合线性模型的QTL定位方法,联合盐胁迫与正常条件下6个发育时期苗高和分蘖数的表型数据,共检测到6个控制盐胁迫下水稻苗高和3个控制分蘖数的加性QTL、4个控制正常条件下苗高和5个控制分蘖数的加性QTL、盐胁迫和正常条件联合下的6个控制苗高和4个控制分蘖数的加性QTL,以及3对控制苗高和1对控制分蘖数的上位性QTL。加性QTL q SH1在t3、t4和t2/t1时期分别用非条件和条件方法检测到,加性QTL q TN8-2在t2、t3、t4和t5时期被连续用非条件方法检测到,在t3/t2时期用条件方法被检测到。分别检测到4个控制苗高和2个控制分蘖数的加性QTL与盐胁迫环境存在互作效应,控制苗高的3对上位性QTL和控制分蘖数的1对上位性QTL均与盐胁迫环境发生互作。本研究旨在检测不同发育时期控制盐胁迫下水稻苗高和分蘖数的QTL,并分析与盐环境的互作效应,为解析苗高和分蘖数在盐胁迫下的发育遗传特点和水稻耐盐QTL分子标记辅助育种提供理论依据。展开更多
目的分析高脱位髋关节发育不良(developmental dysplasia of hip,DDH)患者的身高与真臼大小、形态及后壁骨量的相关性。方法收集2014年4月-2015年5月15例(21髋)Crowe分型为Ⅳ型且继发髋关节骨性关节炎于我院行人工髋关节置换术(total hi...目的分析高脱位髋关节发育不良(developmental dysplasia of hip,DDH)患者的身高与真臼大小、形态及后壁骨量的相关性。方法收集2014年4月-2015年5月15例(21髋)Crowe分型为Ⅳ型且继发髋关节骨性关节炎于我院行人工髋关节置换术(total hip arthroplasty,THA)治疗的DDH患者,男2例2髋,女13例19髋,平均年龄(25±7.2)岁。术前行骨盆正位X线和髋臼及股骨中上段CT扫描,分析患者身高与骨盆高度、真臼前后径宽度、深度、内壁厚度和后壁骨量的相关性。结果 CroweⅣ型DDH患者身高与骨盆高度呈正相关(r=0.723,P=0.000);身高与真臼前后径(r=0.049,P=0.834)、内壁厚度(r=0.395,P=0.076)、深度(r=0.205,P=0.373)、后壁骨量(r=0.152,P=0.511)、髋臼假体直径(r=0.010,P=0.966)均无相关性。结论 CroweⅣ型DDH患者的身高与骨盆高度呈正相关,但与真臼大小、形态及后壁骨量无相关性,身高、体型不同的患者真臼形态差异小。展开更多
基金supported by the National Natural Science Foundation of China(Grant No. 30370865) and the Innovation Projects of New Plant and Animal Breeding in Chongqing Municipality, China
文摘The developmental genetics of plant height was analyzed from two groups of three-line indica hybrid rice at two environmental conditions based on the NCII design, using the additive-dominant developmental genetics models and the statistic methods. The results showed that the rice genotypes and environmental conditions could both affect plant height, and the effects of environment on plant height decreased gradually with plant development. Additive and dominant effects both governed the performance of plant height at all developmental stages. However, the degrees of effect varied among the rice genotypes. Moreover, the interaction between environments and genotypes also affected plant height. The genetic effects differed at most developmental stages. Furthermore, the expressJon of additive effect was more active than that of dominant effect. Conditional interaction effects with environment also influenced plant height during genetic development, especially at the eady stage. Mid-parent heterosis （HMP） increased gradually with the developmental stage of plant height, and maximized at the latest stage, whereas the heterosis over the better parent （HBP） showed small differences among the genotypes, and kept stable at the later stage, with positive numeric value. At most developmental stages, conditional HMP was positively significant, while conditional HBP was negatively significant. All above results suggest that HMP and HBP have some new expressions in all developmental periods and the levels and directions are quite different.
基金supported by the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD20B04)
文摘Plant height （PH） is one of the most important agronomic traits of rice, as it directly affects the lodging resistance and the high yield potential. Meanwhile, PH is often constrained by water supply over the entire growth period. In this study, a recombinant inbred line （RIL） derived from Xiaobaijingzi and Kongyu 131 strains grown under drought stress and with normal irrigation over 2 yr （2013 and 2014）, respectively （regarded as four environments）, was used to dissect the genetic basis of PH by developmental dynamics QTL analysis combined with QTL^environment interactions. QTLs with net effects excluding the accumulated effects were detected to explore the relationship between genexgene interactions and genexenvironment interactions in specific growth period. A total of 26 additive QTLs （A-QTLs） and 37 epistatic QTLs （E-QTLs） associated with PH were detected by unconditional and conditional mapping over seven growth periods, qPH-2-3, qPH-4-3, qPH-6-1, qPH-7-1, and qPH-12-5 could be detected by both unconditional and conditional analyses, qPH-4-3 and qPH-7-5 were detected in four stages （periods） to be sequentially expressed QTLs controlling PH continuous variation. QTLs with additive effects （A-QTLs） were mostly expressed in the period $3iS2 （the time interval from stages 2 to 3）, and QTLxenvironment interactions performed actively in the first three stages （periods） which could be an important developmental period for rice to undergo external morphogenesis during drought stress. Several QTLs showed high adaptability for drought stress and many QTLs were closely related to the environments such as qPH-3-5, qPH-2-2 and qPH-6-1. 72.5% of the QTLs with a and aa effects detected by conditional analysis were under drought stress, and the PVE of QTLs detected by conditional analysis under drought stress were also much higher than that under normal irrigation. We infer that environments would influence the detection results and sequential expression of genes was highly influenced by environments as well. Many QTLs （qPH-1-2, qPH-3-5, qPH-4-1, qPH-2-3） coincident with previously identified drought resistance genes. The result of this study is helpful to elucidating the genetic mechanism and regulatory network underlying the development of PH in rice and providing references to marker assisted selection.
文摘目的分析高脱位髋关节发育不良(developmental dysplasia of hip,DDH)患者的身高与真臼大小、形态及后壁骨量的相关性。方法收集2014年4月-2015年5月15例(21髋)Crowe分型为Ⅳ型且继发髋关节骨性关节炎于我院行人工髋关节置换术(total hip arthroplasty,THA)治疗的DDH患者,男2例2髋,女13例19髋,平均年龄(25±7.2)岁。术前行骨盆正位X线和髋臼及股骨中上段CT扫描,分析患者身高与骨盆高度、真臼前后径宽度、深度、内壁厚度和后壁骨量的相关性。结果 CroweⅣ型DDH患者身高与骨盆高度呈正相关(r=0.723,P=0.000);身高与真臼前后径(r=0.049,P=0.834)、内壁厚度(r=0.395,P=0.076)、深度(r=0.205,P=0.373)、后壁骨量(r=0.152,P=0.511)、髋臼假体直径(r=0.010,P=0.966)均无相关性。结论 CroweⅣ型DDH患者的身高与骨盆高度呈正相关,但与真臼大小、形态及后壁骨量无相关性,身高、体型不同的患者真臼形态差异小。
