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Heirlooms番茄果实叶绿素和可溶性固形物含量的聚类分析 被引量:2
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作者 唐亚萍 杨涛 +7 位作者 杨生保 李宁 许娟 帕提古丽 王柏柯 张贵仁 Ann Powell 余庆辉 《新疆农业科学》 CAS CSCD 北大核心 2017年第6期1021-1028,共8页
【目的】对25个栽培番茄Heirlooms和3个野生番茄品种进行田间表型多样性分析,对果实不同部位叶绿素含量和成熟果实可溶性固形物含量进行检测及聚类分析。【方法】分别在小果期和中果期利用分光光度法,检测番茄果实上部和下部叶绿素含量... 【目的】对25个栽培番茄Heirlooms和3个野生番茄品种进行田间表型多样性分析,对果实不同部位叶绿素含量和成熟果实可溶性固形物含量进行检测及聚类分析。【方法】分别在小果期和中果期利用分光光度法,检测番茄果实上部和下部叶绿素含量的差异,运用折光仪检测番茄果实3个发育时期的可溶性固形物的含量并进行聚类分析。【结果】3个野生番茄与栽培番茄Heirlooms品系在叶绿素和可溶性固形物方面具有明显的差异,并通过聚类分析与Heirloom品系区分。在Heirloom品系中,叶绿素含量在含有果肩的部分明显高于远离果肩的底部,果实品质相关的可溶性固形物的含量与番茄果实果肩部分叶绿素含量表现基本一致。可溶性固形物含量最高为7.07%的Heirloom品系中的樱桃番茄Yellow Vernissage。【结论】具有正常果型的Bali可溶性固形物含量为6.76%,以及具有6.13%可溶性固形物的大果型Big Zebra,均可以用于满足不同果形的番茄品质提高的育种研究应用。 展开更多
关键词 Heirloom品系 果实叶绿素 可溶性固形物 聚类分析
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SlGLK2转录因子在番茄品种Heirloom果实中的功能及变异分析 被引量:1
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作者 唐亚萍 杨涛 +3 位作者 帕提古丽 王柏柯 杨生保 余庆辉 《北方园艺》 CAS 北大核心 2019年第11期24-32,共9页
Goldern2-Like(SlGLK2)基因是调控未成熟果实叶绿体发育重要的转录因子。番茄(Solanum lycopersicum)未成熟果实中叶绿体对成熟果实的糖类、风味物质积累和提高番茄品质具有一定的作用。Heirloom品系作为优异的种质资源,在果实颜色、大... Goldern2-Like(SlGLK2)基因是调控未成熟果实叶绿体发育重要的转录因子。番茄(Solanum lycopersicum)未成熟果实中叶绿体对成熟果实的糖类、风味物质积累和提高番茄品质具有一定的作用。Heirloom品系作为优异的种质资源,在果实颜色、大小、形状和风味等方面有很大差异。以32个Heirloom品种为研究材料,通过表型调查研究果实表型性状,检测果实不同部位和不同发育时期叶绿素含量,筛选20个Heirloom品种克隆SlGLK2基因和启动子序列,运用荧光定量PCR分析调控果实叶绿体发育的相关基因,从而分析基因间的调控关系,以期研究SlGLK2转录因子对Heirloom品种叶绿体发育的影响。结果表明:含有SlGLK2基因的品种,未成熟果实拥有深绿色果肩,其不同发育时期绿色果实果肩的叶绿素含量明显高于果实底部。在SlGLK2基因上游非编码区和编码区共发现21个单核苷酸多态性(SNPs),经聚类分析发现,部分SNPs与未成熟果实的表型相关。果实叶绿体发育相关基因的表达分析表明TKN2、TKN4和在番茄果实的果肩部表达量明显高于果实底部,且TKN2、TKN4位于SlGLK2上游并调控SlGLK2基因的表达。 展开更多
关键词 heirlooms SlGLK2 叶绿素 单核苷酸多态性(SNPs)
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Evaluation of Heirloom and Commercial Cultivars of Small Grains under Low Input Organic Systems
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作者 Tejendra Chapagain Andrew Riseman 《American Journal of Plant Sciences》 2012年第5期655-669,共15页
A cultivar trial, including commercial and heirloom cultivars of major cereals and grain legumes was conducted in Vancouver, BC, under low input organic conditions. We assessed 19 wheat (6 commercial and 13 heirloom),... A cultivar trial, including commercial and heirloom cultivars of major cereals and grain legumes was conducted in Vancouver, BC, under low input organic conditions. We assessed 19 wheat (6 commercial and 13 heirloom), 17 barley (8 commercial and 9 heirloom), 5 pea, 5 favabean, 5 kidneybean, 2 lentil, and 2 soyabean cultivars for plant performance metrics, and their potential in a small grain:legume intercropping system. Heirloom wheat cultivars showed notable response in a number of parameters including late maturity, taller plants, greatest number of spikes per m2, longest spike, highest number of seed per spike, greater seed weight to volume ratio, and resistance to stripe rust compared with commercial cultivars. For the heirloom-type, 6 of 14 wheat cultivars, (i.e., “Reward”, “Glenn”, “Cerebs”, “Red Bobs”, “Sounders” and “Black Bearded”) produced yields comparable to the commercial cultivars (i.e., nearly 5 t/h or higher). Also, heirloom cultivars typically contained higher protein levels most suitable for baking and blending purposes with “Einkorn” displaying the highest level (16.2%). Heirloom and commercial barley cultivars did not differ significantly with respect to plant height, spike length, and seed weight to volume ratio. However, a number of heirloom cultivars (e.g., “Jet”, “Dolma”, “Andie” and “Himalayan”) displayed greater responses on earliness, number of spikes per m2, grain yield, protein content and seed weight to volume ratio. Pea and lentil yielded lower than the national average under trial conditions. However, heirloom peas “Corgi”, “De Grace”, “Snowbird”, and “Golden” were earlier compared to the commercial cultivar “Reward”. All kidney bean cultivars yielded ~3 t/h with the highest yield from “Red Kidney” (3.8 t/h). Fava and soyabean appeared as promising crops as the cultivars produced good growth and yields. Neither lentil (“Crimson” and “Essex”) produced satisfactory responses though they had excellent vegetative growth and flowered. Therefore, significant variation was observed including several heirloom cultivars displayed great potential in terms of yield, protein content, and disease resistance and that specific cultivars were better suited for an intercropping system. 展开更多
关键词 COMMERCIAL and Heirloom CULTIVARS Hulled and Hulless 2-Row and 6-Row INTERCROPPING
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