期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Evaluating the immune responses of mice to subcutaneous immunization with Helicobacter pylori urease B subunit 被引量:5
1
作者 Peng Sun Jia-Qi Wang +1 位作者 Yu-Tao Zhang Sheng-Guo Zhao 《Journal of Animal Science and Biotechnology》 SCIE CAS 2014年第2期181-187,共7页
Background: Helicobacter pylori, a gram-negative bacterial pathogen that expresses a strong urease activity, is associated with the development of gastroduodenal disease. Urease B subunit, one of the two structural s... Background: Helicobacter pylori, a gram-negative bacterial pathogen that expresses a strong urease activity, is associated with the development of gastroduodenal disease. Urease B subunit, one of the two structural subunits of urease, was expressed in E. coil BL21 (DE3) strain. The objective of this study was to evaluate the effects of He/icobacter py/ori urease B subunit on the immune responses in mice by subcutaneous immunization. Methods: The mice were immunized and boosted with Helicobacter pylori urease B subunit antigen subcutaneously three times with 2-wk intervals between the immunizations and boosters. The mice in the control group were immunized with PBS. The adjuvant group received PBS containing complete/incomplete freund's adjuvant identical to antigen group without Helicobocter pylori urease B subunit antigen. Four weeks after the final booster, all the mice were sacrificed. Blood was collected on d 0, 14, 28 and 56 before immunization, booster and sacrifice, respectively. Immediately after sacrifice, gastric liquid and spleen were collected for antibody and cytokine analyses. Results: Urease B subunit increased the concentrations of serum and gastric anti-urease B antigen specific IgG, and the levels of interteukin-4 and interferon-y in splenocytes of the mice (P 〈 0.05). Conclusions: This study demonstrated that recombinant responses in mice by subcutaneous immunization, which against Helicobocter pylori. urease B subunit can induce systemic and local immune might be used as the effective component of vaccine 展开更多
关键词 ANTIbODY CYTOKINES helicobacter pylori urease b subunit MICE
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部