Introduction: Gastroduodenal lesions are common in chronic kidney disease (CKD). They are linked to various factors including Helicobacter pylori infection (H. pylori). Few data are available in Africa on H. pylori in...Introduction: Gastroduodenal lesions are common in chronic kidney disease (CKD). They are linked to various factors including Helicobacter pylori infection (H. pylori). Few data are available in Africa on H. pylori infection and chronic kidney disease. The aim of this study was to assess the impact of H. pylori infection and to describe the gastroduodenal lesions found in patients with chronic kidney disease. Patients and Methods: A cross-sectional study was conducted, February 1<sup>st</sup> to May 31<sup>st</sup>, 2021, at the Douala General Hospital in Cameroon. We included patients with CKD classified as stages 3 to 5 according KDIGO classification, on hemodialysis or not, who agreed to participate in the study. They were matched with a “control” population including patients with normal renal function according to sex and age (ratio 1:2). Patients on antibiotics and/or proton pump inhibitors were excluded. We collected data from CKD patients and from medical records for non-CKD group. Each patient underwent an upper digestive endoscopy and identification of H. pylori using a urease rapid test. Logistic regression was used to identify independent associations for a significance level set at p Results: We included 99 patients including 33 with CKD and 66 control patients. Among patients with CKD, the predominance was male (n = 18/33 or 54.5%). The mean age was 51.2 ± 12.8 years. Arterial hypertension was the first etiology of CKD (n = 13 or 39.4%). The prevalence of H. pylori in patients with CKD was 63.6% versus 37.9% in control patients (p-value = 0.015). The main endoscopic lesions were erosive gastropathy (n = 14 or 42.4%) and erythematous gastropathy (n = 7 or 21.2%). Patients with CKD were 5 times more likely to have H. pylori infection (OR = 5.69;CI 95% 0.14 - 0.82;p = 0.017). Factors associated with H. pylori infection were chronic kidney disease (aOR = 1.02;CI 95% 0.14 - 0.82;p = 0.017) and hemodialysis (aOR = 10;CI 95% 1.08 - 91.9;p = 0.042). Conclusion: The prevalence of H. pylori infection is higher in patients with CKD. Endoscopic lesions are inflammatory. Factors associated with H. pylori infection are chronic kidney disease and hemodialysis.展开更多
In this study, we investigated the preventive effects of a probiotic preparation (Miya BM) containing Clostridium butyricum spores against the side effects of Helicobacter pylori eradication. The rats were administere...In this study, we investigated the preventive effects of a probiotic preparation (Miya BM) containing Clostridium butyricum spores against the side effects of Helicobacter pylori eradication. The rats were administered omeprazole, amoxicillin and clarithromycin, which combination of drugs was a new triple-therapy proposed for the firstline therapy by the guideline of the treatment of H.pylori infection in Japan. Obligate anaerobes and Lactobacillus were decreased markedly, while aerobes were increased after the three drugs administration. Moreover, Clostridium difficile causative of antibioticassociated diarrhea was isolated from feces after the 7th. Administration of Miya BM decreased the number of C.difficile in the feces and increased the short chain fatty acids in the intestinal contents.展开更多
Isolation of Helicobacter pylori (HP) is the gold standard for the diagnosis of HP infection. However, the conventional isolation method is tedious, expensive and troublesome, and is not suitable for use in general pr...Isolation of Helicobacter pylori (HP) is the gold standard for the diagnosis of HP infection. However, the conventional isolation method is tedious, expensive and troublesome, and is not suitable for use in general practice. We have developed a simple, low-cost and effective method for the isolation of HP. Two biopsy specimens from each patient were inoculated on a HP selective blood plate, and placed in a specially devised plastic jar covered air-tightly with a lid. After filling with a mixture gas (CO2 10%, O2 5%, N2 85%),展开更多
The aim of this study is to evaluate the diagnostic value of ^14C-UBT for diagnosis of HP infection. The positive predictive values were above 100 bpm/mmol CO2. 202 of 256 patients with HP-positive infection were dete...The aim of this study is to evaluate the diagnostic value of ^14C-UBT for diagnosis of HP infection. The positive predictive values were above 100 bpm/mmol CO2. 202 of 256 patients with HP-positive infection were detected (sensitivity, 78%), what's more, breath test and RUT corresponded well. 256 patients diagnosed were investigated, aged 17-81 years (average 44.25 years). HP positive rate was 96.5% in 113 patients with peptic ulcer disease (PUD), 91.7% in 60 patients with chronic active gastritis, 73.3% in 15 patients with GERD,展开更多
文摘Introduction: Gastroduodenal lesions are common in chronic kidney disease (CKD). They are linked to various factors including Helicobacter pylori infection (H. pylori). Few data are available in Africa on H. pylori infection and chronic kidney disease. The aim of this study was to assess the impact of H. pylori infection and to describe the gastroduodenal lesions found in patients with chronic kidney disease. Patients and Methods: A cross-sectional study was conducted, February 1<sup>st</sup> to May 31<sup>st</sup>, 2021, at the Douala General Hospital in Cameroon. We included patients with CKD classified as stages 3 to 5 according KDIGO classification, on hemodialysis or not, who agreed to participate in the study. They were matched with a “control” population including patients with normal renal function according to sex and age (ratio 1:2). Patients on antibiotics and/or proton pump inhibitors were excluded. We collected data from CKD patients and from medical records for non-CKD group. Each patient underwent an upper digestive endoscopy and identification of H. pylori using a urease rapid test. Logistic regression was used to identify independent associations for a significance level set at p Results: We included 99 patients including 33 with CKD and 66 control patients. Among patients with CKD, the predominance was male (n = 18/33 or 54.5%). The mean age was 51.2 ± 12.8 years. Arterial hypertension was the first etiology of CKD (n = 13 or 39.4%). The prevalence of H. pylori in patients with CKD was 63.6% versus 37.9% in control patients (p-value = 0.015). The main endoscopic lesions were erosive gastropathy (n = 14 or 42.4%) and erythematous gastropathy (n = 7 or 21.2%). Patients with CKD were 5 times more likely to have H. pylori infection (OR = 5.69;CI 95% 0.14 - 0.82;p = 0.017). Factors associated with H. pylori infection were chronic kidney disease (aOR = 1.02;CI 95% 0.14 - 0.82;p = 0.017) and hemodialysis (aOR = 10;CI 95% 1.08 - 91.9;p = 0.042). Conclusion: The prevalence of H. pylori infection is higher in patients with CKD. Endoscopic lesions are inflammatory. Factors associated with H. pylori infection are chronic kidney disease and hemodialysis.
文摘In this study, we investigated the preventive effects of a probiotic preparation (Miya BM) containing Clostridium butyricum spores against the side effects of Helicobacter pylori eradication. The rats were administered omeprazole, amoxicillin and clarithromycin, which combination of drugs was a new triple-therapy proposed for the firstline therapy by the guideline of the treatment of H.pylori infection in Japan. Obligate anaerobes and Lactobacillus were decreased markedly, while aerobes were increased after the three drugs administration. Moreover, Clostridium difficile causative of antibioticassociated diarrhea was isolated from feces after the 7th. Administration of Miya BM decreased the number of C.difficile in the feces and increased the short chain fatty acids in the intestinal contents.
文摘Isolation of Helicobacter pylori (HP) is the gold standard for the diagnosis of HP infection. However, the conventional isolation method is tedious, expensive and troublesome, and is not suitable for use in general practice. We have developed a simple, low-cost and effective method for the isolation of HP. Two biopsy specimens from each patient were inoculated on a HP selective blood plate, and placed in a specially devised plastic jar covered air-tightly with a lid. After filling with a mixture gas (CO2 10%, O2 5%, N2 85%),
文摘The aim of this study is to evaluate the diagnostic value of ^14C-UBT for diagnosis of HP infection. The positive predictive values were above 100 bpm/mmol CO2. 202 of 256 patients with HP-positive infection were detected (sensitivity, 78%), what's more, breath test and RUT corresponded well. 256 patients diagnosed were investigated, aged 17-81 years (average 44.25 years). HP positive rate was 96.5% in 113 patients with peptic ulcer disease (PUD), 91.7% in 60 patients with chronic active gastritis, 73.3% in 15 patients with GERD,
