Tubeufiales,ord.nov.,integrating sexual and asexual generic names

Tubeufiaceae is based on the generic type Tubeufia,which is characterized by superficial,oval and bright ascomata,bitunicate asci,mostly long fusiform to filiform,transeptate ascospores and hyphomycetous asexual states with helicosporous conidia.Most species in this family are saprobic on terrestrial woody substrates and some are aquatic.Their distinct morphology as well as combined LSU,SSU and TEF1 sequence analysis show that Tubeufiaceae should be accommodated in a new order Tubeufiales,which is introduced in this paper.Phylogenetic analyses of combined LSU and ITS sequences were used to resolve genera and species within the family Tubeufiaceae.In this study,we examine and incorporate sexual and asexual states of genera in Tubeufiales to provide a modern treatment,based on single names.An epitype for Tubeufia javanica,the type species of Tubeufia,is designated and represents Tubeufia sensu stricto.The genera Acanthophiobolus,Acanthostigma,Boerlagiomyces,Chlamydotubeufia,Kamalomyces,Podonectria,Thaxteriella and Thaxteriellopsis are accepted,Acanthostigmina is reinstated,and the asexual genera Aquaphila,Helicoma,Helicomyces,Helicosporium and Tamhinispora are accepted in Tubeufiaceae.Three new genera Acanthohelicospora,Helicangiospora and Neoacanthostigma are introduced.The genus Bifrontia is added to the family based on morphological similarity.The incongruous morphological genera Acanthostigmella,Amphinectria,Chaetocrea,Chaetosphaerulina,Glaxoa,Malacaria,Melioliphila,Paranectriella,Puttemansia,Rebentischia and Uredinophila are excluded from Tubeufiaceae despite having characteristic ascomata with setae and multiseptate long spores.A key to genera accepted in Tubeufiaceae is provided.

A taxonomic reassessment of Tubeufiales based on multi-locus phylogeny and morphology

This study deals with an extensive taxonomic reevaluation focusing on phylogenetic relationships and morphological characterization of Tubeufiales,especially those helicosporous hyphomycetes which are difficult to identify.Based on evidence from DNA sequence data and morphology,we introduce 13 new genera in the family Tubeufiaceae,viz.Acanthotubeufia,Dematiohelicoma,Dematiohelicomyces,Dematiohelicosporum,Dematiotubeufia,Helicoarctatus,Heli-cohyalinum,Helicotruncatum,Neochlamydotubeufia,Neohelicoma,Pleurohelicosporium,Pseudohelicomyces and Pseu-dohelicoon;transfer Chaetosphaerulina from Dothideomycetes genera incertae sedis,and Artocarpomyces and Helicodochium from Ascomycetes genera incertae sedis into Tubeufiaceae;introduce 52 new species,viz.Berkleasmium fusiforme,B.longisporum,Chlamydotubeufia cylindrica,Dematiohelicosporum guttulatum,Helicoarctatus aquaticus,Helicodochium aquaticum,Helicohyalinum infundibulum,Helicoma aquaticum,H.brunneisporum,H.cocois,H.rufum,H.fusiforme,H.longisporum,H.multiseptatum,H.rubriappendiculatum,H.septoconstrictum,H.tectonae,Helicomyces hyalosporus,Helicosporium aquaticum,H.flavisporum,H.setiferum,H.vesicarium,H.viridiflavum,Neochlamydo-tubeufia fusiformis,Neohelicomyces hyalosporus,Neohelicosporium acrogenisporum,N.astrictum,N.ellipsoideum,N.irregulare,N.krabiense,N.laxisporum,N.ovoideum,Pleurohelicosporium parvisporum,Pseudohelicomyces aquaticus,P.hyalosporus,Tubeufia abundata,T.bambusicola,T.brevis,T.brunnea,T.chlamydospora,T.dictyospora,T.eccentrica,T.fangchengensis,T.hechiensis,T.inaequalis,T.krabiensis,T.rubra,T.sessilis,T.sympodihylospora,T.sympodilaxispora,T.taiwanensis and T.tratensis;provide 43 new combinations,viz.Acanthohelicospora guianensis,Acanthotubeufia filiforme,Berkleasmium aquatica,B.guangxiense,B.latisporum,B.thailandicum,Dematiohelicoma perelegans,D.pulchrum,Dematiohelicomyces helicosporus,Dematiotubeufia chiangraiensis,Helicohyalinum aquaticum,Helicoma eli-norae,H.gigasporum,H.hongkongense,H.linderi,H.nematosporum,H.pannosum,H.serpentinum,Helicomyces chiayiensis,Helicotruncatum palmigenum,Neochlamydotubeufia khunkornensis,Neohelicoma fagacearum,Neoheli-comyces pallidus,Neohelicosporium abuense,N.aurantiellum,N.griseum,N.morganii,N.myrtacearum,N.nizam-abadense,N.sympodiophorum,N.taiwanense,N.vesiculiferum,Pseudohelicomyces indicus,P.paludosus,P.talbotii,Pseudohelicoon gigantisporum,P.subglobosum,Tubeufia dentophora,T.geniculata,T.lilliputea,T.machaerinae,T.sympodiophora and T.xylophila;introduce 16 new records,viz.Dictyospora thailandica,Helicomyces colligatus,H.torquatus,Neohelicosporium guangxiense,N.hyalosporum,N.parvisporum,Thaxteriellopsis lignicola,Tubeufia aquatica,T.chiangmaiensis,T.cylindrothecia,T.filiformis,T.guangxiensis,T.laxispora,T.parvispora,T.roseohelicospora and T.ectonae.The taxonomy of Helicoma,Helicomyces and Helicosporium is revisited based on phylogenetic analyses and morphological evidence.Neorhamphoria is transferred to Bezerromycetaceae.Three species are excluded from the genus Chlamydotubeufia,twelve species from Helicoma,four species from Helicomyces,25 species from Helicosporium,six species from Neoacanthostigma and one species from Tubeufia.A multi-gene phylogenetic tree based on maximum likelihood and Bayesian analyses of ITS,LSU,RPB2 and TEF1a sequence data of species of Tubeufiales is provided.Detailed descriptions and illustrations are provided,as well as the morphological comparison with similar taxa are explored.The checklist of accepted Tubeufiales species and re-organised Tubeufiales species are provided.