Helicoverpa armigera single nucleocapsid nucleopolyhedrovirns open reading frame 101 （ha101） is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 ...Helicoverpa armigera single nucleocapsid nucleopolyhedrovirns open reading frame 101 （ha101） is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 were explored using BLASTP searching tool in the updated GenBank/EMBL and SWlSS-PROT databases. The results showed that the homologues of ha101 were present in all the completely sequenced lepidopteran nucleopolyhedrovirnses and granulovirnses, suggesting that ha101 might be a functional gene associated with their lepidopteran hosts. Sequence alignment of ha101 and its homologues revealed that 10 amino acids were completely conserved. RT-PCR analysis of ha101 manifested that the transcript of ha101 was first detected at 24 hpi and remained detectable at up to 122 hpi, suggesting that ha101 was transcribed during late stages of infection. Ha101 was expressed using Bac to Bac system in Tn5B-1-4 cells. The product of ha101 expressed in Tn5B-1-4 cells was approximately 29kDa, consistent with the predicted molecular weight, and the results were confirmed by western blot analysis. The subcellular localization indicated that ha101 was aggregated along nuclear envelope during the early stages of infection and spread out to the entire nucleus including virogenic stroma in late stages of infection, suggesting that ha101 may play a specific role in virion assembly process or virogenic stroma arrangement.展开更多
Baculoviruses are insect-specific viruses with a circular double-stranded DNA genome ranging in size from 80-180 kb （Lu et al., 2012）. Two distinct types of viri- ons have been identified during the infectious cycle...Baculoviruses are insect-specific viruses with a circular double-stranded DNA genome ranging in size from 80-180 kb （Lu et al., 2012）. Two distinct types of viri- ons have been identified during the infectious cycle of baculoviruses, namely budded virions （BVs） and occlu- sion-derived virions （ODVs）. BVs mediate infection from cell to cell, while ODVs initiate oral infection in the insect midgut （Braunagel and Summers, 2007）.展开更多
文摘Helicoverpa armigera single nucleocapsid nucleopolyhedrovirns open reading frame 101 （ha101） is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 were explored using BLASTP searching tool in the updated GenBank/EMBL and SWlSS-PROT databases. The results showed that the homologues of ha101 were present in all the completely sequenced lepidopteran nucleopolyhedrovirnses and granulovirnses, suggesting that ha101 might be a functional gene associated with their lepidopteran hosts. Sequence alignment of ha101 and its homologues revealed that 10 amino acids were completely conserved. RT-PCR analysis of ha101 manifested that the transcript of ha101 was first detected at 24 hpi and remained detectable at up to 122 hpi, suggesting that ha101 was transcribed during late stages of infection. Ha101 was expressed using Bac to Bac system in Tn5B-1-4 cells. The product of ha101 expressed in Tn5B-1-4 cells was approximately 29kDa, consistent with the predicted molecular weight, and the results were confirmed by western blot analysis. The subcellular localization indicated that ha101 was aggregated along nuclear envelope during the early stages of infection and spread out to the entire nucleus including virogenic stroma in late stages of infection, suggesting that ha101 may play a specific role in virion assembly process or virogenic stroma arrangement.
基金supported by the grants from the National Science Foundation of China(No.31570153,31130058,31321001 and 31400142)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB11030400)the Core Facility and Technical Support of Wuhan Institute of Virology for technical assistance
文摘Baculoviruses are insect-specific viruses with a circular double-stranded DNA genome ranging in size from 80-180 kb （Lu et al., 2012）. Two distinct types of viri- ons have been identified during the infectious cycle of baculoviruses, namely budded virions （BVs） and occlu- sion-derived virions （ODVs）. BVs mediate infection from cell to cell, while ODVs initiate oral infection in the insect midgut （Braunagel and Summers, 2007）.
