A novel set of five polymorphic di- or trinucleofide microsatellite loci suitable for population genetic study were developed from an enriched genomic library for the pest insect cotton bollworm, Helicoverpa armigera,...A novel set of five polymorphic di- or trinucleofide microsatellite loci suitable for population genetic study were developed from an enriched genomic library for the pest insect cotton bollworm, Helicoverpa armigera, and cross-amplifiability of these and other published loci was tested in a closely related species, the tobacco budworm, H.assulta. The expected heterozygosity at these loci ranges from 0.62 to 0.91 in the cotton bollworm. The observed allele numbers varies from 4 to 12 in the limited number of individuals tested. Although a large proportion of cloned microsatellite sequences are present in multi-copy in the cotton bollworm genome, the overwhelming majority of the finalized polymorphic diallelic loci are tri-nucleotide microsatellites - an unexpected outcome, which should facilitate subsequent genotyping analysis.展开更多
运用地统计学方法，分析了山东省滨州地区二代棉铃虫在1965-2004年间种群动态的时间序列结构特征和自相关性，根据决定系数尺。和Akaike’s Information Criteria（AIC）判别参数，筛选出最优拟合模型。结果表明：棉铃虫越冬蛹量、二代...运用地统计学方法，分析了山东省滨州地区二代棉铃虫在1965-2004年间种群动态的时间序列结构特征和自相关性，根据决定系数尺。和Akaike’s Information Criteria（AIC）判别参数，筛选出最优拟合模型。结果表明：棉铃虫越冬蛹量、二代棉铃虫百株累计卵量、发生程度的最优拟合模型均是球形模型，呈聚集分布；三者的变程分别为10．41、12和12．06年，高度一致；三者在时间序列上的自相关性分别是53．22％、52．62％和69．06％；三者的结构特征引起的种群变异介于25％～75％，为中等程度的空间自相关性，其相关强度顺序是百株累计卵量〈越冬蛹量〈发生程度，随机部分引起的空间变异则相反。展开更多
文摘A novel set of five polymorphic di- or trinucleofide microsatellite loci suitable for population genetic study were developed from an enriched genomic library for the pest insect cotton bollworm, Helicoverpa armigera, and cross-amplifiability of these and other published loci was tested in a closely related species, the tobacco budworm, H.assulta. The expected heterozygosity at these loci ranges from 0.62 to 0.91 in the cotton bollworm. The observed allele numbers varies from 4 to 12 in the limited number of individuals tested. Although a large proportion of cloned microsatellite sequences are present in multi-copy in the cotton bollworm genome, the overwhelming majority of the finalized polymorphic diallelic loci are tri-nucleotide microsatellites - an unexpected outcome, which should facilitate subsequent genotyping analysis.
文摘运用地统计学方法，分析了山东省滨州地区二代棉铃虫在1965-2004年间种群动态的时间序列结构特征和自相关性，根据决定系数尺。和Akaike’s Information Criteria（AIC）判别参数，筛选出最优拟合模型。结果表明：棉铃虫越冬蛹量、二代棉铃虫百株累计卵量、发生程度的最优拟合模型均是球形模型，呈聚集分布；三者的变程分别为10．41、12和12．06年，高度一致；三者在时间序列上的自相关性分别是53．22％、52．62％和69．06％；三者的结构特征引起的种群变异介于25％～75％，为中等程度的空间自相关性，其相关强度顺序是百株累计卵量〈越冬蛹量〈发生程度，随机部分引起的空间变异则相反。
