An Or83b-like receptor gene was cloned from antennae of Helicoverpa assulta(Guenée) by using reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE).Sequence analysis re...An Or83b-like receptor gene was cloned from antennae of Helicoverpa assulta(Guenée) by using reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE).Sequence analysis revealed that the transcript of the Or83b-like receptor gene from H.assulta consists of 1 946 nucleotides and the open reading frame(ORF) encodes a peptide of 473 amino acids with 7 putative transmembrane domains.Alignment analysis suggested that amino acid sequence of Or83b-like receptor from H.assulta shares high identity with other Or83b family receptors and this gene was hence named as HassOr83b.Tissues expression analysis showed that the HassOr83b transcript is clearly observed in the antennae,labial palps and proboscises,but not in bodies,wings and legs.The further development expression analysis suggested HassOr83b is also expressed in several preadult stages,including early-stage larvae,late-stage larvae and pupae,but not in embryos.Locked nucleic acid(LNA)-based in situ hybridization of antennal section indicated that HassOr83b is expressed in a very large number of antennal cells,which suggests that HassOr83b may play a special role in olfaction in H.assulta.展开更多
The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta (Guenée) by reverse transcription polymerase chain reaction (RT-PCR) and named as HassGαq. Sequencing analysis sh...The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta (Guenée) by reverse transcription polymerase chain reaction (RT-PCR) and named as HassGαq. Sequencing analysis showed that the full- length of HassGαq open reading frame (ORF) is 1 062 bp, 353 amino acid residues are encoded. The predicted molecular weights (MW) and isoelectric point (PI) are 41.5 kD and 5.15, respectively. HassGαq gene was then constructed into expression vector pGEX-4T-2 for over expression in prokaryotic cells. The SDS-PAGE and Western blot analysis showed that induced by Isopropyl-β-D-Thiogalactoside (IPTG), the GST-HassGαq fusion protein is expressed in Escherichia coli BL21, and its MW was found to be about 66 kD nearly equal to the predicted. In addition, RT-PCR analysis showed that the expressions of HassGαq are not tissue specific.展开更多
[Objective]The aim was to study the control effect of different sex pheromones and trapping lamps against main pests in tobacco. [Method]Control effect of sex pheromone of different matrix lures and Jiaduo trapping la...[Objective]The aim was to study the control effect of different sex pheromones and trapping lamps against main pests in tobacco. [Method]Control effect of sex pheromone of different matrix lures and Jiaduo trapping lamp against Spodoptern litura,Helicoverpa assulta and Helicoverpa armigera in tobacco in Tengchong of Yunnan were determined and compared,and the control cost was evaluated. [Result] The use of insect sex pheromones and insecticidal light traps had certain effect against S. litura and H. armiger. The trapping effect of sex pheromone traps was better than that of Jiaduo trapping lamp,and the PVC matrix lure had better performance than rubber matrix lure,which had strongest capturing capacity,continuous control effect and significant effort against S. litura. The traps with different settled densities and hanging heights also had different trapping effect against S. litura and H. armiger,and the hanging height of 100 - 150 cm from ground was the best; as the cost was considered,the cost of the area with low density of traps was the lowest,which was reduced by over 44% than conventional chemical control area. [Conclusion]It is safe and effective to use sex pheromone and insecticidal light traps to control S. litura and H. armigera,and it is a green environmental protection biological physical control technology,having extended application prospect in large area.展开更多
Eleven isolates of Beauveria spp., including B. bassiana(Bb062) newly isolated from Helicoverpa assulta, and other seven B. bassiana isolates and three B. brongniartii isolates that were originally isolated from diffe...Eleven isolates of Beauveria spp., including B. bassiana(Bb062) newly isolated from Helicoverpa assulta, and other seven B. bassiana isolates and three B. brongniartii isolates that were originally isolated from different geographic origins and various hosts, were tested against the 3^(rd) instar larvae of H. assulta. The protective enzyme activity in the 3^(rd) larvae of H. assulta infected by highly virulent isolate was also assayed. The results showed that the isolate Bb062 had the highest virulence to the 3^(rd) instar larvae among eleven isolates of Beauveria spp., with a corrected mortality reaching 91.07% within 10 d post treatment, and the LT_(50) was 4.67 d. After inoculated with three concentrations(1.0 ×10~6, 1.0 ×10~7 and 1.0×10~8 conidia/mL) of Bb062 conidial suspension, the accumulative mortality of H. assulta larvae increased with the increase of concentration and observation time, and the LC_(50) was 1.82×10~7 conidia/mL. Activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) in H. assulta larvae first increased rapidly then dropped sharply within 72 h. Bb062 showed high virulence to H. assulta and could inhibit activities of protective enzymes. Therefore, it will be a promising biocontrol agent against H. assulta.展开更多
The attraction of Helicoverpa armigera- and Helicoverpa assulta-induced and mechanical damage-in- duced tobacco volatiles to Campoletis chlorideae was investi-gated, and the induced volatiles were analyzed. In wind- t...The attraction of Helicoverpa armigera- and Helicoverpa assulta-induced and mechanical damage-in- duced tobacco volatiles to Campoletis chlorideae was investi-gated, and the induced volatiles were analyzed. In wind- tunnel, C. chlorideae was strongly attracted by herbivore- induced tobacco volatiles. Mechanically damaged tobacco leaves, whether treated with caterpillar regurgitant or water, were more attractive to the parasitoid than undamaged to-bacco leaves. GC-MS analysis revealed that only 4 com-pounds were released from undamaged tobacco leaves, whereas 13 compounds were commonly emitted from herbi-vore-infested and mechanically damaged tobacco leaves. Compound β-pinene was specifically induced by the infesta- tion of H. armigera, and (Z)-3-hexenal was only induced by the infestation of H. armigera and H. assulta, whereas hexyl acetate was only induced by mechanical damage. Tobacco leaves infested by H. armigera and H. assulta released larger amounts of volatiles than undamaged tobacco leaves did. Tobacco leaves treated with artificial damage plus caterpil- lars regurgitant or water emitted the same levels of volatiles, which were higher than that emitted by undamaged tobacco leaves. The emission amounts of single compounds were also different between differently treated plants. The differences were large between herbivore-induced and mechanical dam-age-induced compounds, and small between H. armigera- and H. assulta-induced compounds, and among compounds emitted from mechanically damaged plants treated with wa-ter or caterpillar regurgitant.展开更多
The dual-choices tests of behavioral test were used to study the gustatory behavioral responses to caffeine of Helicoverpa armigera larvae and H. assulta larvae. Electrophysiological responses were studied by electrop...The dual-choices tests of behavioral test were used to study the gustatory behavioral responses to caffeine of Helicoverpa armigera larvae and H. assulta larvae. Electrophysiological responses were studied by electrophysiological tip recording. Behavioral experiments showed that caffeine was a feeding deterrent for both larvae,but it showed a stronger feeding deterring effect on the oligophagous H. assulta. The electrophysiological tip-recording results showed that both H. armigera larvae and H. assulta larvae had one caffeine-sensitive feeding deterrent neuron at the medial sensilla,and the electrophysiological pulse response of H. assulta larvae was significantly stronger than that of H. armigera larvae. Therefore,caffeine had a stronger feeding deterring effect on the oligophagous H. assulta than the polyphagous H. armigera. The difference in behavioral effect was closely related to the sensitivity of to caffeine the feeding deterrent neurons at the medial sensilla.展开更多
基金supported by the Excellent Youth Foundation of Henan Province of China (074100510013)the Science and Technology Innovation Foundation of Henan Agricultural University of China (2007-CX-014)
文摘An Or83b-like receptor gene was cloned from antennae of Helicoverpa assulta(Guenée) by using reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends(RACE).Sequence analysis revealed that the transcript of the Or83b-like receptor gene from H.assulta consists of 1 946 nucleotides and the open reading frame(ORF) encodes a peptide of 473 amino acids with 7 putative transmembrane domains.Alignment analysis suggested that amino acid sequence of Or83b-like receptor from H.assulta shares high identity with other Or83b family receptors and this gene was hence named as HassOr83b.Tissues expression analysis showed that the HassOr83b transcript is clearly observed in the antennae,labial palps and proboscises,but not in bodies,wings and legs.The further development expression analysis suggested HassOr83b is also expressed in several preadult stages,including early-stage larvae,late-stage larvae and pupae,but not in embryos.Locked nucleic acid(LNA)-based in situ hybridization of antennal section indicated that HassOr83b is expressed in a very large number of antennal cells,which suggests that HassOr83b may play a special role in olfaction in H.assulta.
文摘The cDNA encoding the G protein αq subunit was isolated from the antennae of Helicoverpa assulta (Guenée) by reverse transcription polymerase chain reaction (RT-PCR) and named as HassGαq. Sequencing analysis showed that the full- length of HassGαq open reading frame (ORF) is 1 062 bp, 353 amino acid residues are encoded. The predicted molecular weights (MW) and isoelectric point (PI) are 41.5 kD and 5.15, respectively. HassGαq gene was then constructed into expression vector pGEX-4T-2 for over expression in prokaryotic cells. The SDS-PAGE and Western blot analysis showed that induced by Isopropyl-β-D-Thiogalactoside (IPTG), the GST-HassGαq fusion protein is expressed in Escherichia coli BL21, and its MW was found to be about 66 kD nearly equal to the predicted. In addition, RT-PCR analysis showed that the expressions of HassGαq are not tissue specific.
基金Supported by Key Project of China National Tobacco Company ( 110201202015)Project of Yunnan Tobacco Company ( 2012YN11)
文摘[Objective]The aim was to study the control effect of different sex pheromones and trapping lamps against main pests in tobacco. [Method]Control effect of sex pheromone of different matrix lures and Jiaduo trapping lamp against Spodoptern litura,Helicoverpa assulta and Helicoverpa armigera in tobacco in Tengchong of Yunnan were determined and compared,and the control cost was evaluated. [Result] The use of insect sex pheromones and insecticidal light traps had certain effect against S. litura and H. armiger. The trapping effect of sex pheromone traps was better than that of Jiaduo trapping lamp,and the PVC matrix lure had better performance than rubber matrix lure,which had strongest capturing capacity,continuous control effect and significant effort against S. litura. The traps with different settled densities and hanging heights also had different trapping effect against S. litura and H. armiger,and the hanging height of 100 - 150 cm from ground was the best; as the cost was considered,the cost of the area with low density of traps was the lowest,which was reduced by over 44% than conventional chemical control area. [Conclusion]It is safe and effective to use sex pheromone and insecticidal light traps to control S. litura and H. armigera,and it is a green environmental protection biological physical control technology,having extended application prospect in large area.
基金Supported by General Project of Shandong Branch of China National Tobacco Corporation(KJ20170413)Major Special Project of Guiyang Branch of Guizhou Tobacco Company(2016-06)+2 种基金Natural Science Foundation of Anhui Province(1908085MC70)Key Projects of Natural Science at Higher Institutions in Anhui Province(03087060)Key Projects of China National Tobacco Corp Sichuan Company(SCYC201703&SCYC201806&SCYC2018-2)
文摘Eleven isolates of Beauveria spp., including B. bassiana(Bb062) newly isolated from Helicoverpa assulta, and other seven B. bassiana isolates and three B. brongniartii isolates that were originally isolated from different geographic origins and various hosts, were tested against the 3^(rd) instar larvae of H. assulta. The protective enzyme activity in the 3^(rd) larvae of H. assulta infected by highly virulent isolate was also assayed. The results showed that the isolate Bb062 had the highest virulence to the 3^(rd) instar larvae among eleven isolates of Beauveria spp., with a corrected mortality reaching 91.07% within 10 d post treatment, and the LT_(50) was 4.67 d. After inoculated with three concentrations(1.0 ×10~6, 1.0 ×10~7 and 1.0×10~8 conidia/mL) of Bb062 conidial suspension, the accumulative mortality of H. assulta larvae increased with the increase of concentration and observation time, and the LC_(50) was 1.82×10~7 conidia/mL. Activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) in H. assulta larvae first increased rapidly then dropped sharply within 72 h. Bb062 showed high virulence to H. assulta and could inhibit activities of protective enzymes. Therefore, it will be a promising biocontrol agent against H. assulta.
基金supported by the National Natural Science Foundation of China(Grant No.30330100)the Chinese Academy of Sciences(Grant No.KSCX2-SW-105).
文摘The attraction of Helicoverpa armigera- and Helicoverpa assulta-induced and mechanical damage-in- duced tobacco volatiles to Campoletis chlorideae was investi-gated, and the induced volatiles were analyzed. In wind- tunnel, C. chlorideae was strongly attracted by herbivore- induced tobacco volatiles. Mechanically damaged tobacco leaves, whether treated with caterpillar regurgitant or water, were more attractive to the parasitoid than undamaged to-bacco leaves. GC-MS analysis revealed that only 4 com-pounds were released from undamaged tobacco leaves, whereas 13 compounds were commonly emitted from herbi-vore-infested and mechanically damaged tobacco leaves. Compound β-pinene was specifically induced by the infesta- tion of H. armigera, and (Z)-3-hexenal was only induced by the infestation of H. armigera and H. assulta, whereas hexyl acetate was only induced by mechanical damage. Tobacco leaves infested by H. armigera and H. assulta released larger amounts of volatiles than undamaged tobacco leaves did. Tobacco leaves treated with artificial damage plus caterpil- lars regurgitant or water emitted the same levels of volatiles, which were higher than that emitted by undamaged tobacco leaves. The emission amounts of single compounds were also different between differently treated plants. The differences were large between herbivore-induced and mechanical dam-age-induced compounds, and small between H. armigera- and H. assulta-induced compounds, and among compounds emitted from mechanically damaged plants treated with wa-ter or caterpillar regurgitant.
基金Supported by the National Natural Science Foundation of China(31201750)Hunan Provincial Natural Science Foundation of China(2016JJ4009)
文摘The dual-choices tests of behavioral test were used to study the gustatory behavioral responses to caffeine of Helicoverpa armigera larvae and H. assulta larvae. Electrophysiological responses were studied by electrophysiological tip recording. Behavioral experiments showed that caffeine was a feeding deterrent for both larvae,but it showed a stronger feeding deterring effect on the oligophagous H. assulta. The electrophysiological tip-recording results showed that both H. armigera larvae and H. assulta larvae had one caffeine-sensitive feeding deterrent neuron at the medial sensilla,and the electrophysiological pulse response of H. assulta larvae was significantly stronger than that of H. armigera larvae. Therefore,caffeine had a stronger feeding deterring effect on the oligophagous H. assulta than the polyphagous H. armigera. The difference in behavioral effect was closely related to the sensitivity of to caffeine the feeding deterrent neurons at the medial sensilla.
