Emerging evidence of the molecular landscape specific for hematogenous metastasis from gastric cancer

Gastric cancer(GC) is one of the most frequentlydiagnosed cancers in the world. Most GC patients are diagnosed when the cancer is in an advanced stage, and consequently, some develop metastatic lesions that generally cause cancer-related death. Metastasis establishment is affected by various conditions, such as tumor location, hemodynamics and organotropism. While digestive cancers may share a primary site, certain cases develop hematogenous metastasis with the absence of peritoneal metastasis, and vice versa. Numerous studies have revealed the clinicopathological risk factors for hematogenous metastasis from GC, such as vascular invasion, advanced age, differentiation, Borrmann type 1 or 2 and expansive growth. Recently, molecular mechanisms that contribute to metastatic site determination have been elucidated by advanced molecular biological techniques. Investigating the molecules that specifically participate in metastasis establishment in distinct secondary organs will lead to the development of novel biomarkers for patient stratification according to their metastatic risk and strategies for preventing and treating distinct metastases. We reviewed articles related to the molecular landscape of hematogenous metastasis from GC.

Hematogenous umbilical metastasis from colon cancer treated by palliative single-incision laparoscopic surgery

Sister Mary Joseph’s nodule(SMJN)is a rare umbilical nodule that develops secondary to metastatic cancer.Primary malignancies are located in the abdomen or pelvis.Patients with SMJN have a poor prognosis.An 83-year-old woman presented to our hospital with a1-month history of a rapidly enlarging umbilical mass.Endoscopic findings revealed advanced transverse colon cancer.computer tomography and fluorodeoxyglucose-positron emission tomography revealed tumors of the transverse colon,umbilicus,right inguinal lymph nodes,and left lung.The feeding arteries and drainage veins for the SMJN were the inferior epigastric vessels.Imaging findings of the left lung tumor allowed for identification of the primary lung cancer,and a diagnosis of advanced transverse colon cancer with SMJN and primary lung cancer was made.The patient underwent local resection of the SMNJ and subsequent single-site laparoscopic surgery involving right hemicolectomy and paracolic lymph node dissection.Intra-abdominal dissemination to the mesocolon was confirmed during surgery.Histopathologically,the transverse colon cancer was confirmed to be moderately differentiated tubular adenocarcinoma.We suspect that SMJN may occur via a hematogenous pathway.Although chemotherapy for colon cancer and thoracoscopic surgery for the primary lung cancer were scheduled,the patient and her family desired home hospice.Seven months after surgery,she died of rapidly growing lung cancer.

Intussusception due to hematogenous metastasis of hepatocellular carcinoma to the small intestine:A case report

BACKGROUND The commonest sites of extrahepatic metastases from hepatocellular carcinoma(HCC)are the lungs,bones,adrenal glands,and regional lymph nodes.Hematogenous metastasis to the gastrointestinal(GI)tract is a rare condition in patients with HCC,and the prognosis is usually poor.We report,herein,an extremely rare case of a patient with intussusception due to hematogenous metastasis of HCC to the ileum and his long-term survival with multidisciplinary therapy.CASE SUMMARY The patient was a 71-year-old man with a history of chronic hepatitis B,who had undergone three surgeries for HCC.He was treated with sorafenib for peritoneal metastases of HCC.He was admitted to our hospital with chief complaints of abdominal pain and vomiting.Abdominal contrast-enhanced computed tomography imaging revealed a small intestinal tumor,presenting with intussusception and small bowel obstruction.Conservative treatment was started,but due to repeated exacerbation of symptoms,surgery was planned on the 28th d of hospitalization.Partial ileal resection without reducing the intussusception and end-to-end anastomosis was performed.On histological examination,tumor cells were not observed on the serosal surface,but intravascular invasion of tumor cells was seen.Immunohistochemistry was positive for immunohistochemical markers,and a diagnosis of hematogenous metastasis of HCC to the ileum was made.He remains alive 82 mo after the first surgery.CONCLUSION Prognosis of HCC patients with GI tract metastasis is usually poor,but in some cases,multidisciplinary therapy may prolong survival.

Reciprocal hematogenous osteomyelitis of the femurs caused by Anaerococcus prevotii:A case report

BACKGROUND Haematogenous osteomyelitis is an extremely rare disease occurring in adults,especially in developed countries.It is clearly a systemic infection,because bacteraemia spreads over proximal and distal long bones or paravertebral plexuses,resulting in acute or chronic bone infection and destruction.CASE SUMMARY A 46-year-old Caucasian male was complaining of a left thigh pain.It is known from the anamnesis that the patient developed severe pneumonia three months ago before the onset of these symptoms.The patient was diagnosed with haematogenous osteomyelitis,which developed a turbulent course and required complex combination therapy.The primary pathogen is thought to be Anaerococcus prevotii,which caused pneumonia before the onset of signs of osteomyelitis.Unfortunately,due to the complexity of identifying anaerobes and contributing nosocomial infections,the primary pathogen was not extracted immediately.After the manifestation of this disease,pathological fractures occurred in both femurs,as well as purulent processes in the lungs and molars accompanied.The patient received broad-spectrum antibiotic therapy and countless amounts of orthopaedic and reconstructive surgeries,but no positive effect was observed.The patient underwent osteosynthesis using an Ilizarov’s external fixation apparatus,re-fixations,external AO,debridements,intramedullary osteosynthesis with a silver-coated intramedullary nail,abscessotomies.The right femur healed completely after the pathological fracture and osteomyelitis did not recur.Left femur could not be saved due to non-healing,knee contracture and bone destruction.After almost three years of struggle,it was decided to amputate the left limb,after which the signs of osteomyelitis no longer appeared.CONCLUSION To sum it all up,complicated or chronic osteomyelitis requires surgery to remove the infected tissue and bone.Osteomyelitis surgery prevents the infection from spreading further or getting even worse up to such condition that amputation is the only option left.

Hematogenous Osteomyelitis by Acinetobacter Baumannii: Case Report and Literature Review

Skeletal infection with Acinetobacter baumanii is a rare condition and found mainly among soldiers injured in war. Multidrug resistant (MDR) Acinetobacter baumanii (A. baumanii) osteomyelitis is difficult to treat and requires long course of intravenous antibiotics. Most of reported cases in the literature are the consequences of direct inoculation of the pathogen. Here in, we report the first case of A. Baumannii osteomyelitis disseminated through hematogenous route and the therapeutic approach for this rare infection. Clinical Presentation: A 46 year old African-American male patient with human immunodeficiency virus (HIV) and end- stage renal disease on hemodialysis, who developed persistent MDR A. baumanii bacteremia in the hospital, thought to be secondary to the hemodialysis catheter, necessitating replacement of the catheter. Three months after his discharge to a skilled nursing facility (SNF), he developed left leg swelling without noticeable pain or fever. MRI revealed findings consistent with chronic osteomyelitis of left tibia and intra operative bone culture grew MDR A. baumannii. The patient had good outcome after three surgical debridements and prolonged period (7 Months) of dual antimicrobial therapy. Discussion: While in most documented cases of A. baumannii osteomyelitis, entry appears to require direct inoculation;our case suggests that this pathogen can seed into bone hematogenously in the setting of immunosuppression, persistent bacteremia and possibly in the presence of underlying bone infarcts. Clinicians need to be aware of this rare possible consequence of A. baumannii bacteremia. In conclusion, combination of multiple surgical debridements and dual antimicrobial therapy for a long period may result in a good outcome.

Misdiagnosis of a multi-organ involvement hematogenous disseminated tuberculosis as metastasis:a case report and literature review

Background:Tuberculosis(TB)is a great mimicker and diagnostic chameleon,and prone to be diagnosed as malignancy.Even though many reports have described the differences between pulmonary TB and lung cancer,the atypical systemic hematogenous disseminated TB(HDTB)is very rare and more confusing in clinical practice.Case presentation:A 73-year-old man,HIV-negative,was hospitalized to the local county hospital because of chest pain,low-grade fever,asthenia,anorexia and weight loss for the pasting two months.The CT findings of the two lungs showed multiple round or round-like nodules of different sizes,with clear boundaries and partial fusion.The level of serum CA19–9 was significantly higher than normal,and progressively increased.There were multiple enlarged lymph nodes in the neck,mediastinum,abdominal cavity and pelvic cavity.The symptoms were diagnosed as hematogenous spread of gastrointestinal tumor in the local county hospital.However,when transferred to our provincial hospital,through comprehensive dynamic analysis,this patient was diagnosed as atypical systemic HDTB,no cancer at all.Through routine anti-TB therapy for one year,the patient was recovered very well at the follow-up of half year after withdrawal.Conclusions:In the past,most TB misdiagnosis cases involved in single organ and were finally confirmed through invasive examination.This case enriched clinical experiences in the diagnosis of atypical HDTB.We encouraged clinicians to establish a dynamic thinking for diagnosis and treatment and emphasized the value of biopsy and 18FFDG-PET in distinguishing TB and cancer.

Treatment analysis of acute fatty liver of pregnancy complicated with hematogenous infection of CRKP

Acute fatty liver of pregnancy(AFLP)is an idiopathic disease with acute and critical onset.Although the incidence of AFLP is relatively low,its mortality remains high.When AFLP is complicated with multi-organ dysfunction syndrome(MODS)and severe infection,especially hematogenous infection caused by multidrug-resistant pathogens,treatment becomes extremely difficult with an even lower survival rate.In the present work,we reported a 31-year-old primipara woman who developed AFLP complicated with MODS and severe infection at 37+5 weeks of gestation and received treatment in our hospital.The result of fast blood culture was positive for carbapenem-resistant Klebsiella pneumonia(CRKP).Based on this finding in combination with the results of drug sensitivity test,the quadruple regime using scheme imipenem cilastatin sodium,tigecycline,polymyxin and fosfomycin was implemented,and the patient was successfully cured.Successful treatment of pregnant women with AFLP complicated with MODS and/or hematogenous infection of CRKP can be achieved by using individualized combined antibiotic therapies on the basis of fast blood culture and combined drug sensitivity test.In this case,the patient was in a critical situation.In the treatment process,clinicians,clinical pharmacists and microbiologists should cooperate with each other and discuss the treatment plan together according to the pathophysiological characteristics of the patient,which was one of the key factors for successful treatment of the patient.

Tumor cell invasion from the marginal sinus into extranodal veins during early-stage lymph node metastasis can be a starting point for hematogenous metastasis

Aim:To investigate whether tumor cells in a lymph node(LN)can invade from the marginal sinus into extranodal veins via vessel branches that communicate with intranodal veins and whether this can be a starting point for hematogenous metastasis at the early stage of LN metastasis.Methods:Vascular and lymphatic networks of LNs in MXH10/Mo-lpr/lpr mice were investigated using three-dimensional micro-computed tomography and histological methods.Flow in the blood vessel networks of LNs was investigated by fluorescence microscopy.Tumor cells were injected into the subiliac LNs of MXH10/Mo-lpr/lpr mice to induce metastasis to the proper axillary LNs.Tumor development in the proper axillary LN was detected using an in vivo bioluminescence imaging system.A two-dimensional image of the proper axillary LN microvasculature was reconstructed using a contrast-enhanced high-frequency ultrasound system.Results:Extranodal veins communicated with intranodal veins via branches that penetrated the capsule,and blood flowed from intranodal veins to extranodal veins.Tumor cells that had metastasized to the marginal sinus invaded these communicating veins to develop hematogenous metastases.Conclusion:Metastatic LNs that would be considered by clinical imaging to be stage N0 can be a starting point for hematogenous metastasis.The study findings highlight the need for the development of novel techniques for the diagnosis and treatment of early-stage LN metastasis,i.e.,when standard diagnostic imaging might incorrectly classify the LN as stage N0.

Alterations of blood serum parameters in patients with chronic hematogenous osteomyelitis

Objective:To examine metabolic disorders of major components of organic basis of bone tissue in patients with chronic hematogenous osteomyelitis and response to surgical treatment.Methods:The cubital vein puncture was conducted to take blood for analysis in patients with chronic hematogenous osteomyelitis.The activity of collagenase and hyaluronidase,elastin,elastase and total content of glycosaminoglycans were measured in blood serum.Results:The study revealed an enhancement of catabolic phase of metabolism of the main components in bone organic matrix during the relapse of inflammation.It was evidenced by indicators reflecting the synthetic and catabolic phases of the main components of the connective tissue collagen and glycosaminoglycans.The effective therapeutic treatments led to the reduction and normalization of studied compounds.Conclusions:The initial development of hematogenous osteomyelitis happens in a background of metabolic disorders of the main components of organic matrix of bone tissue,and normalizes upon effective therapy.

Role of ^(18)F-FDG PET/CT in detection of hematogenous metastases of advanced differentiated thyroid carcinoma: a systematic review and meta-analysis

The aim of this study was to collect the evidence on the performance of 2-deoxy-2-[^(18)F]fluoro-D-glucose(^(18)F-FDG)Positron Emission Tomography/Computed Tomography(PET/CT)in terms of detection rate(DR)and diagnostic accuracy in identifying hematogenous metastases in patients with differentiated thyroid cancer and compare its performance with that of other imaging techniques.A comprehensive PubMed/MEDLINE database research was carried out to retrieve studies documenting the performance of^(18)F-FDG PET/CT in depicting hematogenous metastases in patients with differentiated thyroid cancer.Statistical analysis was performed on a per-patient and per-lesion basis.The literature search yielded 15 articles to be included in the systematic review.^(18)F-FDG PET/CT showed a pooled DR of 85.08%on a per-patient analysis and 89.70%on a per-lesion analysis.For bone lesions,a high DR(81.78%)was found for^(18)F-FDG PET/CT.For the sub-group analysis of lung lesions,pooled DR was 92.77%for^(18)F-FDG PET/CT,95.02%for CT,and 64.93%for magnetic resonance imaging(MRI).On a per-patient analysis,^(18)F-FDG PET/CT demonstrated a pooled sensitivity(SS)of 87.3%[95%confidence interval(CI):77.3%-94%]and a pooled specificity(SP)of 95.6%(95%CI:87.6-99.1).On a per-lesion analysis(328 metastases),PET/CT showed a pooled SS and SP of 86.3%(95%CI:73.5%-93.5%)and 93.4%(95%CI:71.7%-98.8%)in the detection of hematogenous metastases.The presented systematic review and meta-analysis favor the use of^(18)F-FDG PET/CT in the detection of hematogenous metastases in patients with differentiated thyroid cancer.The limited literature warrants further studies to confirm our findings.

Waist subcutaneous soft tissue metastasis of rectal mucinous adenocarcinoma: A case report

BACKGROUND Rectal mucinous adenocarcinoma(MAC)is a rare pathological type of rectal can-cer with unique pathological features and a poor prognosis.It is difficult to diag-nose and treat early because of the lack of specific manifestations in some aspects of the disease.The common metastatic organs of rectal cancer are the liver and lung;however,rectal carcinoma with metastasis to subcutaneous soft tissue is a rare finding.CASE SUMMARY In this report,the clinical data,diagnosis and treatment process,and postope-rative pathological features of a patient with left waist subcutaneous soft tissue masses were retrospectively analyzed.The patient underwent surgical treatment after admission and recovered well after surgery.The final pathological diagnosis was rectal MAC with left waist subcutaneous soft tissue metastasis.CONCLUSION Subcutaneous soft tissue metastasis of rectal MAC is rare,and it can suggest that the tumor is disseminated,and it can appear even earlier than the primary ma-lignant tumor,which is occult and leads to a missed diagnosis and misdiagnosis clinically.When a subcutaneous soft tissue mass of unknown origin appears in a patient with rectal cancer,a ma-lignant tumor should be considered.

β-毒素促进金黄色葡萄球菌破坏血脑屏障的实验研究

目的 探讨β-毒素(Hlb)在金黄色葡萄球菌通过血脑屏障(blood-brain barrier, BBB)中的作用。方法 通过血琼脂平板划线筛选金葡菌MW2的Hlb高产菌株,验证其对hCMEC/D3模拟BBB细胞模型通透性的影响。采用生物信息学分析筛选hlb基因完整的金葡菌菌株,利用同源重组的方法构建hlb敲除株,血琼脂平板法检测溶血活性改变。利用BBB细胞模型比较不同菌株对其通透性的影响,再将野生株与hlb敲除株经尾静脉注射构建小鼠血源性脑膜炎/脑微脓肿模型并比较感染72 h脑组织中菌载量。结果 金葡菌MW2的Hlb高产菌株对BBB细胞模型破坏导致BBB细胞模型通透性增加的能力较野生株明显增强。NCTC8325-4、COL和RN4220菌株hlb基因无噬菌体插入。成功构建NCTC8325-4Δhlb株,血平板证实敲除株的β-溶血缺失。NCTC8325-4Δhlb株对BBB模型细胞破坏导致BBB细胞模型通透性增加的作用较野生株下降,在小鼠血源性脑膜炎/脑微脓肿模型脑组织中菌载量也较野生株明显降低。结论 β-毒素具有促进金葡菌破坏BBB的作用。

牙龈卟啉单胞菌在非酒精性脂肪肝病进展中的作用

以牙龈卟啉单胞菌为代表的牙周致病菌不仅危害口腔健康,还与非酒精性脂肪肝病等口腔外疾病有着重要联系。牙龈卟啉单胞菌及其产物可通过血行途径和肠道途径等多种途径促进非酒精性脂肪肝病的发展,本文就相关作用途径及可能机制作一综述。

APACHEⅡ和SOFA评分与血源性鲍曼不动杆菌感染患者预后的相关性研究

目的:探讨急性生理学与慢性健康状况评分系统Ⅱ(APACHEⅡ)和序贯器官衰竭(SOFA)评分与血源性鲍曼不动杆菌感染患者28 d死亡的相关性。方法:回顾性分析2018年5月-2023年5月天津医科大学总医院收治的91例血源性鲍曼不动杆菌感染患者临床资料,通过Logistic回归分析影响血源性鲍曼不动杆菌感染患者28 d死亡的独立危险因素。应用受试者操作特征(ROC)曲线,分析APACHEⅡ和SOFA评分对此类患者预后的预测价值。结果:单因素分析显示,影响血源性鲍曼不动杆菌感染患者28 d预后的危险因素包括年龄、入住ICU、住院天数、肺炎、手术、深静脉置管、纤维支气管镜、肾功能不全、APACHEⅡ评分和SOFA评分等指标。多因素Logistic分析显示,APACHEⅡ和SOFA评分是血源性鲍曼不动杆菌感染患者28 d预后的独立危险因素,手术是鲍曼不动杆菌感染的保护因素。ROC曲线分析结果显示,死亡组首日的APACHEⅡ及SOFA评分均高于存活组,差异均有统计学意义(P<0.05)。结论:血流感染当天的APACHEⅡ及SOFA评分对评价血源性鲍曼不动杆菌感染患者28 d预后具有重要价值。

血行播散性肺结核合并颅内结核的临床特点

目的分析血行播散性肺结核合并颅内结核的临床特点,以提高临床医生的诊断意识。方法选取341例血行播散性肺结核患者,其中83例合并颅内结核。收集并分析患者的性别、年龄、既往史、临床表现;痰抗酸杆菌涂片、痰结核分枝杆菌培养、痰结核分枝杆菌/利福平耐药实时荧光定量核酸扩增技术(X-pert);颅内压,脑脊液常规、生化,脑脊液抗酸杆菌涂片、结核分枝杆菌培养、X-pert;血常规、丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、白蛋白、C反应蛋白、红细胞沉降率、结核抗体、结核感染T细胞检测(T-SPOT);胸部CT平扫、头颅CT平扫及增强扫描、头颅MRI平扫及增强扫描检查结果。结果83例合并颅内结核患者的症状以发热(74例)、头痛(53例)为主,11例合并糖尿病,9例使用激素/免疫抑制剂,4例合并妊娠/分娩/流产,6例合并腹腔结核,6例合并骨关节结核,2例生殖系统结核,1例淋巴结结核,1例泌尿系统结核,1例口腔黏膜结核。49例血钠降低(<135 mmol/L),平均131.0(126.2,135.8)mmol/L。44例患者的脑脊液蛋白质≥1 g/L,37例患者的脑脊液葡萄糖水平<2.2 mmol/L,25例患者的脑脊液腺苷脱氨酶(ADA)≥6 U/L。83例患者均行胸CT检查,64例显示粟粒性结节,33例表现为单纯急性血行播散性肺结核,21例表现为单纯亚急性和慢性血行播散性肺结核,29例合并继发性肺结核。34例患者行头颅CT检查,其中梗死灶10例,结核瘤4例,脑积水1例。51例行头颅MRI检查,其中脑基底部脑膜强化10例,脑梗死灶7例,结核瘤4例。结论充分评估血行播散性肺结核患者的年龄、症状、头胸部影像学、脑脊液、血钠等有助于诊断颅内结核。

创客教育在本科护生手术室血源性职业暴露教学中的应用研究

目的探索创客教育在本科护生手术室血源性职业暴露教学中的应用效果。方法2023年7-8月,将在我院手术室实习的本科护生81人随机分为对照组40人与观察组41人。对照组采用手术室常规教学;观察组采用创客教学。比较2组护生出科理论及操作考核成绩、实习前后创造性思维测验得分、对教学方法满意度及在手术室实习期间血源性职业暴露发生率和血源性职业暴露后处理正确率。结果观察组出科操作考核成绩及实习后创造性思维测验得分显著高于对照组,差异有统计学意义(P<0.05),观察组护生对教学方法的满意度较高(P<0.05)。结论创客教育可以有效地提高护生对血源性职业暴露知识的掌握,提高护生对教学的满意度,减少血源性职业暴露的发生。

牛至精油对新生犊牛生长发育和血液免疫的影响

选择40头新生犊牛,按出生日期随机分为试验组和对照组,每组各20头,试验组犊牛精料按40g·t^(-1)的比例添加牛至(Origanum vulgare)精油,旨在研究天然添加剂牛至精油对新生犊牛生长发育和血液免疫的影响。从犊牛出生到70日龄,跟踪研究了两组犊牛生长性能和血液免疫指标。结果表明,随着犊牛生长、发育时间的增加,试验组犊牛体重、体高、体长、胸围、腹围、管围、血清总蛋白、免疫球蛋白G、免疫球蛋白A、免疫球蛋白M以及平均采食量均极显著高于对照组(P<0.01),且试验组犊牛腹泻次数极显著降低(P<0.01)。由此可见,精料中添加牛至精油有促进犊牛生长发育、增强犊牛免疫力、增加犊牛采食量和降低犊牛腹泻发病率的作用。

血小板介导肿瘤血行转移的作用及其机制研究进展

肿瘤转移是恶性肿瘤最为重要的生物学特性之一,同时也是影响预后及导致治疗失败的主要因素。临床研究显示,恶性肿瘤患者的血小板数目较正常人及良性肿瘤患者明显升高,表明血小板可能参与了肿瘤的发生发展过程。进一步研究发现,血小板在肿瘤血行转移的过程中能够与肿瘤细胞相互作用形成瘤栓,逃避免疫监视,进而促进了肿瘤的转移。近年来,临床上已经开展了针对血小板治疗肿瘤转移的研究,同时,血小板促进肿瘤转移的相关作用机制也逐步地被揭示。该文对近年来血小板介导肿瘤血行转移的作用及其作用机制研究进展进行综述,探讨临床针对血小板治疗肿瘤转移的策略,为后续的科学研究及指导临床提供参考。

抗原激活系统血液免疫反应实验研究

目的:用系统免疫学研究体系探讨在系统血液免疫反应中不同抗原物质激活红细胞调控白细胞免疫反应的实验观察。方法:将卡介苗(1 mg/ml)、瘤苗(S180 5×106ml-1)或酵母菌(5×108ml-1)0.2 ml,以生理盐水(代替抗原物质)0.2 ml为各自对照组加入枸橼酸抗凝全血细胞或白细胞0.2 ml和血浆(或生理盐水0.3 ml)中,37℃水浴1小时,用免疫酶联法测定IL-8和IL-6,用流式细胞仪测定Fy6和CD35分子平均荧光强度。结果:各种抗原物质加入血细胞和血浆组其IL-8激活率为(2.71±0.37)显著高于血细胞和生理盐水组(-0.33±0.05)(P<0.01)。前组IL-6激活率(1.01±0.37)也高于后组,其IL-6激活值为(-0.25±1.06)。各种抗原加入全血细胞和血浆组的IL-8激活率为(2.71±0.37)显著高于各种抗原加入白细胞和血浆组IL-8激活率(0±0.20)(P<0.01)。在各种抗原加入血液组红细胞Fy6平均荧光强度表达水平(28.85和25.35)低于生理盐水加入血液组红细胞Fy6平均荧光强度表达水平(45.80),各种抗原加入全血细胞和血浆组粒细胞CD35平均荧光强度表达量(860.4±115.63)明显高于各种抗原加入白细胞血浆组粒细胞CD35平均荧光强度表达量(565.07±96.35)(P<0.01)。在无血浆组,红细胞对白细胞分泌的IL-8和IL-6有吸附作用。结论:在本实验体系中,各种抗原可激活红细胞调控系统血液免疫反应中的各种白细胞被活化所分泌的IL-8和IL-6含量。在体外抗原可快速激活系统血液免疫反应。红细胞调控各种白细胞释放IL-8和IL-6的机制是复杂的,如红细胞对IL-8和IL-6激活率与血浆和CD35分子量表达密切相关,红细胞IL-8和IL-6吸附率与Fy6和gp130表达密切相关。红细胞与血浆在系统血液免疫反应调控中扮演着重要的角色。

血源性颅面转移瘤动物模型及磁共振观察

目的 介绍建立血源性颅面转移瘤动物模型的方法 ,分析成瘤率的影响因素。方法 43只新西兰大白兔从颈总动脉接种VX2约 10 8/ml瘤细胞。术后 1周开始在不同时间行MRI检查 (平扫和增强扫描 )。病理取材HE染色光镜下观察。结果 面部及眶内球后成瘤率分别为 81%及 6 7%。MRI表现为T1WI略高信号 ,T2 WI等或高信号。T1WIGd TDPA扫描肿瘤有增强效应。结论 上述方法制成的颅面部动物模型可为颅面部医学影像学及其它学科的研究提供可靠的动物模型。