通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200...通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200 mW/cm2的溴钨灯光照射30 m in杀灭了99.9999%的单增李斯特菌,并导致了其蛋白质降解和基因组DNA片段断裂。血啉甲醚对单增李斯特菌的光动力灭活作用非常显著,其灭活机理可能是通过对蛋白质降解和基因组DNA损伤实现的。展开更多
The main objective of this study is to evaluate the antibacterial effect of antibacterial pho-todynamic therapy(aPDT)on Streptococcus mutans(S.mutans)biofilm model in vitro.The selection of photosensitizers is the key...The main objective of this study is to evaluate the antibacterial effect of antibacterial pho-todynamic therapy(aPDT)on Streptococcus mutans(S.mutans)biofilm model in vitro.The selection of photosensitizers is the key step for the efficacy of photodynamic therapy(PDT).However,no studies have been conducted in the oral field to compare the functional char-acteristics and application effects of PDT mediated by various photosensitizers.In this re-search,the antibacterial effect of Methylene blue(MB)/650 nm laser and Hematoporphyrin monomethyl ether(HMME)/532 nm laser on S.mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT.The yield of lactic acid was analyzed by Colony forming unit(CFU)and spectrophotometry,and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy(CLSM)to evaluate the bactericidal effect on each group.Based on the results of CFU,the bacterial colonies formed by 30.4J/cm^(2)532nm MB-aPDT group and 30.4J/cm^(2)532nm HMME-aPDT group were significantly less than those in other groups,and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group.Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group.The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4 J/cm^(2) aPDT.Therefore,aPDT suitable for energy measurement can kill S.mutans plaque biofilm,and MB-aPDT is better than HMME-aPDT.展开更多
文摘通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200 mW/cm2的溴钨灯光照射30 m in杀灭了99.9999%的单增李斯特菌,并导致了其蛋白质降解和基因组DNA片段断裂。血啉甲醚对单增李斯特菌的光动力灭活作用非常显著,其灭活机理可能是通过对蛋白质降解和基因组DNA损伤实现的。
基金supported by the Construction Plan of the Tianjin Characteristic Subject Group,Oral Medical Engineering
文摘The main objective of this study is to evaluate the antibacterial effect of antibacterial pho-todynamic therapy(aPDT)on Streptococcus mutans(S.mutans)biofilm model in vitro.The selection of photosensitizers is the key step for the efficacy of photodynamic therapy(PDT).However,no studies have been conducted in the oral field to compare the functional char-acteristics and application effects of PDT mediated by various photosensitizers.In this re-search,the antibacterial effect of Methylene blue(MB)/650 nm laser and Hematoporphyrin monomethyl ether(HMME)/532 nm laser on S.mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT.The yield of lactic acid was analyzed by Colony forming unit(CFU)and spectrophotometry,and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy(CLSM)to evaluate the bactericidal effect on each group.Based on the results of CFU,the bacterial colonies formed by 30.4J/cm^(2)532nm MB-aPDT group and 30.4J/cm^(2)532nm HMME-aPDT group were significantly less than those in other groups,and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group.Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group.The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4 J/cm^(2) aPDT.Therefore,aPDT suitable for energy measurement can kill S.mutans plaque biofilm,and MB-aPDT is better than HMME-aPDT.
