The coexistence of hepatocellular carcinoma(HCC) and non-Hodgkin's lymphoma(NHL) in the liver is rare. Reports show that these patients have cirrhotic livers or hepatitis virus infections before they develop HCC a...The coexistence of hepatocellular carcinoma(HCC) and non-Hodgkin's lymphoma(NHL) in the liver is rare. Reports show that these patients have cirrhotic livers or hepatitis virus infections before they develop HCC and NHL. We present a patient with hepatitis B virus infection who was transferred to our hospital with a newly detected liver mass; abdominal computed tomography examination showed one hypodense mass of 7 cm in diameter and multiple mesenteric and mediastinal lymph nodes. A liver tumor biopsy showed a hepatoma, and the pathologic findings from an inguinal lymph node excision showed mantle cell lymphoma. An immunohistochemical stain confirmed that the atypical lymphoid cells within the HCC were positive for the CD20, CD5 and cyclin D1 antigens. Taking these findings into account, the hepatic tumor was determined to be a HCC infiltrated by mantle cell lymphoma.展开更多
目的用48 h 快速培养获得的成熟树突细胞与肝癌细胞系 HCCLM3构建融合细胞疫苗。方法用 CD14正选磁珠从外周血中分离出CD14^+细胞,加入含有 GM-CSF 和 IL4的树突细胞完全培养基培养24 h,再加入肿瘤坏死因子(TNF)-α、IL-1β、IL-6和 PG...目的用48 h 快速培养获得的成熟树突细胞与肝癌细胞系 HCCLM3构建融合细胞疫苗。方法用 CD14正选磁珠从外周血中分离出CD14^+细胞,加入含有 GM-CSF 和 IL4的树突细胞完全培养基培养24 h,再加入肿瘤坏死因子(TNF)-α、IL-1β、IL-6和 PGE2,继续培养24 h 获得树突细胞并检测免疫分子 CD80、CD86、CD83和 HLA-DR 的表达。用50%聚乙二醇+10%二甲基亚砜融合 HCCLM3与所获树突细胞构建融合细胞并检测其免疫分子的表达。自体 T 细胞增殖实验按照刺激细胞不同分为融合细胞组(RH)、树突细胞组(DC)、HCCLM3组(H)及树突细胞与 HC-CLM3混合组(HH)。结果48 h 培养获得成熟树突细胞的 CD80、CD86、CD83和 HLA-DR 表达率可达94.43%、99.71%、62.78%和99.34%,它与 HCCLM3构建的融合细胞同样表达此类免疫分子,并且对自体 T 细胞增殖的刺激作用更强(P<0.05)。结论用48 h 快速培养获得的树突细胞可以成功构建能有效刺激自体 T 细胞增殖反应的融合细胞,该方法优点显著。展开更多
文摘The coexistence of hepatocellular carcinoma(HCC) and non-Hodgkin's lymphoma(NHL) in the liver is rare. Reports show that these patients have cirrhotic livers or hepatitis virus infections before they develop HCC and NHL. We present a patient with hepatitis B virus infection who was transferred to our hospital with a newly detected liver mass; abdominal computed tomography examination showed one hypodense mass of 7 cm in diameter and multiple mesenteric and mediastinal lymph nodes. A liver tumor biopsy showed a hepatoma, and the pathologic findings from an inguinal lymph node excision showed mantle cell lymphoma. An immunohistochemical stain confirmed that the atypical lymphoid cells within the HCC were positive for the CD20, CD5 and cyclin D1 antigens. Taking these findings into account, the hepatic tumor was determined to be a HCC infiltrated by mantle cell lymphoma.
文摘目的用48 h 快速培养获得的成熟树突细胞与肝癌细胞系 HCCLM3构建融合细胞疫苗。方法用 CD14正选磁珠从外周血中分离出CD14^+细胞,加入含有 GM-CSF 和 IL4的树突细胞完全培养基培养24 h,再加入肿瘤坏死因子(TNF)-α、IL-1β、IL-6和 PGE2,继续培养24 h 获得树突细胞并检测免疫分子 CD80、CD86、CD83和 HLA-DR 的表达。用50%聚乙二醇+10%二甲基亚砜融合 HCCLM3与所获树突细胞构建融合细胞并检测其免疫分子的表达。自体 T 细胞增殖实验按照刺激细胞不同分为融合细胞组(RH)、树突细胞组(DC)、HCCLM3组(H)及树突细胞与 HC-CLM3混合组(HH)。结果48 h 培养获得成熟树突细胞的 CD80、CD86、CD83和 HLA-DR 表达率可达94.43%、99.71%、62.78%和99.34%,它与 HCCLM3构建的融合细胞同样表达此类免疫分子,并且对自体 T 细胞增殖的刺激作用更强(P<0.05)。结论用48 h 快速培养获得的树突细胞可以成功构建能有效刺激自体 T 细胞增殖反应的融合细胞,该方法优点显著。
