AIM: To evaluate the predictive value of hepatocyte proliferation and hepatic angiogenesis for the occurrence of Hepatocellular carcinoma (HCC) in hepatitis C virus (HCV) cirrhotic patients.METHODS: One hundred-...AIM: To evaluate the predictive value of hepatocyte proliferation and hepatic angiogenesis for the occurrence of Hepatocellular carcinoma (HCC) in hepatitis C virus (HCV) cirrhotic patients.METHODS: One hundred-five patients (69 males, 36 females; age range, 51-90 year; median 66 year) with biopsy proven HCV cirrhosis were prospectively monitored for HCC occurrence for a median time of 64 too. Angiogenesis was assessed by using microvessel density (MVD), hepatocyte turnover by MIB1 and PCNA indexes at inclusion in liver biopsies.RESULTS: Forty six patients (43.8%) developed HCC after a median time of 55 (6-120) mo while 59 (56.2%) did not. Patients were divided into two groups according to the median value of each index. The difference between patients with low (median MVD = 3; range 0-20) and high (median MVD = 7; range 1-24) MVD was statist(caUy s(gn(ficant (χ^2= 22.06; P 〈 0.0001) wh(ch was not the case for MIB1 or PCNA (MIB-I: χ^2 = 1.41; P = 0.2351; PCNA: χ^2 = 1.27; P = 0.2589). The median MVD was higher in patients who developed HCC than in those who did not. HOe-free interval was significantly longer in patients with the MVD ≤ 4 (P = 0.0006). No relationship was found between MIB1 or PCNA and MVD (MIB-1 r^2 = 0.00007116, P = 0.9281; PCNA: P =0.001950; P = 0.6692). MVD only was able to predict the occurrence of HCC in these patients. Among other known risk factors for HCC, only male sex was statistically associated with an increased risk. CONCLUSION: Liver angiogenesis has a role for in HCV- related liver carcinogenesis and for defining patients at higher risk.展开更多
IM To compare the proliferating activity between HBVrelated small hepatocellular carcinoma (HCC) and HCVrelated small HCC.METHODS Sixty liver biopsy specimens from patients with small HCC (≤3cm in diameter) were ex...IM To compare the proliferating activity between HBVrelated small hepatocellular carcinoma (HCC) and HCVrelated small HCC.METHODS Sixty liver biopsy specimens from patients with small HCC (≤3cm in diameter) were examined immunohistochemically using antiproliferating cell nuclear antigen monoclonal antibody, of them 30 were HBVrelated HCC and 30 HCVrelated HCC with matched sex and morphologic features.RESULTS The labeling index of proliferating cell nuclear antigen was 79% in HBVrelated HCC and 125% in HCVrelated HCC. There was no statistically significant differences between the two groups (P>005).CONCLUSION In the early phase or small stage of hepatocellular carcinoma, HBVrelated HCC showed similar proliferating activity to that of HCVrelated HCC which suggested that in the early phase, HBVrelated HCC has similar malignancy to HCVrelated HCC.展开更多
Aim: We aimed to elucidate whether beta2-glycoprotein I (β2GPI) cooperation with hepatitis B surface antigen (HBsAg) promoted hepatocellular carcinogenesis enhanced by the lipopolysaccharide (LPS) via activation of n...Aim: We aimed to elucidate whether beta2-glycoprotein I (β2GPI) cooperation with hepatitis B surface antigen (HBsAg) promoted hepatocellular carcinogenesis enhanced by the lipopolysaccharide (LPS) via activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and expression of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), and alpha fetal protein (AFP) in liver cancer cells. Methods: Liver cancer cells (SMMC-7721) were transiently transfected with β2GPI and/or HBsAg and were subjected to LPS treatment. TNF-α, IL-1β, and AFP expression were measured in all groups by ELISA. NF-κB activation was assessed by non-radioactive electrophoretic mobility shift assay (EMSA) and was quantified in all groups. Results: Cells transfected with β2GPI and/or HBsAg induced activation of NF-κB, with the highest activation seen in the doubly β2GPI- and HBsAg-transfected cells treated with LPS. Non-transfected cells treated with LPS exhibited lower activation compared to either β2GPI- or HBsAg-transfected cells with LPS treatment. In addition, cells transfected with β2GPI and/or HBsAg induced significantly increased expression of TNF-α, IL-1β and AFP, with the highest levels again seen in the doubly β2GPI- and HBsAg-transfected cells treated with LPS. Conclusion: These observations suggest that the activity of NF-κB induced by β2GPI and HBsAg was enhanced by LPS. Expression of TNF-α, IL-1β and AFP increased in β2GPI and HBsAg cotransfected liver cancer cells.展开更多
基金Supported by Grants from the Italian Ministry of University, Scientific and Technological Research (MIUR, Progetto Nazionale cofinanziato COFIN No. 2002067115)the University of Florence to R. M.
文摘AIM: To evaluate the predictive value of hepatocyte proliferation and hepatic angiogenesis for the occurrence of Hepatocellular carcinoma (HCC) in hepatitis C virus (HCV) cirrhotic patients.METHODS: One hundred-five patients (69 males, 36 females; age range, 51-90 year; median 66 year) with biopsy proven HCV cirrhosis were prospectively monitored for HCC occurrence for a median time of 64 too. Angiogenesis was assessed by using microvessel density (MVD), hepatocyte turnover by MIB1 and PCNA indexes at inclusion in liver biopsies.RESULTS: Forty six patients (43.8%) developed HCC after a median time of 55 (6-120) mo while 59 (56.2%) did not. Patients were divided into two groups according to the median value of each index. The difference between patients with low (median MVD = 3; range 0-20) and high (median MVD = 7; range 1-24) MVD was statist(caUy s(gn(ficant (χ^2= 22.06; P 〈 0.0001) wh(ch was not the case for MIB1 or PCNA (MIB-I: χ^2 = 1.41; P = 0.2351; PCNA: χ^2 = 1.27; P = 0.2589). The median MVD was higher in patients who developed HCC than in those who did not. HOe-free interval was significantly longer in patients with the MVD ≤ 4 (P = 0.0006). No relationship was found between MIB1 or PCNA and MVD (MIB-1 r^2 = 0.00007116, P = 0.9281; PCNA: P =0.001950; P = 0.6692). MVD only was able to predict the occurrence of HCC in these patients. Among other known risk factors for HCC, only male sex was statistically associated with an increased risk. CONCLUSION: Liver angiogenesis has a role for in HCV- related liver carcinogenesis and for defining patients at higher risk.
文摘IM To compare the proliferating activity between HBVrelated small hepatocellular carcinoma (HCC) and HCVrelated small HCC.METHODS Sixty liver biopsy specimens from patients with small HCC (≤3cm in diameter) were examined immunohistochemically using antiproliferating cell nuclear antigen monoclonal antibody, of them 30 were HBVrelated HCC and 30 HCVrelated HCC with matched sex and morphologic features.RESULTS The labeling index of proliferating cell nuclear antigen was 79% in HBVrelated HCC and 125% in HCVrelated HCC. There was no statistically significant differences between the two groups (P>005).CONCLUSION In the early phase or small stage of hepatocellular carcinoma, HBVrelated HCC showed similar proliferating activity to that of HCVrelated HCC which suggested that in the early phase, HBVrelated HCC has similar malignancy to HCVrelated HCC.
文摘Aim: We aimed to elucidate whether beta2-glycoprotein I (β2GPI) cooperation with hepatitis B surface antigen (HBsAg) promoted hepatocellular carcinogenesis enhanced by the lipopolysaccharide (LPS) via activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and expression of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), and alpha fetal protein (AFP) in liver cancer cells. Methods: Liver cancer cells (SMMC-7721) were transiently transfected with β2GPI and/or HBsAg and were subjected to LPS treatment. TNF-α, IL-1β, and AFP expression were measured in all groups by ELISA. NF-κB activation was assessed by non-radioactive electrophoretic mobility shift assay (EMSA) and was quantified in all groups. Results: Cells transfected with β2GPI and/or HBsAg induced activation of NF-κB, with the highest activation seen in the doubly β2GPI- and HBsAg-transfected cells treated with LPS. Non-transfected cells treated with LPS exhibited lower activation compared to either β2GPI- or HBsAg-transfected cells with LPS treatment. In addition, cells transfected with β2GPI and/or HBsAg induced significantly increased expression of TNF-α, IL-1β and AFP, with the highest levels again seen in the doubly β2GPI- and HBsAg-transfected cells treated with LPS. Conclusion: These observations suggest that the activity of NF-κB induced by β2GPI and HBsAg was enhanced by LPS. Expression of TNF-α, IL-1β and AFP increased in β2GPI and HBsAg cotransfected liver cancer cells.