Aim: To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration. Methods: Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB p...Aim: To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration. Methods: Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes. Results: Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis B. In 9(9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots and the others 2 to 4 signals. The fluorescence intensity showed significant difference among the signal spots. The distribution of signal sites among chromosomes seems to be random. Conclusion: HBV could integrate into human sperm chromosomes. Results suggest that the possibility of vertical transmission of HBV via the germ line to the next generation is present.展开更多
To study the effect of different delivery modes on immunoprophylaxis efficacy so as to clarify whether or not cesarean section reduces immunoprophylaxis failure Methods Mothers with positive hepatitis B surface ant...To study the effect of different delivery modes on immunoprophylaxis efficacy so as to clarify whether or not cesarean section reduces immunoprophylaxis failure Methods Mothers with positive hepatitis B surface antigen (HBsAg) were selected in the third trimester of pregnancy Their babies were inoculated with hepatitis B immunoglobulin at birth and hepatitis B vaccine at 1, 2 and 7 months of age HBsAg and its antibodies (anti HBs) were tested at 1, 4, 7, and 12 months of age, then followed up yearly Results A total of 301 babies entered the study, including 144 born by normal spontaneous vaginal delivery, 40 by obstetric forceps or vacuum extraction, and 117 by cesarean section The incidence of mother’s HBeAg positivity or baby’s gender constitution was comparable between the three groups There were no significant differences in the positive rate of anti HBs or HBsAg at follow up periods among the three groups At 12 months of age, anti HBs could be detected in 78 9% of the babies born by normal vaginal delivery, 84 6% of the babies by forceps or vacuum extraction, and 86 4% of the babies by cesarean section The positive rate of HBsAg was 8 1%, 7 7%, 9 7%, and chronic HBV infection incidence was 7 3%, 7 7%, 6 8% respectively Conclusions There are no significant effects of delivery mode on the interruption of HBV maternal baby transmission by immunoprophylaxis Cesarean section does not reduce the incidence of immunoprophylaxis failure展开更多
文摘Aim: To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration. Methods: Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes. Results: Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis B. In 9(9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots and the others 2 to 4 signals. The fluorescence intensity showed significant difference among the signal spots. The distribution of signal sites among chromosomes seems to be random. Conclusion: HBV could integrate into human sperm chromosomes. Results suggest that the possibility of vertical transmission of HBV via the germ line to the next generation is present.
文摘To study the effect of different delivery modes on immunoprophylaxis efficacy so as to clarify whether or not cesarean section reduces immunoprophylaxis failure Methods Mothers with positive hepatitis B surface antigen (HBsAg) were selected in the third trimester of pregnancy Their babies were inoculated with hepatitis B immunoglobulin at birth and hepatitis B vaccine at 1, 2 and 7 months of age HBsAg and its antibodies (anti HBs) were tested at 1, 4, 7, and 12 months of age, then followed up yearly Results A total of 301 babies entered the study, including 144 born by normal spontaneous vaginal delivery, 40 by obstetric forceps or vacuum extraction, and 117 by cesarean section The incidence of mother’s HBeAg positivity or baby’s gender constitution was comparable between the three groups There were no significant differences in the positive rate of anti HBs or HBsAg at follow up periods among the three groups At 12 months of age, anti HBs could be detected in 78 9% of the babies born by normal vaginal delivery, 84 6% of the babies by forceps or vacuum extraction, and 86 4% of the babies by cesarean section The positive rate of HBsAg was 8 1%, 7 7%, 9 7%, and chronic HBV infection incidence was 7 3%, 7 7%, 6 8% respectively Conclusions There are no significant effects of delivery mode on the interruption of HBV maternal baby transmission by immunoprophylaxis Cesarean section does not reduce the incidence of immunoprophylaxis failure
