AIM:To evaluate the efficacy of antiviral or corticosteroid treatment on hepatitis B virus-associated glomerulonephritis(HBV-GN) . METHODS:Six and five trials were used respectively to evaluate the efficacy of either ...AIM:To evaluate the efficacy of antiviral or corticosteroid treatment on hepatitis B virus-associated glomerulonephritis(HBV-GN) . METHODS:Six and five trials were used respectively to evaluate the efficacy of either antiviral or corticosteroid treatment on HBV-GN.Pediatric patients were pooled separately to assess their response to the above treatment modalities.The primary and secondary outcomes were remission of proteinuria and clearance of Hepatitis B e-antigen(HBeAg) ,respectively.A fixed or random effect model was established to collect the data. RESULTS:The remission rate of proteinuria(RR=1.69,95%CI:1.08-2.65) and the clearance rate of HBeAg(RR =6.44,95%CI:3.11-13.35) were significantly higher in antiviral treatment group than in control group.The proteinuria remission was significantly associated with HBeAg clearance(P=0.002) .However,the difference in proteinuria remission rate was not statistically significant between corticosteroid treatment group and controlgroup(RR=1.45,95%CI:0.68-3.11) .Antiviral therapy could significantly promote the HBeAg clearance in pediatric patients,but neither antiviral nor corticosteroid therapy could significantly decrease proteinuria in pediatric patients compared to controls. CONCLUSION:Antiviral but not corticosteroid treatment can decrease proteinuria and promote HBeAg clearance in HBV-GN patients.展开更多
Objective To study the pathogenesis of hepatitis B virus ( HBV ) on kidney tissues. Methods HBsAg and HBcAg in paraffin-embedded renal biopsy tissues from 27 cases of glomerulonephritis with positive serum HBV markers...Objective To study the pathogenesis of hepatitis B virus ( HBV ) on kidney tissues. Methods HBsAg and HBcAg in paraffin-embedded renal biopsy tissues from 27 cases of glomerulonephritis with positive serum HBV markers were observed by using immunohistochemistry. In addition, in situ polymerse chain reaction (IS-PCR) was performed in 5 cases with positive HBsAg and HBcAg in renal tissue of the 27-case glomerulonephritis to reveal the state of renal HBV DNA. Results Twenty cases (20/27,74.07%) were positive with HBAg which were mainly diffusely distributed in epithelial cells of renal tubule. Four cases (4/5,80% ) were positive with HBV DNA whose distribution was the same of that of HBAg. Conclusion Renal lesions due to HBV are not only the results of immunologic response, but also the outcome of direct invasion and duplication of HBV in epithelial cells of renal tubule.展开更多
目的探讨HBV相关性肾炎(hepatitis B virus associated glomerulonephritis,HBV-GN)易感性与维生素D受体(vitamin D receptor,VDR)基因BsmⅠ及FokⅠ酶切位点多态性的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技...目的探讨HBV相关性肾炎(hepatitis B virus associated glomerulonephritis,HBV-GN)易感性与维生素D受体(vitamin D receptor,VDR)基因BsmⅠ及FokⅠ酶切位点多态性的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术检测VDR基因BsmⅠ及FokⅠ的多态性在40例HBV-GN患者、34例慢性乙肝患者及46例健康对照者中的分布,并进行基因型分析。结果 HBV-GN组FF基因型分布明显低于正常对照组及慢性乙肝组(χ2=9.084、10.254,P=0.0025、0.0014),HBV-GN组F/f等位基因频率分布和对照组及慢性乙肝组比较无统计学意义(P>0.05);慢性乙肝组FokⅠ位点基因型和等位基因频率分布与正常对照组比较差异不显著(P>0.05)。HBV-GN组和乙型肝炎组Bb基因型分布明显高于正常对照组(χ2=8.9451、0.435,P=0.0027、0.0012),两组B等位基因分布明显高于对照组(χ2=7.9001、0.329,P=0.00490、.0013);HBV-GN和慢性乙肝组间比较差异不显著。结论 VDR基因多态性可能与乙肝相关性肾炎及HBV感染相关联。展开更多
目的探讨乙型肝炎病毒相关性肾炎(hepatitis B virus-associated glomerulonephritis,HBV-GN)易感性与乙型肝炎病毒(HBV)CP区基因变异的关系。方法选择19例HBV-GN为实验组,22例慢性HBV感染者为对照组,应用基因测序技术检测HBV血浆CP区...目的探讨乙型肝炎病毒相关性肾炎(hepatitis B virus-associated glomerulonephritis,HBV-GN)易感性与乙型肝炎病毒(HBV)CP区基因变异的关系。方法选择19例HBV-GN为实验组,22例慢性HBV感染者为对照组,应用基因测序技术检测HBV血浆CP区基因位点的变异。结果 CP区中nt1727和nt1773位点HBV-GN组的变异率显著高于HBV组(100%和59.1%,47.4%和13.6%,P<0.05)。nt1762/1764联合变异率在两组中的差别无统计学意义(P>0.05)。结论 HBV-GN易感性可能与HBV的CP区基因变异有关。展开更多
基金Supported by National Natural Science Foundation of China,No.30772360
文摘AIM:To evaluate the efficacy of antiviral or corticosteroid treatment on hepatitis B virus-associated glomerulonephritis(HBV-GN) . METHODS:Six and five trials were used respectively to evaluate the efficacy of either antiviral or corticosteroid treatment on HBV-GN.Pediatric patients were pooled separately to assess their response to the above treatment modalities.The primary and secondary outcomes were remission of proteinuria and clearance of Hepatitis B e-antigen(HBeAg) ,respectively.A fixed or random effect model was established to collect the data. RESULTS:The remission rate of proteinuria(RR=1.69,95%CI:1.08-2.65) and the clearance rate of HBeAg(RR =6.44,95%CI:3.11-13.35) were significantly higher in antiviral treatment group than in control group.The proteinuria remission was significantly associated with HBeAg clearance(P=0.002) .However,the difference in proteinuria remission rate was not statistically significant between corticosteroid treatment group and controlgroup(RR=1.45,95%CI:0.68-3.11) .Antiviral therapy could significantly promote the HBeAg clearance in pediatric patients,but neither antiviral nor corticosteroid therapy could significantly decrease proteinuria in pediatric patients compared to controls. CONCLUSION:Antiviral but not corticosteroid treatment can decrease proteinuria and promote HBeAg clearance in HBV-GN patients.
基金ThisworkwassupportedbytheNaturalScienceFoundationofShaanxiProvince (No .2 0 0 0SM48) .
文摘Objective To study the pathogenesis of hepatitis B virus ( HBV ) on kidney tissues. Methods HBsAg and HBcAg in paraffin-embedded renal biopsy tissues from 27 cases of glomerulonephritis with positive serum HBV markers were observed by using immunohistochemistry. In addition, in situ polymerse chain reaction (IS-PCR) was performed in 5 cases with positive HBsAg and HBcAg in renal tissue of the 27-case glomerulonephritis to reveal the state of renal HBV DNA. Results Twenty cases (20/27,74.07%) were positive with HBAg which were mainly diffusely distributed in epithelial cells of renal tubule. Four cases (4/5,80% ) were positive with HBV DNA whose distribution was the same of that of HBAg. Conclusion Renal lesions due to HBV are not only the results of immunologic response, but also the outcome of direct invasion and duplication of HBV in epithelial cells of renal tubule.
文摘目的探讨HBV相关性肾炎(hepatitis B virus associated glomerulonephritis,HBV-GN)易感性与维生素D受体(vitamin D receptor,VDR)基因BsmⅠ及FokⅠ酶切位点多态性的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术检测VDR基因BsmⅠ及FokⅠ的多态性在40例HBV-GN患者、34例慢性乙肝患者及46例健康对照者中的分布,并进行基因型分析。结果 HBV-GN组FF基因型分布明显低于正常对照组及慢性乙肝组(χ2=9.084、10.254,P=0.0025、0.0014),HBV-GN组F/f等位基因频率分布和对照组及慢性乙肝组比较无统计学意义(P>0.05);慢性乙肝组FokⅠ位点基因型和等位基因频率分布与正常对照组比较差异不显著(P>0.05)。HBV-GN组和乙型肝炎组Bb基因型分布明显高于正常对照组(χ2=8.9451、0.435,P=0.0027、0.0012),两组B等位基因分布明显高于对照组(χ2=7.9001、0.329,P=0.00490、.0013);HBV-GN和慢性乙肝组间比较差异不显著。结论 VDR基因多态性可能与乙肝相关性肾炎及HBV感染相关联。
