To study the effects of parthenolide on human hepatocellular carcinoma cell line BEL-7402 and explore its possible mechanism, human hepatocellular carcinoma cell line BEL-7402 was cultured in vitro and treated with pa...To study the effects of parthenolide on human hepatocellular carcinoma cell line BEL-7402 and explore its possible mechanism, human hepatocellular carcinoma cell line BEL-7402 was cultured in vitro and treated with parthenolide of different concentrations. Cells without addition of parthenolide were used as control. The growth of inhibition of cells induced by various concentrations was analyzed by using the MTT assay. The morphologic changes of apoptosis was observed under an inversion microscope by Giemsa staining. The expression levels of PCNA albumen were measured by means of immunohistochemical methods. Parthenolide can inhibit the proliferation of BEL-7402 cells in vitro in adose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage were found by means of inversion microscopy. Formation of apoptotic bodies was observed by Giemsa staining. The immunohistochemical results show that the expression of PCNA has been decreased. Parthenolide can inhibit the tumor growing of BEL-7402 cells, and induce the apoptosis of cells. The mechanisms of those functions may be via inhibiting the expression of PCNA.展开更多
Objective： To observe the clinical effects of kanglaite （KLT） capsules combined with transcatheter arterial chemoembolization （TACE） in treating patients with mid or late-stage primary hepatocellular carcinoma （...Objective： To observe the clinical effects of kanglaite （KLT） capsules combined with transcatheter arterial chemoembolization （TACE） in treating patients with mid or late-stage primary hepatocellular carcinoma （HCC）. Methods： Sixty-five cases were randomly divided into 2 groups, 32 patients in combination group received the treatment of KLT capsules ＋ TACE and 33 patients in control group were treated with TACE alone. The objective response rate （RR）, serum alpha fetoprotein （AFP）, peripheral blood T lymphocyte subgroups （T-LS）, quality of life （QOL）, time to progression （TTP） and adverse reaction were observed and compared between 2 groups. Results： The objective response rate and serum alpha fetoprotein levels had no significant difference between the two groups （P 〉 0.05）. Combination group was superior to control group in quality of life （QOL）, time to progression （TTP）, peripheral blood T lymphocyte subgroups （CD3＋, CD4＋, CD4＋／CD8 ratio） and liver adverse reactions, with significant differences （P 〈 0.05）. Conclusion： KLT capsules combined with TACE is an effective method to treat primary hepatocellular carcinoma （HCC） patients who have lost the opportunity of surgical therapy.展开更多
Liver cancer is a prevalent malignant cancer,ranking third in terms of mortality rate.Metastasis and recurrence primarily contribute to the high mortality rate of liver cancer.Hepatocellular carcinoma(HCC)has low expr...Liver cancer is a prevalent malignant cancer,ranking third in terms of mortality rate.Metastasis and recurrence primarily contribute to the high mortality rate of liver cancer.Hepatocellular carcinoma(HCC)has low expression of focal adhesion kinase(FAK),which increases the risk of metastasis and recurrence.Nevertheless,the efficacy of FAK phosphorylation inhibitors is currently limited.Thus,investigating the mechanisms by which FAK affects HCC metastasis to develop targeted therapies for FAK may present a novel strategy to inhibit HCC metastasis.This study examined the correlation between FAK expression and the prognosis of HCC.Additionally,we explored the impact of FAK degradation on HCC metastasis through wound healing experiments,transwell invasion experiments,and a xenograft tumor model.The expression of proteins related to epithelial-mesenchymal transition(EMT)was measured to elucidate the underlying mechanisms.The results showed that FAK PROTAC can degrade FAK,inhibit the migration and invasion of HCC cells in vitro,and notably decrease the lung metastasis of HCC in vivo.Increased expression of E-cadherin and decreased expression of vimentin indicated that EMT was inhibited.Consequently,degradation of FAK through FAK PROTAC effectively suppressed liver cancer metastasis,holding significant clinical implications for treating liver cancer and developing innovative anti-neoplastic drugs.展开更多
Background:The role of TROVE domain family member 2(TROVE2)has been well-demonstrated in autoimmune diseases;however,its involvement in liver cancer remains unclear.Therefore,this study aimed to explore the biological...Background:The role of TROVE domain family member 2(TROVE2)has been well-demonstrated in autoimmune diseases;however,its involvement in liver cancer remains unclear.Therefore,this study aimed to explore the biological function and clinical significance of TROVE2 in hepatocellular carcinoma(HCC).Methods:The expression level of TROVE2 was analyzed in HCC and paired adjacent tissue samples using real-time reverse transcription-quantitative polymerase chain reaction.The impact of TROVE2 on migration and invasion in HCC cells was analyzed through Transwell assays and Western blotting.High-throughput transcriptome sequencing and bioinformatics analyses were performed to identify downstream target genes.Back-complementation experiments were employed to verify the influence of downstream proteins on TROVE2-induced invasion and migration of HCC cells.Results:TROVE2 exhibited significant overexpression in liver cancer tissue,correlating with shorter overall survival.Overexpression of TROVE2 facilitated the invasion,metastasis,and epithelial-mesenchymal transition(EMT)process of HCC cells,whereas TROVE2 knockdown restrained migration,invasion,and EMT in these cells.Transcriptome sequencing and bioinformatics analysis identified heparanase(HPSE)as a downstreamtarget protein of TROVE2.Subsequent back-complementation experiments provided evidence that HPSE overexpression promoted TROVE2-mediated prometastasis effects.Moreover,the study revealed that TROVE2 was capable of regulating the EMT pathway through GSK-3βphosphorylation.Conclusions:TROVE2 facilitated the invasion,migration,and EMT process ofHCC cells through phosphorylation of the HPSE/GSK-3βaxis,indicating its significance as an important protein in tumor progression.展开更多
This article reviews the concept and clinical manifestations of post embolism syndrome after transarterial chemoembolization(TACE),and the prevention or timely intervention of post embolism syndrome in advance is expe...This article reviews the concept and clinical manifestations of post embolism syndrome after transarterial chemoembolization(TACE),and the prevention or timely intervention of post embolism syndrome in advance is expected to reduce its incidence and degree in clinical treatment,and to improve the quality of treatment of Hepatocellular Carcinoma Carcinoma(HCC).展开更多
Introduction: Acute hemoperitoneum due to the spontaneous rupture of hepatocellular carcinoma (HCC) is a rare case of non-traumatic intra-abdomen bleeding that requires a high index of suspicion to approach, especiall...Introduction: Acute hemoperitoneum due to the spontaneous rupture of hepatocellular carcinoma (HCC) is a rare case of non-traumatic intra-abdomen bleeding that requires a high index of suspicion to approach, especially if no known history of HCC. It can mislead the physicians when the patient presents in an atypical way. Case Presentation: In this case report, we describe a fortuitous rupture of hepatocellular carcinoma in a 58-year-old male who was not previously diagnosed as having HCC and who came with atypical symptoms and signs of hemoperitoneum. He was then treated by trans-arterial embolectomy. Discussion: Diagnosis of hemoperitoneum in a case with bradycardia and hypotension is uncommon, as it goes more towards cardiogenic shock than hypovolemic shock, especially in a patient who is previously not symptomatic and has no risk factor for hepatocellular carcinoma. Conclusion: physicians should be alert to the possibility of encountering a hemorrhagic shock, although no trauma injury in any hypotensive patient with no clear reason for his condition.展开更多
Hepatocellular carcinoma is prone to invasion and metastasis.It often receives a low diagnosis rate in the early stage but has an extremely high mortality rate.Epithelial-mesenchymal transformation(EMT)is a key factor...Hepatocellular carcinoma is prone to invasion and metastasis.It often receives a low diagnosis rate in the early stage but has an extremely high mortality rate.Epithelial-mesenchymal transformation(EMT)is a key factor in promoting tumor cell invasion and metastasis.Circular RNA(circRNA)is involved in regulating EMT in hepatocarcinoma cells through multiple pathways,thereby affecting the occurrence and progression of hepatocellular carcinoma.This article mainly reviews the research progress of circRNA related to EMT core transcription factors,circRNA that promotes EMT in liver cancer,and circRNA that inhibits EMT in liver cancer.展开更多
目的探讨磁共振(magnetic resonance imaging,MRI)扩散峰度成像(diffusion kurtosis imaging,DKI)和拉伸指数模型(stretched exponential model,SEM)评估自然生长状态下裸鼠原位肝细胞癌(hepatocellular carcinoma,HCC)空间和时间异质...目的探讨磁共振(magnetic resonance imaging,MRI)扩散峰度成像(diffusion kurtosis imaging,DKI)和拉伸指数模型(stretched exponential model,SEM)评估自然生长状态下裸鼠原位肝细胞癌(hepatocellular carcinoma,HCC)空间和时间异质性的价值。方法将25只原位HCC裸鼠模型随机分为A、B、C、D和E组,每组5只,分别于原位种植瘤生长至第21、28、35、42和49天进行1.5T MRI扫描,获得DKI和SEM以下各参数:平均峰度(mean kurtosis,MK)、平均扩散系数(mean diffusivity,MD)、扩散异质性指数α和扩散分布指数(distributed diffusion coefficient,DDC)。使用Kruskal-Wallis H 检验和Mann-Whitney U 检验比较各组间肿瘤DKI和SEM各参数的差异。使用Spearman相关分析评价DKI和SEM各参数与组织病理学上坏死分数(necrotic fraction,NF)、微血管密度(micro-vessel density,MVD)、肿瘤细胞增殖指标Ki-67指数、HE染色肿瘤最大径切面直方图异质性指标的标准差(standard deviation,SD)和峰度以及肿瘤大小之间的相关性。结果各组间肿瘤MK、MD、α和DDC值差异均有显著统计学意义( P 均<0.05)。D组肿瘤MK值明显高于A、B和E组( P 均<0.05),α值明显低于A、B和E组( P 均<0.05)。D组肿瘤MD和DDC值显著高于B组( P 均< 0.05 ),E组显著高于B、C组( P 均<0.05)。5组肿瘤的MK值与SD和峰度值高度正相关( r =0.603和0.604, P 均<0.05),α值与SD和峰度值高度负相关( r =-0.627和-0.620, P 均<0.05)。从第21天至第42天肿瘤MK值与NF( r =0.587, P =0.006)、MVD( r =0.490, P =0.028)、Ki-67指数( r =0.569, P = 0.009 )和肿瘤大小( r =0.503, P =0.024)中度正相关,α值与NF( r =-0.577, P =0.008)、MVD( r =-0.490, P = 0.028)、Ki-67指数( r =-0.574, P =0.008)和肿瘤大小( r =-0.488, P =0.029)中度负相关。从第28至第49天肿瘤MD和DDC值与NF高度正相关( r =0.706和0.664, P 均<0.05),与肿瘤大小中度正相关( r =0.492和0.525, P 均<0.05)。结论DKI和SEM或可成为临床无创性评价HCC时间和空间异质性的生物影像学指标。展开更多
文摘To study the effects of parthenolide on human hepatocellular carcinoma cell line BEL-7402 and explore its possible mechanism, human hepatocellular carcinoma cell line BEL-7402 was cultured in vitro and treated with parthenolide of different concentrations. Cells without addition of parthenolide were used as control. The growth of inhibition of cells induced by various concentrations was analyzed by using the MTT assay. The morphologic changes of apoptosis was observed under an inversion microscope by Giemsa staining. The expression levels of PCNA albumen were measured by means of immunohistochemical methods. Parthenolide can inhibit the proliferation of BEL-7402 cells in vitro in adose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage were found by means of inversion microscopy. Formation of apoptotic bodies was observed by Giemsa staining. The immunohistochemical results show that the expression of PCNA has been decreased. Parthenolide can inhibit the tumor growing of BEL-7402 cells, and induce the apoptosis of cells. The mechanisms of those functions may be via inhibiting the expression of PCNA.
文摘Objective： To observe the clinical effects of kanglaite （KLT） capsules combined with transcatheter arterial chemoembolization （TACE） in treating patients with mid or late-stage primary hepatocellular carcinoma （HCC）. Methods： Sixty-five cases were randomly divided into 2 groups, 32 patients in combination group received the treatment of KLT capsules ＋ TACE and 33 patients in control group were treated with TACE alone. The objective response rate （RR）, serum alpha fetoprotein （AFP）, peripheral blood T lymphocyte subgroups （T-LS）, quality of life （QOL）, time to progression （TTP） and adverse reaction were observed and compared between 2 groups. Results： The objective response rate and serum alpha fetoprotein levels had no significant difference between the two groups （P 〉 0.05）. Combination group was superior to control group in quality of life （QOL）, time to progression （TTP）, peripheral blood T lymphocyte subgroups （CD3＋, CD4＋, CD4＋／CD8 ratio） and liver adverse reactions, with significant differences （P 〈 0.05）. Conclusion： KLT capsules combined with TACE is an effective method to treat primary hepatocellular carcinoma （HCC） patients who have lost the opportunity of surgical therapy.
基金supported by the National Natural Science Foundation of China Fund Project(82272956).
文摘Liver cancer is a prevalent malignant cancer,ranking third in terms of mortality rate.Metastasis and recurrence primarily contribute to the high mortality rate of liver cancer.Hepatocellular carcinoma(HCC)has low expression of focal adhesion kinase(FAK),which increases the risk of metastasis and recurrence.Nevertheless,the efficacy of FAK phosphorylation inhibitors is currently limited.Thus,investigating the mechanisms by which FAK affects HCC metastasis to develop targeted therapies for FAK may present a novel strategy to inhibit HCC metastasis.This study examined the correlation between FAK expression and the prognosis of HCC.Additionally,we explored the impact of FAK degradation on HCC metastasis through wound healing experiments,transwell invasion experiments,and a xenograft tumor model.The expression of proteins related to epithelial-mesenchymal transition(EMT)was measured to elucidate the underlying mechanisms.The results showed that FAK PROTAC can degrade FAK,inhibit the migration and invasion of HCC cells in vitro,and notably decrease the lung metastasis of HCC in vivo.Increased expression of E-cadherin and decreased expression of vimentin indicated that EMT was inhibited.Consequently,degradation of FAK through FAK PROTAC effectively suppressed liver cancer metastasis,holding significant clinical implications for treating liver cancer and developing innovative anti-neoplastic drugs.
基金the Natural Science Foundation of Fujian Province(2021 J01539,2023 J011467)Scientific Foundation of the Fuzhou Health Commission(2021-S-wq21,2021-S-wp1).
文摘Background:The role of TROVE domain family member 2(TROVE2)has been well-demonstrated in autoimmune diseases;however,its involvement in liver cancer remains unclear.Therefore,this study aimed to explore the biological function and clinical significance of TROVE2 in hepatocellular carcinoma(HCC).Methods:The expression level of TROVE2 was analyzed in HCC and paired adjacent tissue samples using real-time reverse transcription-quantitative polymerase chain reaction.The impact of TROVE2 on migration and invasion in HCC cells was analyzed through Transwell assays and Western blotting.High-throughput transcriptome sequencing and bioinformatics analyses were performed to identify downstream target genes.Back-complementation experiments were employed to verify the influence of downstream proteins on TROVE2-induced invasion and migration of HCC cells.Results:TROVE2 exhibited significant overexpression in liver cancer tissue,correlating with shorter overall survival.Overexpression of TROVE2 facilitated the invasion,metastasis,and epithelial-mesenchymal transition(EMT)process of HCC cells,whereas TROVE2 knockdown restrained migration,invasion,and EMT in these cells.Transcriptome sequencing and bioinformatics analysis identified heparanase(HPSE)as a downstreamtarget protein of TROVE2.Subsequent back-complementation experiments provided evidence that HPSE overexpression promoted TROVE2-mediated prometastasis effects.Moreover,the study revealed that TROVE2 was capable of regulating the EMT pathway through GSK-3βphosphorylation.Conclusions:TROVE2 facilitated the invasion,migration,and EMT process ofHCC cells through phosphorylation of the HPSE/GSK-3βaxis,indicating its significance as an important protein in tumor progression.
文摘This article reviews the concept and clinical manifestations of post embolism syndrome after transarterial chemoembolization(TACE),and the prevention or timely intervention of post embolism syndrome in advance is expected to reduce its incidence and degree in clinical treatment,and to improve the quality of treatment of Hepatocellular Carcinoma Carcinoma(HCC).
文摘Introduction: Acute hemoperitoneum due to the spontaneous rupture of hepatocellular carcinoma (HCC) is a rare case of non-traumatic intra-abdomen bleeding that requires a high index of suspicion to approach, especially if no known history of HCC. It can mislead the physicians when the patient presents in an atypical way. Case Presentation: In this case report, we describe a fortuitous rupture of hepatocellular carcinoma in a 58-year-old male who was not previously diagnosed as having HCC and who came with atypical symptoms and signs of hemoperitoneum. He was then treated by trans-arterial embolectomy. Discussion: Diagnosis of hemoperitoneum in a case with bradycardia and hypotension is uncommon, as it goes more towards cardiogenic shock than hypovolemic shock, especially in a patient who is previously not symptomatic and has no risk factor for hepatocellular carcinoma. Conclusion: physicians should be alert to the possibility of encountering a hemorrhagic shock, although no trauma injury in any hypotensive patient with no clear reason for his condition.
基金Medical Research Project of Xi’an Science and Technology Bureau(Project No.22YXYJ0134)。
文摘Hepatocellular carcinoma is prone to invasion and metastasis.It often receives a low diagnosis rate in the early stage but has an extremely high mortality rate.Epithelial-mesenchymal transformation(EMT)is a key factor in promoting tumor cell invasion and metastasis.Circular RNA(circRNA)is involved in regulating EMT in hepatocarcinoma cells through multiple pathways,thereby affecting the occurrence and progression of hepatocellular carcinoma.This article mainly reviews the research progress of circRNA related to EMT core transcription factors,circRNA that promotes EMT in liver cancer,and circRNA that inhibits EMT in liver cancer.
文摘目的探讨磁共振(magnetic resonance imaging,MRI)扩散峰度成像(diffusion kurtosis imaging,DKI)和拉伸指数模型(stretched exponential model,SEM)评估自然生长状态下裸鼠原位肝细胞癌(hepatocellular carcinoma,HCC)空间和时间异质性的价值。方法将25只原位HCC裸鼠模型随机分为A、B、C、D和E组,每组5只,分别于原位种植瘤生长至第21、28、35、42和49天进行1.5T MRI扫描,获得DKI和SEM以下各参数:平均峰度(mean kurtosis,MK)、平均扩散系数(mean diffusivity,MD)、扩散异质性指数α和扩散分布指数(distributed diffusion coefficient,DDC)。使用Kruskal-Wallis H 检验和Mann-Whitney U 检验比较各组间肿瘤DKI和SEM各参数的差异。使用Spearman相关分析评价DKI和SEM各参数与组织病理学上坏死分数(necrotic fraction,NF)、微血管密度(micro-vessel density,MVD)、肿瘤细胞增殖指标Ki-67指数、HE染色肿瘤最大径切面直方图异质性指标的标准差(standard deviation,SD)和峰度以及肿瘤大小之间的相关性。结果各组间肿瘤MK、MD、α和DDC值差异均有显著统计学意义( P 均<0.05)。D组肿瘤MK值明显高于A、B和E组( P 均<0.05),α值明显低于A、B和E组( P 均<0.05)。D组肿瘤MD和DDC值显著高于B组( P 均< 0.05 ),E组显著高于B、C组( P 均<0.05)。5组肿瘤的MK值与SD和峰度值高度正相关( r =0.603和0.604, P 均<0.05),α值与SD和峰度值高度负相关( r =-0.627和-0.620, P 均<0.05)。从第21天至第42天肿瘤MK值与NF( r =0.587, P =0.006)、MVD( r =0.490, P =0.028)、Ki-67指数( r =0.569, P = 0.009 )和肿瘤大小( r =0.503, P =0.024)中度正相关,α值与NF( r =-0.577, P =0.008)、MVD( r =-0.490, P = 0.028)、Ki-67指数( r =-0.574, P =0.008)和肿瘤大小( r =-0.488, P =0.029)中度负相关。从第28至第49天肿瘤MD和DDC值与NF高度正相关( r =0.706和0.664, P 均<0.05),与肿瘤大小中度正相关( r =0.492和0.525, P 均<0.05)。结论DKI和SEM或可成为临床无创性评价HCC时间和空间异质性的生物影像学指标。
