Objective:To develop an improved protocol for micropropagation of ethnomedicinally important Scoparia dulcis(S.dulcis) L.Methods:Explants were inoculated on MS basal medium supplemented with kinelin and 6-benzylaminop...Objective:To develop an improved protocol for micropropagation of ethnomedicinally important Scoparia dulcis(S.dulcis) L.Methods:Explants were inoculated on MS basal medium supplemented with kinelin and 6-benzylaminopurine for shoot bud induction.To enhance the shoot induction,various auxins like 3-indoleacetic acid or 3-indolebutyric acid or a-naphthylacetic acid were tested along with 2.32 M KI and 4.44 μ M BAP.The regenerated shoots were rooted in half strength MS medium supplemented with various concentrations of IAA,IBA or NAA.After roots were developed,the plantlets were transplanted to pots filled with vermiculate and sand and kept in growth chamber with 70%-80%humidity under 16 h photoperiod.After acclimatization,the plantlets were transferred to the garden and survival percentage was calculated.Data were statistically analyzed and means were compared using Duncan's multiple range test(P<0.05).Results:An in vitro method was developed to induce high frequency shoots regeneration from stem,mature leaf and young leaf explants of S.dulcis.Shoot induction on young leaf explants was most successful in MS medium supplemented with combination of two cytokinins(2.32 μ M KI and 4.44 μ M BAP) 2.85 μ M IAA,10%CM and 1 483.79 μM adenine sulfate.A single young leaf explant was capable of producing 59 shoots after 13 days of culture. Flower was induced in medium supplemented with combination of KI and BAP.Conclusions: Cytokinins are the key factor to induce the direct shoot regeneration and flowering of S.dulcis.展开更多
Primula tibetica is an inconspicuous herb distributed in the Himalayas where lacks pollinators.The species may undergo severe pollen limitation in the field,and makes an ideal model system for studying whether pollen ...Primula tibetica is an inconspicuous herb distributed in the Himalayas where lacks pollinators.The species may undergo severe pollen limitation in the field,and makes an ideal model system for studying whether pollen limitation selects on the traits that increase the floral display in a case in which a small,inconspicuous plant undergoes展开更多
在栽植前采用0(CK)、60、120和180 mg·L-16-BA、GA3和乙烯利对石蒜〔Lycoris radiata(L’Hér.)Herb.〕和换锦花(L.sprengeri Comes ex Baker)鳞茎进行浸球处理,对花芽分化期内(4月至7月)石蒜和换锦花鳞茎中可溶性糖、可溶性...在栽植前采用0(CK)、60、120和180 mg·L-16-BA、GA3和乙烯利对石蒜〔Lycoris radiata(L’Hér.)Herb.〕和换锦花(L.sprengeri Comes ex Baker)鳞茎进行浸球处理,对花芽分化期内(4月至7月)石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量的变化进行了比较分析。结果表明:在花芽分化期内,随时间的推移,对照组石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量呈先增加后降低的趋势,并在6月25日(心皮分化期)或7月10日(雌蕊分化期)达到最高值。经60、120和180 mg·L-16-BA、GA3和乙烯利浸球处理后,石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量在花芽分化期内先逐渐增加,至6月25日或达到峰值后开始下降或继续升高;但与对照相比,随时间的推移,石蒜鳞茎中可溶性糖含量总体上有不同程度的增加,而换锦花鳞茎中可溶性糖含量或高或低无规律性的变化,但各处理组石蒜和换锦花鳞茎中可溶性糖含量均与对照间有差异;可溶性蛋白质含量在进入花原基形成期(5月10日)后始终维持在较高的水平;核酸含量在花芽分化期的前期维持在较低的水平,在雌蕊分化期迅速增加并达到峰值,之后又急剧下降。总体上看,石蒜鳞茎中可溶性糖含量高于换锦花,可溶性蛋白质含量低于换锦花,而二者间核酸含量差异不明显。研究结果表明:3种外源植物生长调节剂浸球处理可在一定程度上对花芽分化期内石蒜和换锦花鳞茎的新陈代谢产生影响,且对石蒜的影响强于换锦花。展开更多
对红花酢浆草、草茉莉、中国石竹3种乡土观花草本植物在3种不同人工栽培处理状态下的部分生长特性进行了研究。结果表明,草茉莉和中国石竹作地被植物适合20 cm×20 cm 3株拼成1株整体移植的方式;分枝部位主要集中在3株植物的外缘,...对红花酢浆草、草茉莉、中国石竹3种乡土观花草本植物在3种不同人工栽培处理状态下的部分生长特性进行了研究。结果表明,草茉莉和中国石竹作地被植物适合20 cm×20 cm 3株拼成1株整体移植的方式;分枝部位主要集中在3株植物的外缘,植物表现整齐紧凑,群体观赏效果强、盛花期延长,开花集中。红花酢浆草作地被植物适合露地撒播的方式;植株覆盖力、紧凑性强。展开更多
基金Supported by a grant from UGC-New Delhi(No.MRP 3011/09)
文摘Objective:To develop an improved protocol for micropropagation of ethnomedicinally important Scoparia dulcis(S.dulcis) L.Methods:Explants were inoculated on MS basal medium supplemented with kinelin and 6-benzylaminopurine for shoot bud induction.To enhance the shoot induction,various auxins like 3-indoleacetic acid or 3-indolebutyric acid or a-naphthylacetic acid were tested along with 2.32 M KI and 4.44 μ M BAP.The regenerated shoots were rooted in half strength MS medium supplemented with various concentrations of IAA,IBA or NAA.After roots were developed,the plantlets were transplanted to pots filled with vermiculate and sand and kept in growth chamber with 70%-80%humidity under 16 h photoperiod.After acclimatization,the plantlets were transferred to the garden and survival percentage was calculated.Data were statistically analyzed and means were compared using Duncan's multiple range test(P<0.05).Results:An in vitro method was developed to induce high frequency shoots regeneration from stem,mature leaf and young leaf explants of S.dulcis.Shoot induction on young leaf explants was most successful in MS medium supplemented with combination of two cytokinins(2.32 μ M KI and 4.44 μ M BAP) 2.85 μ M IAA,10%CM and 1 483.79 μM adenine sulfate.A single young leaf explant was capable of producing 59 shoots after 13 days of culture. Flower was induced in medium supplemented with combination of KI and BAP.Conclusions: Cytokinins are the key factor to induce the direct shoot regeneration and flowering of S.dulcis.
基金funded by the National Natural Science Foundation of Chinathe Chinese Academy of SciencesYunnan provincial government
文摘Primula tibetica is an inconspicuous herb distributed in the Himalayas where lacks pollinators.The species may undergo severe pollen limitation in the field,and makes an ideal model system for studying whether pollen limitation selects on the traits that increase the floral display in a case in which a small,inconspicuous plant undergoes
文摘在栽植前采用0(CK)、60、120和180 mg·L-16-BA、GA3和乙烯利对石蒜〔Lycoris radiata(L’Hér.)Herb.〕和换锦花(L.sprengeri Comes ex Baker)鳞茎进行浸球处理,对花芽分化期内(4月至7月)石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量的变化进行了比较分析。结果表明:在花芽分化期内,随时间的推移,对照组石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量呈先增加后降低的趋势,并在6月25日(心皮分化期)或7月10日(雌蕊分化期)达到最高值。经60、120和180 mg·L-16-BA、GA3和乙烯利浸球处理后,石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量在花芽分化期内先逐渐增加,至6月25日或达到峰值后开始下降或继续升高;但与对照相比,随时间的推移,石蒜鳞茎中可溶性糖含量总体上有不同程度的增加,而换锦花鳞茎中可溶性糖含量或高或低无规律性的变化,但各处理组石蒜和换锦花鳞茎中可溶性糖含量均与对照间有差异;可溶性蛋白质含量在进入花原基形成期(5月10日)后始终维持在较高的水平;核酸含量在花芽分化期的前期维持在较低的水平,在雌蕊分化期迅速增加并达到峰值,之后又急剧下降。总体上看,石蒜鳞茎中可溶性糖含量高于换锦花,可溶性蛋白质含量低于换锦花,而二者间核酸含量差异不明显。研究结果表明:3种外源植物生长调节剂浸球处理可在一定程度上对花芽分化期内石蒜和换锦花鳞茎的新陈代谢产生影响,且对石蒜的影响强于换锦花。
文摘对红花酢浆草、草茉莉、中国石竹3种乡土观花草本植物在3种不同人工栽培处理状态下的部分生长特性进行了研究。结果表明,草茉莉和中国石竹作地被植物适合20 cm×20 cm 3株拼成1株整体移植的方式;分枝部位主要集中在3株植物的外缘,植物表现整齐紧凑,群体观赏效果强、盛花期延长,开花集中。红花酢浆草作地被植物适合露地撒播的方式;植株覆盖力、紧凑性强。
