Objective:To review the possible mechanism and clinical application of the main components of Yuxingcao(Herba Houttuyniae)in the treatment of diabetic kidney disease,and provide a certain theoretical basis for the pre...Objective:To review the possible mechanism and clinical application of the main components of Yuxingcao(Herba Houttuyniae)in the treatment of diabetic kidney disease,and provide a certain theoretical basis for the prevention and treatment of diabetic nephropathy with Yuxingcao(Herba Houttuyniae).Methods:This study viewed on etiology and pathogenesis,main components and pharmacology,and mechanismd of Yuxingcao(Herba Houttuyniae);Researched on clinical study on prevention and treatment of Diabetic kidney disease.Results:The mechanism of Yuxingcao(Herba Houttuyniae)was reducing urinary protein,regulating lipid metabolism,regulating the expression of fibrosis-related factors and improving expression of inflammatory chemokines.It had the effects of clearing away heat and detoxifying,eliminating carbuncle and discharging pus,diuretic and relieving stranguria.Conclusion:The literatures studied have proved that Yuxingcao(Herba Houttuyniae)has antibacterial,antiviral,antioxidant,anti-infammatory,diuretic,antitumor and other effects.It is one of the common used herbal medicines for diabetic kidney disease.展开更多
Objective： To research the protective effects of different extracts from Compound Houttuyniae Herba（CHH） and its mechanism through JAK/STAT-SOCS-1 signaling pathway.Methods： The normal group comprised db/m mice（n...Objective： To research the protective effects of different extracts from Compound Houttuyniae Herba（CHH） and its mechanism through JAK/STAT-SOCS-1 signaling pathway.Methods： The normal group comprised db/m mice（n = 8）. db/db mice were randomly divided into seven groups with eight mice in each group according to the applied treatment method： model group, metformin hydrochloride（MH） group, AG490 group, water extract（WE） group, ethanol extract（EE） group,volatile oil（VO） group, and mixture（MG） group（mixture of above three extract） of CHH. After 8 weeks,the general status, biochemical indicators, and renal histological changes in the mice were evaluated,the total RNA and protein were collected and RT-PCR method was used to examine the effect on mRNA expression of JAK2, STAT3, SOCS1, and Western blot method was used to detect the protein expression of JAK2, P-JAK2, STAT3, P-STAT3, SOCS1, c-fos, and c-jun. Immunofluorescence was used to observe the protein expression of c-fos and c-jun in kidney tissue.Results： Compared with normal group, the serum level of TGF-β1, FN of EE, VO, and MG groups were decreased（P 〈 0.05）. Renal function was also improved but not significantly. Also, the renal histology was improved especially in the mixture group. The protein expression of JAK2, STAT3, P-JAK2, P-STAT3, and the genetic expression of JAK2 and STAT3 in kidney tissue were significantly increased in the model group（P 〈 0.05）. Compared with the model group, the expression of P-JAK2 and P-STAT3 was down-regulated in treatment groups; The expression of SOCS-1 of VO and mixture groups were elevated（P 〈 0.05）.Conclusion： CHH has beneficial effects on diabetic renal injury, which may protect and improve the kidney function and reduce urinary protein, maintain the integrity of the kidney structure and function.展开更多
Objective Houttuynia cordata Thunb and Fructus Arctii are two traditional Chinese medicine（TCM） compounds widely used for reducing heat and toxins in the body. In addition, Fructus Arctii has been used in clinical t...Objective Houttuynia cordata Thunb and Fructus Arctii are two traditional Chinese medicine（TCM） compounds widely used for reducing heat and toxins in the body. In addition, Fructus Arctii has been used in clinical therapy for diabetic nephropathy. On the basis of the TCM theory of heat in diabetes mellitus and the clinical efficacy of Fructus Arctii, the two compounds were used to develop a formula named Compound Herba Houttuyniae. This study evaluated the renoprotective effects of different Compound Herba Houttuyniae extracts and assessed their mechanisms through the Janus kinase/signal transducer and activator of transcription（JAK/STAT）–suppressors of cytokine signaling（SOCS）-1 pathway. Methods The normal group comprised db/m mice（n = 8）; db/db mice were randomly divided into six groups according to the applied treatment method： model group（no treatment）, AG490 group, petroleum ether extract（PEE） group, ethyl acetate extract（EAE） group, N-butanol extract（NE） group, and water extract（WE） group（n = 6 in each group）. The general status, biochemical indicators, and renal histological changes in the mice were evaluated, and the JAK/STAT–SOCS-1 pathway was assessed. Results The NE and WE groups showed a decrease in 24-h urinary protein and albumin levels as well as serum uric acid and fibronectin levels but showed no changes in fasting plasma glucose and hemoglobin A1 c levels. The protein expression of JAK2, STAT3, P-JAK2, and P-STAT3 and the gene expression of JAK2 and STAT3 in kidney tissues were significantly increased（P 〈 0.05） in the model group. Compared with the model group, the expression of P-JAK2 and P-STAT3 was downregulated in the extract treatment groups. The expression of SOCS-1 was increased in the NE and WE groups（P 〈 0.05）. However, the expression of JAK2, STAT3, JAK2 m RNA, and STAT3 m RNA in the treatment groups did not show any significant differences. Conclusion Compound Herba Houttuyniae showed renoprotective effects in db/db mice, and the strongest effects were observed in the WE and NE groups. The underlying mechanism for these effects might involve the regulation of the JAK/STAT–SOCS-1 pathway.展开更多
基金Doctoral Startup Fund Project of Guizhou University of Traditional Chinese Medicine(3043-043190029)。
文摘Objective:To review the possible mechanism and clinical application of the main components of Yuxingcao(Herba Houttuyniae)in the treatment of diabetic kidney disease,and provide a certain theoretical basis for the prevention and treatment of diabetic nephropathy with Yuxingcao(Herba Houttuyniae).Methods:This study viewed on etiology and pathogenesis,main components and pharmacology,and mechanismd of Yuxingcao(Herba Houttuyniae);Researched on clinical study on prevention and treatment of Diabetic kidney disease.Results:The mechanism of Yuxingcao(Herba Houttuyniae)was reducing urinary protein,regulating lipid metabolism,regulating the expression of fibrosis-related factors and improving expression of inflammatory chemokines.It had the effects of clearing away heat and detoxifying,eliminating carbuncle and discharging pus,diuretic and relieving stranguria.Conclusion:The literatures studied have proved that Yuxingcao(Herba Houttuyniae)has antibacterial,antiviral,antioxidant,anti-infammatory,diuretic,antitumor and other effects.It is one of the common used herbal medicines for diabetic kidney disease.
基金supported by the National Natural Science Foundation of China(No.81403345)Program of Shanghai Municipal Education Commission(No.14YZ059)
文摘Objective： To research the protective effects of different extracts from Compound Houttuyniae Herba（CHH） and its mechanism through JAK/STAT-SOCS-1 signaling pathway.Methods： The normal group comprised db/m mice（n = 8）. db/db mice were randomly divided into seven groups with eight mice in each group according to the applied treatment method： model group, metformin hydrochloride（MH） group, AG490 group, water extract（WE） group, ethanol extract（EE） group,volatile oil（VO） group, and mixture（MG） group（mixture of above three extract） of CHH. After 8 weeks,the general status, biochemical indicators, and renal histological changes in the mice were evaluated,the total RNA and protein were collected and RT-PCR method was used to examine the effect on mRNA expression of JAK2, STAT3, SOCS1, and Western blot method was used to detect the protein expression of JAK2, P-JAK2, STAT3, P-STAT3, SOCS1, c-fos, and c-jun. Immunofluorescence was used to observe the protein expression of c-fos and c-jun in kidney tissue.Results： Compared with normal group, the serum level of TGF-β1, FN of EE, VO, and MG groups were decreased（P 〈 0.05）. Renal function was also improved but not significantly. Also, the renal histology was improved especially in the mixture group. The protein expression of JAK2, STAT3, P-JAK2, P-STAT3, and the genetic expression of JAK2 and STAT3 in kidney tissue were significantly increased in the model group（P 〈 0.05）. Compared with the model group, the expression of P-JAK2 and P-STAT3 was down-regulated in treatment groups; The expression of SOCS-1 of VO and mixture groups were elevated（P 〈 0.05）.Conclusion： CHH has beneficial effects on diabetic renal injury, which may protect and improve the kidney function and reduce urinary protein, maintain the integrity of the kidney structure and function.
基金National Natural Science Foundation of China(No.81403345)Innovation Program of Shanghai Municipal Education Commission(No.14YZ059)
文摘Objective Houttuynia cordata Thunb and Fructus Arctii are two traditional Chinese medicine（TCM） compounds widely used for reducing heat and toxins in the body. In addition, Fructus Arctii has been used in clinical therapy for diabetic nephropathy. On the basis of the TCM theory of heat in diabetes mellitus and the clinical efficacy of Fructus Arctii, the two compounds were used to develop a formula named Compound Herba Houttuyniae. This study evaluated the renoprotective effects of different Compound Herba Houttuyniae extracts and assessed their mechanisms through the Janus kinase/signal transducer and activator of transcription（JAK/STAT）–suppressors of cytokine signaling（SOCS）-1 pathway. Methods The normal group comprised db/m mice（n = 8）; db/db mice were randomly divided into six groups according to the applied treatment method： model group（no treatment）, AG490 group, petroleum ether extract（PEE） group, ethyl acetate extract（EAE） group, N-butanol extract（NE） group, and water extract（WE） group（n = 6 in each group）. The general status, biochemical indicators, and renal histological changes in the mice were evaluated, and the JAK/STAT–SOCS-1 pathway was assessed. Results The NE and WE groups showed a decrease in 24-h urinary protein and albumin levels as well as serum uric acid and fibronectin levels but showed no changes in fasting plasma glucose and hemoglobin A1 c levels. The protein expression of JAK2, STAT3, P-JAK2, and P-STAT3 and the gene expression of JAK2 and STAT3 in kidney tissues were significantly increased（P 〈 0.05） in the model group. Compared with the model group, the expression of P-JAK2 and P-STAT3 was downregulated in the extract treatment groups. The expression of SOCS-1 was increased in the NE and WE groups（P 〈 0.05）. However, the expression of JAK2, STAT3, JAK2 m RNA, and STAT3 m RNA in the treatment groups did not show any significant differences. Conclusion Compound Herba Houttuyniae showed renoprotective effects in db/db mice, and the strongest effects were observed in the WE and NE groups. The underlying mechanism for these effects might involve the regulation of the JAK/STAT–SOCS-1 pathway.
