BACKGROUND Ulcerative colitis(UC),also known as chronic nonspecific UC,is an inflammatory bowel disease characterized by diffuse colonic mucosal inflammation.The incidence and prevalence of UC have risen markedly,and ...BACKGROUND Ulcerative colitis(UC),also known as chronic nonspecific UC,is an inflammatory bowel disease characterized by diffuse colonic mucosal inflammation.The incidence and prevalence of UC have risen markedly,and the disease seriously affects the quality of life of patients,and poses a great burden on the world health care infrastructure and economy.CASE SUMMARY We present a 60-year-old man who had ulcerative colitis for more than 10 years,with recurrent abdominal pain,bloody diarrhea with mucopurulent stool.The treatments with sulfasalazine,mesalazine,and traditional Chinese medicine were not effective,and herbs-partitioned moxibustion(HPM)was then applied at“Zhongwan”(RN12),“Tianshu”(ST25),and“Qihai”(RN6)once a day for about 30 min,3 times per week,for 6 mo.His main clinical symptoms of abdominal pain,bloody diarrhea with mucopurulent stool gradually improved,and the mucosa had nearly healed,as observed under endoscopy by the 6th mo.The patient’s condition was alleviated without relapsing during the subsequent 3-mo follow-up period.HPM showed a significant effect in the treatment of ulcerative colitis in this case,and the effect would help the patient to maintain remission for at least 3 mo.CONCLUSION A series of symptoms of this UC patient significantly improved with the treatment of HPM.展开更多
BACKGROUND A20 inhibits intestinal epithelial cell apoptosis in Crohn's disease, and herbspartitioned moxibustion(HPM) has been demonstrated to be an effective treatment for Crohn's disease. However, the mecha...BACKGROUND A20 inhibits intestinal epithelial cell apoptosis in Crohn's disease, and herbspartitioned moxibustion(HPM) has been demonstrated to be an effective treatment for Crohn's disease. However, the mechanism by which HPM reduces intestinal epithelial cell apoptosis in Crohn's disease has not been thoroughly elucidated to date.AIM To elucidate whether HPM exerts its effects by upregulating A20 to affect intestinal epithelial cell apoptosis in a Crohn's disease mouse model.METHODS In this study, mice with A20 deletion in intestinal epithelial cells(A20 IEC-KO) were utilized to establish a Crohn's disease mouse model with 2,4,6-trinitrobenzenesulfonic acid(TNBS) administration, as well as wild-type mice. Mice were randomly divided into normal control(NC), model control(MC), mesalazine(MESA), and HPM groups. The morphology of the colonic mucosa was observed by hematoxylin-eosin staining, and serum endotoxin and apoptosis of epithelial cells were evaluated by enzyme-linked immunosorbent assay and terminal dUTP nick-end labeling assay accordingly. The protein expression levels of A20 and tumor necrosis factor receptor 1(TNFR1)-related signaling molecules were evaluated by Western blot, and co-expression of A20 and TNFR1-associated death domain(TRADD) and co-expression of A20 and receptor-interacting protein 1(RIP1) were observed by double immunofluorescence staining.RESULTS The intestinal epithelial barrier was noted to have an improvement in the HPM group of wild-type(WT) mice compared with that in A20 IEC-KO mice. Compared with A20 IEC-KO HPM mice, serum endotoxin levels and apoptosis percentages were decreased(P < 0.01), A20 expression levels were increased(P < 0.01), and expression of TNFR1, TRADDD, and RIP1 was decreased in the HPM group of WT mice(PTNFR1 < 0.05, PTRADD < 0.01, PRIP1 < 0.01). Both of the co-expression of A20/TRADD and A20/RIP1 showed a predominantly yellow fluorescence in the HPM group of WT mice, while a predominantly red fluorescence was noted in the HPM group of A20 IEC-KO mice.CONCLUSION Our findings suggest that HPM in treating Crohn's disease functions possibly via upregulation of the A20 expression level, resulting in downregulation of TNFR1,TRADD, and RIP1 to alleviate increased cell apoptosis in the intestinal epithelial barrier in Crohn's disease.展开更多
本文介绍了名为ACU&MOX-DATA(Acupuncture&moxibustion-deep analysis to treat accurately)的针灸多组学异源数据融合分析网络平台(http://cbcb.cdutcm.edu.cn/ACU&MOX-DATA/)。该平台旨在整合分析针灸相关疾病、临床和实...本文介绍了名为ACU&MOX-DATA(Acupuncture&moxibustion-deep analysis to treat accurately)的针灸多组学异源数据融合分析网络平台(http://cbcb.cdutcm.edu.cn/ACU&MOX-DATA/)。该平台旨在整合分析针灸相关疾病、临床和实验室获得的异源、多层次数据,其典型特点是在中医理论指导下,收集针灸研究所获得的各种观测指标,通过异源、多层次数据融合分析的方法,实现对针灸学领域关键科学问题的集成分析和可视化解读。本文介绍了该平台目前已有的功能及未来的研究计划。展开更多
基金Clinical Research Plan of Shanghai Hospital Development Center,No.16 CR4023ANational Natural Science Foundation of China,No.81574081 and No.81674074+2 种基金Shanghai Sailing Program,No.17YF1417600Chinese Medicine Inheritance and Innovation“100 Million”Talent Project(Qi Huang Scholar),No.2018284the Project of Shanghai Municipal Health Commission,No.201840096。
文摘BACKGROUND Ulcerative colitis(UC),also known as chronic nonspecific UC,is an inflammatory bowel disease characterized by diffuse colonic mucosal inflammation.The incidence and prevalence of UC have risen markedly,and the disease seriously affects the quality of life of patients,and poses a great burden on the world health care infrastructure and economy.CASE SUMMARY We present a 60-year-old man who had ulcerative colitis for more than 10 years,with recurrent abdominal pain,bloody diarrhea with mucopurulent stool.The treatments with sulfasalazine,mesalazine,and traditional Chinese medicine were not effective,and herbs-partitioned moxibustion(HPM)was then applied at“Zhongwan”(RN12),“Tianshu”(ST25),and“Qihai”(RN6)once a day for about 30 min,3 times per week,for 6 mo.His main clinical symptoms of abdominal pain,bloody diarrhea with mucopurulent stool gradually improved,and the mucosa had nearly healed,as observed under endoscopy by the 6th mo.The patient’s condition was alleviated without relapsing during the subsequent 3-mo follow-up period.HPM showed a significant effect in the treatment of ulcerative colitis in this case,and the effect would help the patient to maintain remission for at least 3 mo.CONCLUSION A series of symptoms of this UC patient significantly improved with the treatment of HPM.
基金Supported by National Natural Science Foundation of China,No.81273844 and No.81473757National Key Basic Research Program of China(973 Program),No.2015CB554500Shanghai Rising-Star Program,No.16QA1403400
文摘BACKGROUND A20 inhibits intestinal epithelial cell apoptosis in Crohn's disease, and herbspartitioned moxibustion(HPM) has been demonstrated to be an effective treatment for Crohn's disease. However, the mechanism by which HPM reduces intestinal epithelial cell apoptosis in Crohn's disease has not been thoroughly elucidated to date.AIM To elucidate whether HPM exerts its effects by upregulating A20 to affect intestinal epithelial cell apoptosis in a Crohn's disease mouse model.METHODS In this study, mice with A20 deletion in intestinal epithelial cells(A20 IEC-KO) were utilized to establish a Crohn's disease mouse model with 2,4,6-trinitrobenzenesulfonic acid(TNBS) administration, as well as wild-type mice. Mice were randomly divided into normal control(NC), model control(MC), mesalazine(MESA), and HPM groups. The morphology of the colonic mucosa was observed by hematoxylin-eosin staining, and serum endotoxin and apoptosis of epithelial cells were evaluated by enzyme-linked immunosorbent assay and terminal dUTP nick-end labeling assay accordingly. The protein expression levels of A20 and tumor necrosis factor receptor 1(TNFR1)-related signaling molecules were evaluated by Western blot, and co-expression of A20 and TNFR1-associated death domain(TRADD) and co-expression of A20 and receptor-interacting protein 1(RIP1) were observed by double immunofluorescence staining.RESULTS The intestinal epithelial barrier was noted to have an improvement in the HPM group of wild-type(WT) mice compared with that in A20 IEC-KO mice. Compared with A20 IEC-KO HPM mice, serum endotoxin levels and apoptosis percentages were decreased(P < 0.01), A20 expression levels were increased(P < 0.01), and expression of TNFR1, TRADDD, and RIP1 was decreased in the HPM group of WT mice(PTNFR1 < 0.05, PTRADD < 0.01, PRIP1 < 0.01). Both of the co-expression of A20/TRADD and A20/RIP1 showed a predominantly yellow fluorescence in the HPM group of WT mice, while a predominantly red fluorescence was noted in the HPM group of A20 IEC-KO mice.CONCLUSION Our findings suggest that HPM in treating Crohn's disease functions possibly via upregulation of the A20 expression level, resulting in downregulation of TNFR1,TRADD, and RIP1 to alleviate increased cell apoptosis in the intestinal epithelial barrier in Crohn's disease.
基金National Key R&D Program of China(No.2019YFC1709001,2022YFC3500703)Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine.(No.ZYYCXTD-D-202003)+1 种基金Fund of Science and Technology Department of Sichuan Province,China(No.2021ZYD0081,2022ZDZX0033)Xinglin Youth Innovation Team Project of Chengdu University of Traditional Chinese Medicine(QNTD2022003).
文摘本文介绍了名为ACU&MOX-DATA(Acupuncture&moxibustion-deep analysis to treat accurately)的针灸多组学异源数据融合分析网络平台(http://cbcb.cdutcm.edu.cn/ACU&MOX-DATA/)。该平台旨在整合分析针灸相关疾病、临床和实验室获得的异源、多层次数据,其典型特点是在中医理论指导下,收集针灸研究所获得的各种观测指标,通过异源、多层次数据融合分析的方法,实现对针灸学领域关键科学问题的集成分析和可视化解读。本文介绍了该平台目前已有的功能及未来的研究计划。
