[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the qu...[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the quercetin was quantitatively analyzed by HPLC.[Results]There was a good linear relationship between the peak area(A)and the content(M)of quercetin in the range of 0.048-0.168μg,A=2615.5M+1.5286,r=0.9999.The method was applied to the determination of ethanol extract of H.littoralis leaves,the average recovery rate was 98.77%and RSD was 1.57%.[Conclusions]The method is simple,convenient,reproducible and accurate,and provides an idea for the determination of quercetin in leaves of H.littoralis.展开更多
Inundation associated with flooding and sea level rise is predicted to have major impacts on survival, growth and productivity of mangroves. A manipulative mesocosm experiment was conducted to assess whether nutrient ...Inundation associated with flooding and sea level rise is predicted to have major impacts on survival, growth and productivity of mangroves. A manipulative mesocosm experiment was conducted to assess whether nutrient enrichment enhances growth and photosynthesis of waterlogged and salinity stressed seedlings of the mangrove Heritiera littoralis Dryand. Seedlings were fertilized with a three-component granular farm NPK (Nitrogen-Phosphorous-Potassium, 20:20:20) at varied loads under waterlogging with four levels of salinity. Growth was assessed through biomass accumulation, and photosynthesis through photosynthetic quantum yield and leaf Chl content. When seedlings were exposed to both nutrient enrichment and increased salinity, a significant shift of biomass to roots was observed. Seedlings had low value of Chl a/b ratio and photosynthetic distraction resulting in mortality was high in the highest salinity and nutrient levels. These results are useful in explaining failure of regeneration in natural stands of riverine and freshwater bound mangroves following unprecedented flooding events that accrue land based nutrient loads into estuaries and deltas.展开更多
基金Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GXZYKF2019-7).
文摘[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the quercetin was quantitatively analyzed by HPLC.[Results]There was a good linear relationship between the peak area(A)and the content(M)of quercetin in the range of 0.048-0.168μg,A=2615.5M+1.5286,r=0.9999.The method was applied to the determination of ethanol extract of H.littoralis leaves,the average recovery rate was 98.77%and RSD was 1.57%.[Conclusions]The method is simple,convenient,reproducible and accurate,and provides an idea for the determination of quercetin in leaves of H.littoralis.
文摘Inundation associated with flooding and sea level rise is predicted to have major impacts on survival, growth and productivity of mangroves. A manipulative mesocosm experiment was conducted to assess whether nutrient enrichment enhances growth and photosynthesis of waterlogged and salinity stressed seedlings of the mangrove Heritiera littoralis Dryand. Seedlings were fertilized with a three-component granular farm NPK (Nitrogen-Phosphorous-Potassium, 20:20:20) at varied loads under waterlogging with four levels of salinity. Growth was assessed through biomass accumulation, and photosynthesis through photosynthetic quantum yield and leaf Chl content. When seedlings were exposed to both nutrient enrichment and increased salinity, a significant shift of biomass to roots was observed. Seedlings had low value of Chl a/b ratio and photosynthetic distraction resulting in mortality was high in the highest salinity and nutrient levels. These results are useful in explaining failure of regeneration in natural stands of riverine and freshwater bound mangroves following unprecedented flooding events that accrue land based nutrient loads into estuaries and deltas.
