Differences in chlorophyll fluorescence parameters and water content in heteromorphic leaves of Populus euphratica from Inner Mongolia, China

We studied three typical heteromorphic leaves of Populus euphratica trees growing in the Wuhai region of Inner Mongolia,China,i.e.,lanceolate,broad-ovate and dentate broad-ovate leaves and mainly focused on the changes in chlorophyll fluorescence parameters and free water and bound water content.The results show that the values of Fm（maximal fluorescence yield）,Fv/Fm（maximum photochemical quantum yield of PSII） and Fv/F0（potential quantum efficiency of PSII） of lanceolate leaves were the least on young trees,while these parameters were the least on the ovate leaves of old trees.Compared with young trees,the free water content of heteromorphic leaves of old trees increased significantly,i.e.,by 78.94% in lanceolate leaves and in the leaves of broad-ovate and dentate broad-ovate by 10.99% and 10.60%,respectively.Correlation analysis showed that free water content is significantly related to Fv/Fm and Fv/F0 in young trees,while the relationship of total water content with Fv/Fm and Fv/F0 is positive in old trees.

A New Two-Parameter Heteromorphic Elliptic Equation: Properties and Applications

The ellipse and the superellipse are both planar closed curves with a double axis of symmetry. Here we show the isoconcentration contour of the simplified two-dimensional advection-diffusion equation from a stable line source in the center of a wide river. A new two-parameter heteromorphic elliptic equation with a single axis of symmetry is defined. The values of heights, at the point of the maximum width and that of the centroid of the heteromorphic ellipse, are derived through mathematical analysis. Taking the compression coefficient θ = b/a = 1 as the criterion, the shape classification of H-type, Standard-type and W-type for heteromorphic ellipse have been given. The area formula, the perimeter theorem, and the radius of curvature of heteromorphic ellipses, and the geometric properties of the rotating body are subsequently proposed. An illustrative analysis shows that the inner contour curve of a heteromorphic elliptic tunnel has obvious advantages over the multiple- arc splicing cross section. This work demonstrates that the heteromorphic ellipses have extensive prospects of application in all categories of tunnels, liquid transport tanks, aircraft and submarines, bridges, buildings, furniture, and crafts.

Differences Between Solitary Cells and Colonial Cells in the Heteromorphic Life Cycle of Phaeocystis globosa: Morphology, Physiology, and Transcriptome

Large-scale blooms of Phaeocystis globosa have caused serious damage to marine ecosystems in coastal waters of China.Phaeocystis blooms depend on the competitive advantage of their heteromorphic life history:colony formation has the benefit of resisting herbivory by zooplankton,and solitary cells can absorb nutrients rapidly.In order to better understand the mechanisms underlying the metabolic differences between the two types of cells,morphological observations,rapid light curve analysis,fatty acid profiling,and transcriptome assessment were conducted in the laboratory.The rapid light curve of colonial cells was higher than that of solitary cells,which indicated that colonial cells had higher CO2 fixation capacity.The fatty acid level of colonial cells was evidently lower than that of solitary cells,which is consistent with down-regulated synthesis of fatty acids and up-regulated degradation of fatty acids in the transcriptome.ATP-binding cassette transporters,the TCA cycle,and biosynthesis of exopolysaccharides(EPS)also displayed obvious differences.In summary,colonial cells have stronger carbon fixation capacity.They do not synthesize fatty acids as energy storage materials but secrete EPS,which might be one of the mechanisms of colony formation.Here we present a physiological and molecular overview of the differences between solitary cells and colonial cells and thereby provide further insight to help unravel the mechanisms that help Phaeocystis globosa adapt to different environments.

Elevated CO_(2) increases shoot growth but not root growth and C:N:P stoichiometry of Suaeda aralocaspica plants

The purpose of the current study was to investigate the eco-physiological responses,in terms of growth and C:N:P stoichiometry of plants cultured from dimorphic seeds of a single-cell C4 annual Suaeda aralocaspica(Bunge)Freitag and Schütze under elevated CO_(2).A climatic chamber experiment was conducted to examine the effects of ambient(720μg/L)and CO_(2)-enriched(1440μg/L)treatments on these responses in S.aralocaspica at vegetative and reproductive stages in 2012.Result showed that elevated CO_(2) significantly increased shoot dry weight,but decreased N:P ratio at both growth stages.Plants grown from dimorphic seeds did not exhibit significant differences in growth and C:N:P stoichiometric characteristics.The transition from vegetation to reproductive stage significantly increased shoot:root ratio,N and P contents,but decreased C:N,C:P and N:P ratios,and did not affect shoot dry weight.Moreover,our results indicate that the changes in N:P and C:N ratios between ambient and elevated CO_(2) are mainly caused by the decrease of N content under elevated CO_(2).These results provide an insight into nutritional metabolism of single-cell C4 plants under climate change.

Stigma-Specific Comparative Proteomic Analysis Reveals the Distyly Response to Self-Incompatibility in Plumbago auriculata Lam

In plants,heteromorphic self-incompatibility(HetSI)is a strategy for avoiding self-pollination and promoting outcrossing,and during this process,numerous protein-protein interaction events occur between the pistil and pollen.Previous studies in Primula and Fagopyrum that focused on HetSI systems have provided interesting insights;however,the molecular mechanism underlying HetSI remains largely unknown.In this study,we profiled the proteome of Plumbago auriculata stigmas before and after self-incompatible(SI)and self-compatible(SC)pollination.Comparative analyses were conducted by 4D-DIA(Four-dimensional data independent acquisition),a promising technology that increases the sensitivity and reduces the spectral complexity of proteomic analysis by adding a fourth dimension,ion mobility.The results revealed 33387 peptides and 5311 proteins in all samples.The pathways in which the differentially expressed proteins(DEPs)identified in the P×P(Pin style self-pollinated with pin pollen)vs.PS(Pin style)and T×T(Thrum style self-pollinated with thrum pollen)vs.TS(Thrum style)comparisons were significantly enriched were biosynthesis of secondary metabolites and pentose and glucuronate interconversions.In the P×T(Pin style cross-pollinated with thrum pollen)vs.PS and T×P(Thrum style cross-pollinated with pin pollen)vs.TS comparison,the top three pathways were biosynthesis of secondary metabolites,pentose and glucuronate interconversions,and phenylpropanoid biosynthesis.The phenylpropanoid biosynthesis,cutin,suberine and wax biosynthesis,and flavonoid biosynthesis pathways were enriched in the P×T vs.P×P comparison,and starch and sucrose metabolism,glycerophospholipid metabolism,and alpha-linolenic acid metabolism were abundant in the T×T vs.T×P comparison.The enriched pathways between PS and TS were the biosynthesis of secondary metabolites,phenylpropanoid biosynthesis,and pentose and glucuronate interconversion.Self-incompatibility protein S1(SI S1),Mitogen-activated protein kinase 3/4(MPK3/4),Mitogen-activated protein kinase kinase 2/3(M2K2/3),Exocyst complex component EXO70A1(E70A1)and Thioredoxin H1/2(TRXH1/2)were found to be HetSI-related candidates,and O-fucosyltransferase 23(OFT23),3-ketoacyl-CoA synthase 6(KCS6),Receptor-like protein kinase FERONIA(FERON),Fimbrin-5(FIMB5),Pollen-specific leucine-rich repeat extensin-like protein 4(PLRX4),Transcription initiation factor IIB-2(TF2B2)and Pectinesterase 1(AL11A),etc.,were identified as other regulatory transducers.These findings combined with our morphological and reactive oxygen species(ROS)intensity analyses indicate that P.auriculata has typical dry-stigmas and that the HetSI mechanism might differ between the pin and thrum.SI S1 might be the key factor in HetSI,and ROS are overexpressed during SC pollination to rapidly activate the mitogen-activated protein kinase(MAPK)-mediated phosphorylation of E70A1 to maintain stigma receptivity in plants with HetSI.

Expanding and improving the LEADS code for dynamics design and multiparticle simulation

In order to calculate the effect on the beam caused by an irregular accelerator element, we have expanded and improved the Linear and Electrostatic Accelerator Dynamics Simulation （LEADS） code. To achieve better calculation precision, the element was divided into lots of equal intervals. In order to simplify the calculation process, a one-dimensional field is simulated and the Lorenz equation is used directly. A one-dimensional field can be imported into the LEADS code. The heteromorphic quadrupole is invented and its field is simulated and optimized using the POISSON code. As examples, the effect on the beam caused by the heteromorphic quadrupole and octupole is simulated.

Nucleolar Organizer Regions in Oreochromis nilotica Chromosomes

The nucleolar organizer regions (NORs) in the mitotic chromosomes of tilapia Oreochromis niloticus were analyzed using silver nitrate staining Ag detected NORs(Ag NORs) in the chromosomes of the tilapia exhibited heteromorphism. Individual Ag NORs sizes varied on different chromosomes, and the number of Ag NOR per cell varied from three to six. Among the examined 100 cells, 71% of the gnomes carried four Ag NORs, 12% carried 3,11% carried 5, and 6% carried 6. The variance occurred not only among different cells but also among different cells from the same source. The satellite association between homologous chromosomes in No 7 was also observed in 27% of the metaphase chromosome preparations. The heteromorphic properties of Ag NORs were discussed.